1. Anti-infection
  2. HBV Reverse Transcriptase
  3. Adefovir

Adefovir  (Synonyms: GS-0393; PMEA)

Cat. No.: HY-B1826 Purity: 99.55%
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Adefovir (GS-0393) is an adenosine monophosphate analog antiviral agent that after intracellular conversion to Adefovir diphosphate inhibits HBV DNA polymerase. Adefovir has an IC50 of 0.7 μM against HBV in the HepG2.2.15 cell line. Adefovir has good antiviral activity against several viruses, including HBV and herpesviruses.

For research use only. We do not sell to patients.

Adefovir Chemical Structure

Adefovir Chemical Structure

CAS No. : 106941-25-7

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Based on 1 publication(s) in Google Scholar

Other Forms of Adefovir:

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Description

Adefovir (GS-0393) is an adenosine monophosphate analog antiviral agent that after intracellular conversion to Adefovir diphosphate inhibits HBV DNA polymerase. Adefovir has an IC50 of 0.7 μM against HBV in the HepG2.2.15 cell line. Adefovir has good antiviral activity against several viruses, including HBV and herpesviruses[1][2][3].

IC50 & Target

HBV[1][2][3];
DNA polymerase[1][2]

Cellular Effect
Cell Line Type Value Description References
C-33-A IC50
7.8 μM
Compound: 1, PMEA
Antiproliferative activity against human C33A cells after 72 hrs by flow cytometric analysis
Antiproliferative activity against human C33A cells after 72 hrs by flow cytometric analysis
[PMID: 24686012]
C3H/3T3 EC50
0.53 μM
Compound: PMEA, Adefovir
Inhibition of murine sarcoma virus-induced transformation of mouse embryo fibroblast C3H/3T3 cells after 6 days
Inhibition of murine sarcoma virus-induced transformation of mouse embryo fibroblast C3H/3T3 cells after 6 days
[PMID: 18556209]
C3H/3T3 EC50
2.1 μM
Compound: PMEA
Antiviral against Moloney murine sarcoma virus infected in mouse C3H/3T3 cells assessed as inhibition of virus-induced cell transformation after 6 days post infection by microscopic analysis
Antiviral against Moloney murine sarcoma virus infected in mouse C3H/3T3 cells assessed as inhibition of virus-induced cell transformation after 6 days post infection by microscopic analysis
[PMID: 21429755]
C8166 EC50
1.7 μM
Compound: PMEA
Concentration required to inhibit p24 viral antigen production by 50% in C-8166 cells
Concentration required to inhibit p24 viral antigen production by 50% in C-8166 cells
10.1016/0960-894X(95)00208-B
C8166 EC50
7 μM
Compound: PMEA, Adefovir
Antiviral activity against 100 TCID50 wild-type Human immunodeficiency virus type 2 ROD infected in C8166 cells assessed as inhibition of syncytia formation after 4 days by microscopic analysis
Antiviral activity against 100 TCID50 wild-type Human immunodeficiency virus type 2 ROD infected in C8166 cells assessed as inhibition of syncytia formation after 4 days by microscopic analysis
[PMID: 21803462]
CCRF-CEM CC50
≥ 250 μM
Compound: PMEA
Cytotoxicity against human CEM cells assessed as inhibition of cell proliferation after 72 hrs by coulter counter analysis
Cytotoxicity against human CEM cells assessed as inhibition of cell proliferation after 72 hrs by coulter counter analysis
[PMID: 21429755]
CCRF-CEM EC50
10.4 μM
Compound: 1, PMEA, Adefovir
Antiviral activity against HIV 1 3B infected in CEM cells assessed as inhibition of virus-induced giant cell formation after 4 days by microscopy
Antiviral activity against HIV 1 3B infected in CEM cells assessed as inhibition of virus-induced giant cell formation after 4 days by microscopy
[PMID: 21565516]
CCRF-CEM CC50
164 μM
Compound: 1, PMEA, Adefovir
Cytotoxicity against human CEM cells assessed as growth inhibition after 3 days by coulter counting analysis
Cytotoxicity against human CEM cells assessed as growth inhibition after 3 days by coulter counting analysis
[PMID: 21565516]
CCRF-CEM EC50
18 μM
Compound: PMEA
In vitro anti -HIV activity tested against CEM cells infected with HIV by XTT assay
In vitro anti -HIV activity tested against CEM cells infected with HIV by XTT assay
[PMID: 1323678]
CCRF-CEM IC50
24 μM
Compound: 1, PMEA
Cytostatic activity against human CEM cells after 72 hrs by coulter counting analysis
Cytostatic activity against human CEM cells after 72 hrs by coulter counting analysis
[PMID: 24686012]
CCRF-CEM EC50
5.5 μM
Compound: PMEA, Adefovir
Antiviral activity against 100 TCID50 wild-type Human immunodeficiency virus 1 3B infected in CEM cells assessed as inhibition of syncytia formation after 4 days by microscopic analysis
Antiviral activity against 100 TCID50 wild-type Human immunodeficiency virus 1 3B infected in CEM cells assessed as inhibition of syncytia formation after 4 days by microscopic analysis
[PMID: 21803462]
CCRF-CEM EC50
7 μM
Compound: PMEA, adefovir
Antiviral activity against Human immunodeficiency virus type 2 ROD infected in human CEM cells assessed as HIV-induced CEM giant cell formation after 4 days by microscopy
Antiviral activity against Human immunodeficiency virus type 2 ROD infected in human CEM cells assessed as HIV-induced CEM giant cell formation after 4 days by microscopy
