2. Metabolic Enzyme/Protease
  3. Proteasome
  4. Ac-ANW-AMC

Ac-ANW-AMC is a fluorogenic substrate for immunoproteasome. Ac-ANW-AMC can be used to measure β5i activity (Ex=345 nm, Em=445 nm).

For research use only. We do not sell to patients.

Ac-ANW-AMC Chemical Structure

Ac-ANW-AMC Chemical Structure

CAS No. : 2357123-49-8

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1 mg USD 256 In-stock
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Based on 1 publication(s) in Google Scholar

Ac-ANW-AMC Related Antibodies

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Description

Ac-ANW-AMC is a fluorogenic substrate for immunoproteasome. Ac-ANW-AMC can be used to measure β5i activity (Ex=345 nm, Em=445 nm)[1][2].

IC50 & Target

β5i[1]
In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. For the proteasome activity assay, cells are lysed in the proteasome activity lysis buffer.
2. Cells are homogenized by passing the lysates 15 times through a 26G×1/2″ needle attached to a 1-mL syringe.
3. The lysates are then centrifuged at 12,000 rpm for 15 min. Protein concentrations of the samples are determined using the Bradford assay.
4. Proteasome activity is determined using model peptide substrates by measuring free Ac-ANW-AMC fluorescence on a TECAN infinite m200 fluorometer.
5. The fluorescence unquenched after hydrolysis by proteasomes is monitored every three minutes at 345 nm excitation and 445nm emission wavelengths at 30°C.
Note: Each sample is assayed in triplicate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac-ANW-AMC Related Antibodies
Molecular Weight

588.61

Formula

C30H32N6O7
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

460
Excitation (Ex)

380
SMILES

O=C1OC2=CC=C(C=C2C(C)=C1)NC([C@@H](NC([C@H](CC(N)=O)NC([C@H](C)NC(C)=O)=O)=O)CC3=CNC4=CC=CC=C34)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (169.89 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6989 mL 8.4946 mL 16.9892 mL
5 mM 0.3398 mL 1.6989 mL 3.3978 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6989 mL 8.4946 mL 16.9892 mL 42.4729 mL
5 mM 0.3398 mL 1.6989 mL 3.3978 mL 8.4946 mL
10 mM 0.1699 mL 0.8495 mL 1.6989 mL 4.2473 mL
15 mM 0.1133 mL 0.5663 mL 1.1326 mL 2.8315 mL
20 mM 0.0849 mL 0.4247 mL 0.8495 mL 2.1236 mL
25 mM 0.0680 mL 0.3398 mL 0.6796 mL 1.6989 mL
30 mM 0.0566 mL 0.2832 mL 0.5663 mL 1.4158 mL
40 mM 0.0425 mL 0.2124 mL 0.4247 mL 1.0618 mL
50 mM 0.0340 mL 0.1699 mL 0.3398 mL 0.8495 mL
60 mM 0.0283 mL 0.1416 mL 0.2832 mL 0.7079 mL
80 mM 0.0212 mL 0.1062 mL 0.2124 mL 0.5309 mL
100 mM 0.0170 mL 0.0849 mL 0.1699 mL 0.4247 mL
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Ac-ANW-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-ANW-AMC2357123-49-8ProteasomeFluorogenicpeptidesubstratesimmunoproteasomeInhibitorinhibitorinhibit

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