[PMID: 21745746]
CCRF-CEM EC50
7 μM
Compound: PMEA
Antiviral activity against HIV2 ROD infected in human CEM cells assessed as inhibition of virus-induced syncytium formation after 4 to 5 days by microscopic analysis
Antiviral activity against HIV2 ROD infected in human CEM cells assessed as inhibition of virus-induced syncytium formation after 4 to 5 days by microscopic analysis
[PMID: 21429755]
CCRF-CEM EC50
7.4 μM
Compound: PMEA, adefovir
Antiviral activity against Human immunodeficiency virus type1 3B infected in human CEM cells assessed as HIV-induced CEM giant cell formation after 4 days by microscopic analysis
Antiviral activity against Human immunodeficiency virus type1 3B infected in human CEM cells assessed as HIV-induced CEM giant cell formation after 4 days by microscopic analysis
[PMID: 21745746]
CCRF-CEM EC50
7.4 μM
Compound: PMEA
Antiviral activity against HIV1 3B infected in human CEM cells assessed as inhibition of virus-induced syncytium formation after 4 to 5 days by microscopic analysis
Antiviral activity against HIV1 3B infected in human CEM cells assessed as inhibition of virus-induced syncytium formation after 4 to 5 days by microscopic analysis
[PMID: 21429755]
CCRF-CEM EC50
8.2 μM
Compound: 1, PMEA
Antiviral activity against Human immunodeficiency virus type 2 ROD infected in human CEM cells assessed as inhibition of virus-induced giant cell formation after 4 to 5 days by microscopic analysis
Antiviral activity against Human immunodeficiency virus type 2 ROD infected in human CEM cells assessed as inhibition of virus-induced giant cell formation after 4 to 5 days by microscopic analysis
[PMID: 24686012]
CCRF-CEM EC50
8.4 μM
Compound: 1, PMEA
Antiviral activity against Human immunodeficiency virus 1 3B infected in human CEM cells assessed as inhibition of virus-induced giant cell formation after 4 to 5 days by microscopic analysis
Antiviral activity against Human immunodeficiency virus 1 3B infected in human CEM cells assessed as inhibition of virus-induced giant cell formation after 4 to 5 days by microscopic analysis
[PMID: 24686012]
CCRF-CEM EC50
8.6 μM
Compound: 1, PMEA, Adefovir
Antiviral activity against HIV 2 ROD infected in CEM cells assessed as inhibition of virus-induced giant cell formation after 4 days by microscopy
Antiviral activity against HIV 2 ROD infected in CEM cells assessed as inhibition of virus-induced giant cell formation after 4 days by microscopy
[PMID: 21565516]
CHO CC50
≥ 2000 μM
Compound: Adefovir
Cytotoxicity against CHO cells after 120 hrs by Cell-Titer Glo assay
Cytotoxicity against CHO cells after 120 hrs by Cell-Titer Glo assay
[PMID: 19001108]
CHO CC50
1.4 μM
Compound: Adefovir
Ratio of CC50 for CHO cells to CC50 for CHO cells expressing hOAT1 after 120 hrs by Cell-Titer Glo assay
Ratio of CC50 for CHO cells to CC50 for CHO cells expressing hOAT1 after 120 hrs by Cell-Titer Glo assay
[PMID: 19001108]
CHO CC50
3 μM
Compound: Adefovir
Cytotoxicity against CHO cells expressing hOAT1 after 120 hrs by Cell-Titer Glo assay
Cytotoxicity against CHO cells expressing hOAT1 after 120 hrs by Cell-Titer Glo assay
[PMID: 19001108]
H9 CC50
> 30 μM
Compound: PMEA, Adefovir
Cytotoxicity against PAP-activated human H9 cells on day 7 by MTT assay
Cytotoxicity against PAP-activated human H9 cells on day 7 by MTT assay
[PMID: 21803462]
H9 EC50
1.1 μM
Compound: PMEA, Adefovir
Antiviral activity against 125 TCID50 wild type HIV1 LAI infected in human H9 cells assessed as reduction in viral replication measured on day 7 post infection by reverse transcriptase activity
Antiviral activity against 125 TCID50 wild type HIV1 LAI infected in human H9 cells assessed as reduction in viral replication measured on day 7 post infection by reverse transcriptase activity
[PMID: 21803462]
H9 EC50
7.9 μM
Compound: PMEA, Adefovir
Antiviral activity against 6250 TCID50 wild type HIV1 LAI infected in human H9 cells assessed as reduction in viral replication measured on day 7 post infection by reverse transcriptase activity
Antiviral activity against 6250 TCID50 wild type HIV1 LAI infected in human H9 cells assessed as reduction in viral replication measured on day 7 post infection by reverse transcriptase activity
[PMID: 21803462]
HEK-293T CC50
> 100 μM
Compound: PMEA
Cytotoxicity against human 293T cells assessed as inhibition of cell proliferation by tetrazolium dye method
Cytotoxicity against human 293T cells assessed as inhibition of cell proliferation by tetrazolium dye method
[PMID: 17470654]
HEL 299 EC50
> 183 μM
Compound: 3a, PMEA
Antiviral activity against Camelpox virus CML1 infected in HEL299 cells assessed as inhibition of virus-induced cytopathic effect after 6 days by Giemsa staining
Antiviral activity against Camelpox virus CML1 infected in HEL299 cells assessed as inhibition of virus-induced cytopathic effect after 6 days by Giemsa staining
[PMID: 17893157]
HEL 299 EC50
> 183 μM
Compound: 3a, PMEA
Antiviral activity against Camelpox virus CML14 infected in HEL299 cells assessed as inhibition of virus-induced cytopathic effect after 6 days by Giemsa staining
Antiviral activity against Camelpox virus CML14 infected in HEL299 cells assessed as inhibition of virus-induced cytopathic effect after 6 days by Giemsa staining
[PMID: 17893157]
HEL 299 CC50
211 μM
Compound: 3a, PMEA
Cytotoxicity against HEL299 cells after 4 days by Z1 Coulter counting analysis
Cytotoxicity against HEL299 cells after 4 days by Z1 Coulter counting analysis
[PMID: 17893157]
HeLa CC50
> 250 μM
Compound: 1, PMEA, Adefovir
Cytotoxicity against human HeLa cells assessed as growth inhibition after 3 days by coulter counting analysis
Cytotoxicity against human HeLa cells assessed as growth inhibition after 3 days by coulter counting analysis
[PMID: 21565516]
HeLa IC50
181 μM
Compound: 1, PMEA
Cytostatic activity against human HeLa cells after 72 hrs by coulter counting analysis
Cytostatic activity against human HeLa cells after 72 hrs by coulter counting analysis
[PMID: 24686012]
HepG2 IC50
42.1 μM
Compound: PMEA, 1
Antiviral activity against Hepatitis B virus infected human HepG2 cells after 9 days by MTT assay
Antiviral activity against Hepatitis B virus infected human HepG2 cells after 9 days by MTT assay
[PMID: 17888662]
HepG2 2.2.15 IC50
0.517 μM
Compound: adefovir
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBV DNA replication after 9 days by PCR
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBV DNA replication after 9 days by PCR
[PMID: 20000776]
HepG2 2.2.15 IC50
0.517 μM
Compound: Adefovir
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of viral replication after 9 days by fluorescence PCR method
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of viral replication after 9 days by fluorescence PCR method
[PMID: 23353737]
HepG2 2.2.15 EC50
1.3 μM
Compound: Adefovir
Antiviral activity against wild type Hepatitis B virus infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization method
Antiviral activity against wild type Hepatitis B virus infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization method
[PMID: 21930377]
HepG2 2.2.15 EC50
1.6 μM
Compound: Adefovir
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase M204V mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization me
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase M204V mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization me
[PMID: 21930377]
HepG2 2.2.15 EC50
1.9 μM
Compound: Adefovir
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase M204I mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization me
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase M204I mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization me
[PMID: 21930377]
HepG2 2.2.15 EC50
2.1 μM
Compound: Adefovir
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase L180M/M204V double mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hyb
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase L180M/M204V double mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hyb
[PMID: 21930377]
HepG2 2.2.15 CC50
200 μM
Compound: Adefovir
Cytotoxicity in human HepG2.215 cells assessed as reduction in cell viability after 5 days by CCK8 assay
Cytotoxicity in human HepG2.215 cells assessed as reduction in cell viability after 5 days by CCK8 assay
[PMID: 28082068]
HepG2 2.2.15 IC50
215 μM
Compound: Adefovir
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as HBeAg secretion after 9 days by ELISA
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as HBeAg secretion after 9 days by ELISA
[PMID: 25650312]
HepG2 2.2.15 IC50
257 μM
Compound: Adefovir
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBsAg secretion after 9 days by ELISA
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBsAg secretion after 9 days by ELISA
[PMID: 25650312]
HepG2 2.2.15 IC50
286 μM
Compound: Adefovir
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBeAg production after 9 days by ELISA
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBeAg production after 9 days by ELISA
[PMID: 23353737]
HepG2 2.2.15 IC50
286 μM
Compound: adefovir
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBeAg level after 9 days by ELISA
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBeAg level after 9 days by ELISA
[PMID: 20000776]
HepG2 2.2.15 IC50
305 μM
Compound: Adefovir
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBsAg production after 9 days by ELISA
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBsAg production after 9 days by ELISA
[PMID: 23353737]
HepG2 2.2.15 IC50
305 μM
Compound: adefovir
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBsAg level after 9 days by ELISA
Antiviral activity against HBV infected in human HepG2(2.2.15) cells assessed as inhibition of HBsAg level after 9 days by ELISA
[PMID: 20000776]
HepG2 2.2.15 CC50
490 μM
Compound: Adefovir
Cytotoxicity against human HepG2.2.15 cells assessed as cell death after 9 days by MTS assay
Cytotoxicity against human HepG2.2.15 cells assessed as cell death after 9 days by MTS assay
[PMID: 25650312]
HepG2 2.2.15 EC50
5.5 μM
Compound: Adefovir
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase L180M mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization me
Antiviral activity against lamivudine-resistant Hepatitis B virus harboring reverse transcriptase L180M mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization me
[PMID: 21930377]
HepG2 2.2.15 CC50
540 μM
Compound: Adefovir
Cytotoxicity against human HepG2(2.2.15) cells compound treated for 48 hrs followed by incubation for 9 days by MTT assay
Cytotoxicity against human HepG2(2.2.15) cells compound treated for 48 hrs followed by incubation for 9 days by MTT assay
[PMID: 23353737]
HepG2 2.2.15 CC50
57 μM
Compound: adefovir
Cytotoxicity against HepG2.2.15 cells after 6 hrs by MTT assay
Cytotoxicity against HepG2.2.15 cells after 6 hrs by MTT assay
[PMID: 17499889]
HepG2 2.2.15 EC50
7.8 μM
Compound: Adefovir
Antiviral activity against adefovir-resistant Hepatitis B virus harboring reverse transcriptase N236T mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization meth
Antiviral activity against adefovir-resistant Hepatitis B virus harboring reverse transcriptase N236T mutant infected in human HepG2(2.2.15) cells assessed as inhibition of virus replication treated daily for 9 days by quantitative blot hybridization meth
[PMID: 21930377]
Huh-7 EC50
1.3 μM
Compound: adefovir
Antiviral activity against adefovir-resistant wild type HBV in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
Antiviral activity against adefovir-resistant wild type HBV in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
[PMID: 19072694]
Huh-7 EC50
1.5 μM
Compound: adefovir
Antiviral activity against adefovir-resistant HBV reverse transcriptase M204V mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
Antiviral activity against adefovir-resistant HBV reverse transcriptase M204V mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
[PMID: 19072694]
Huh-7 EC50
1.6 μM
Compound: adefovir
Antiviral activity against adefovir-resistant HBV reverse transcriptase L180M mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
Antiviral activity against adefovir-resistant HBV reverse transcriptase L180M mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
[PMID: 19072694]
Huh-7 EC50
1.6 μM
Compound: adefovir
Antiviral activity against adefovir-resistant HBV reverse transcriptase LM/reverse transcriptase MV double mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
Antiviral activity against adefovir-resistant HBV reverse transcriptase LM/reverse transcriptase MV double mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
[PMID: 19072694]
Huh-7 EC50
1.8 μM
Compound: adefovir
Antiviral activity against adefovir-resistant HBV reverse transcriptase M204I mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
Antiviral activity against adefovir-resistant HBV reverse transcriptase M204I mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
[PMID: 19072694]
Huh-7 EC50
6 μM
Compound: PMEA, Adefovir
Antiviral activity against Hepatitis B virus infected in human HuH7 cells assessed as reduction in viral DNA level treated day 3 to day 8 post transfection by real time quantitative PCR analysis
Antiviral activity against Hepatitis B virus infected in human HuH7 cells assessed as reduction in viral DNA level treated day 3 to day 8 post transfection by real time quantitative PCR analysis
[PMID: 21803462]
Huh-7 EC50
7.7 μM
Compound: adefovir
Antiviral activity against adefovir-resistant HBV reverse transcriptase 236T mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
Antiviral activity against adefovir-resistant HBV reverse transcriptase 236T mutant in human Huh7 cells after 4 days by intracellular HBV DNA replication assay
[PMID: 19072694]
L1210 IC50
3 μM
Compound: 1, PMEA
Cytostatic activity against mouse L1210 cells after 48 hrs by coulter counting analysis
Cytostatic activity against mouse L1210 cells after 48 hrs by coulter counting analysis
[PMID: 24686012]
MT4 EC50
22 μM
Compound: PMEA
Concentration required to inhibit HIV-1-induced cytopathic effect by 50% in MT-4 cells
Concentration required to inhibit HIV-1-induced cytopathic effect by 50% in MT-4 cells
10.1016/0960-894X(95)00208-B
PBMC CC50
> 30 μM
Compound: PMEA, Adefovir
Cytotoxicity against PAP-activated human PBMC on day 7 by MTT assay
Cytotoxicity against PAP-activated human PBMC on day 7 by MTT assay
[PMID: 21803462]
Vero IC50
119 μM
Compound: 1
Concentration that reduced plaque formation by 50% was measured in HSV-2 infected vero cells in vitro
Concentration that reduced plaque formation by 50% was measured in HSV-2 infected vero cells in vitro
[PMID: 8021925]
WI-38 CC50
124.7 μM
Compound: PMEA
Cytotoxicity against human WI38 cells by neutral red assay
Cytotoxicity against human WI38 cells by neutral red assay
[PMID: 18285481]
WI-38 EC50
95.8 μM
Compound: PMEA
Antimicrobial activity against BK polyomavirus ATCC VR837 infected in human WI38 cells assessed as reduction in viral titer after 7 days by PCR analysis
Antimicrobial activity against BK polyomavirus ATCC VR837 infected in human WI38 cells assessed as reduction in viral titer after 7 days by PCR analysis
[PMID: 18285481]
In Vitro

Studies to elucidate the mechanism of action of Adefovir against herpesvirus replication reveals that the phosphorylation of Adefovir occurred intracellularly and is carried out by host cellular enzymes. The diphosphorylated derivatives of Adefovir targeted the viral DNA polymerase and also acted as DNA chain terminators. Adenylate kinase is shown to be responsible for the first phosphorylation, which was followed by ADP kinase and creatine kinase, forming Adefovir diphosphate[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Unaffected by food, Adefovir achieves 60% oral bioavailability. Its half-life is 12-30 hours and Adefovir undergoes renal excretion without significant metabolites. Adefovir does not substantially affect the cytochrome P450 system[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

273.19

Formula

C8H12N5O4P

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=C2N=CN(CCOCP(O)(O)=O)C2=NC=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

0.1 M NaOH : 10 mg/mL (36.60 mM; ultrasonic and adjust pH to 10 with NaOH)

H2O : < 0.1 mg/mL (insoluble)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6605 mL 18.3023 mL 36.6046 mL
5 mM 0.7321 mL 3.6605 mL 7.3209 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.74%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
0.1 M NaOH 1 mM 3.6605 mL 18.3023 mL 36.6046 mL 91.5114 mL
5 mM 0.7321 mL 3.6605 mL 7.3209 mL 18.3023 mL
10 mM 0.3660 mL 1.8302 mL 3.6605 mL 9.1511 mL
15 mM 0.2440 mL 1.2202 mL 2.4403 mL 6.1008 mL
20 mM 0.1830 mL 0.9151 mL 1.8302 mL 4.5756 mL
25 mM 0.1464 mL 0.7321 mL 1.4642 mL 3.6605 mL
30 mM 0.1220 mL 0.6101 mL 1.2202 mL 3.0504 mL
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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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