alpha-Mangostin (Synonyms: α-Mangostin)

Name: alpha-Mangostin
Brand: MedChemExpress
SKU: HY-N0328
Rating: 5.0 (6 reviews)

Cat. No.: HY-N0328 Purity: 99.64%: FAQs
Data Sheet SDS COA Handling Instructions

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alpha-Mangostin (α-Mangostin) is a dietary xanthone with broad biological activities, such as antioxidant, anti-allergic, antiviral, antibacterial, anti-inflammatory and anticancer effects. It is an inhibitor of mutant IDH1 (IDH1-R132H) with a K_i of 2.85 μM.

For research use only. We do not sell to patients.

alpha-Mangostin Chemical Structure

CAS No. : 6147-11-1

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of alpha-Mangostin:

4 Publications Citing Use of MCE alpha-Mangostin

: alpha-Mangostin purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jan;24(1):760-771. [Abstract]; MA (alpha-Mangostin) impedes proliferation and induces cell cycle arrest in gallbladder cancer cells. GBC-SD cells are treated with MA, and proliferation was assessed by the EdU assay.

: alpha-Mangostin purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jan;24(1):760-771. [Abstract]; MA (alpha-Mangostin) impedes proliferation and induces cell cycle arrest in gallbladder cancer cells. NOZ cells are treated with MA, and proliferation was assessed by the EdU assay.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 2.85 μM (IDH1-R132H)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	2.7 μM Compound: SST0673	Antiproliferative activity against human A375 cells assessed as reduction in cell viability incubated for 72 hrs by trypan blue or crystal violet staining based assay Antiproliferative activity against human A375 cells assessed as reduction in cell viability incubated for 72 hrs by trypan blue or crystal violet staining based assay	[PMID: 32435392]
A-431	IC₅₀	7.2 μM Compound: NSC27593	Antiproliferative activity against human A-431 cells overexpressing EGFR assessed as cell growth inhibition by MTT assay Antiproliferative activity against human A-431 cells overexpressing EGFR assessed as cell growth inhibition by MTT assay	[PMID: 34995690]
ASPC1	IC₅₀	4.02 μM Compound: 1	Cytotoxicity against human AsPC1 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay Cytotoxicity against human AsPC1 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay	[PMID: 24717154]
B16-F10	IC₅₀	3.23 μM Compound: 1	Cytotoxicity against mouse B16F10 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay Cytotoxicity against mouse B16F10 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay	[PMID: 24717154]
Daoy	IC₅₀	1.9 μM Compound: SST0673	Antiproliferative activity against human DaOY cells assessed as reduction in cell viability incubated for 72 hrs by trypan blue or crystal violet staining based assay Antiproliferative activity against human DaOY cells assessed as reduction in cell viability incubated for 72 hrs by trypan blue or crystal violet staining based assay	[PMID: 32435392]
DLD-1	IC₅₀	7.5 μM Compound: alpha-mangostin	Cytotoxicity against human DLD1 cells after 24 hrs by Trypan blue exclusion test Cytotoxicity against human DLD1 cells after 24 hrs by Trypan blue exclusion test	[PMID: 17553685]
HeLa	IC₅₀	> 10 μM Compound: 3	Inhibition of NFkappa p65 in nuclear extract of human HeLa cells assessed as blockade of NFkappa p65 binding to biotinylated-consesus sequence by ELISA Inhibition of NFkappa p65 in nuclear extract of human HeLa cells assessed as blockade of NFkappa p65 binding to biotinylated-consesus sequence by ELISA	[PMID: 21428375]
HeLa	IC₅₀	11.95 μM Compound: NSC27593	Antiproliferative activity against human HeLa cells assessed as cell growth inhibition by MTT assay Antiproliferative activity against human HeLa cells assessed as cell growth inhibition by MTT assay	[PMID: 34995690]
HeLa S3	IC₅₀	> 10 μM Compound: 12	Cytotoxicity in human HeLaS3 cells incubated for 72 hrs by MTT assay Cytotoxicity in human HeLaS3 cells incubated for 72 hrs by MTT assay	[PMID: 30978023]
HepG2	IC₅₀	> 10 μM Compound: 12	Cytotoxicity in human HepG2 cells incubated for 72 hrs by MTT assay Cytotoxicity in human HepG2 cells incubated for 72 hrs by MTT assay	[PMID: 30978023]
HepG2	IC₅₀	8.55 μM Compound: alpha-Mangostin	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31343173]
HL-60	IC₅₀	6.8 μM Compound: 1	Cytotoxicity against human HL60 cells assessed as cell viability after 72 hrs by Trypan blue exclusion test Cytotoxicity against human HL60 cells assessed as cell viability after 72 hrs by Trypan blue exclusion test	[PMID: 12932141]
HT-29	IC₅₀	> 10 μM Compound: 12	Cytotoxicity in human HT-29 cells incubated for 72 hrs by MTT assay Cytotoxicity in human HT-29 cells incubated for 72 hrs by MTT assay	[PMID: 30978023]
HT-29	IC₅₀	0.2 μM Compound: 3	Effect on mitochondrial membrane potential in human HT-29 cells after 2 hrs using JC-1 staining by fluorescence assay Effect on mitochondrial membrane potential in human HT-29 cells after 2 hrs using JC-1 staining by fluorescence assay	[PMID: 21428375]
HT-29	ED₅₀	4.1 μM Compound: 3	Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay	[PMID: 21428375]
KB	IC₅₀	> 10 μM Compound: 12	Cytotoxicity in human KB cells incubated for 72 hrs by MTT assay Cytotoxicity in human KB cells incubated for 72 hrs by MTT assay	[PMID: 30978023]
KB	IC₅₀	14.14 μM Compound: alpha-mangostin	Cytotoxicity against human KB cells after 48 hrs by MTT assay Cytotoxicity against human KB cells after 48 hrs by MTT assay	[PMID: 30684866]
MCF7	IC₅₀	> 10 μM Compound: 12	Cytotoxicity in human MCF7 cells incubated for 72 hrs by MTT assay Cytotoxicity in human MCF7 cells incubated for 72 hrs by MTT assay	[PMID: 30978023]
MCF7	IC₅₀	3.5 μg/mL Compound: 6	Cytotoxicity against human MCF7 cells assessed as [3H]hypoxanthin incorporation after 24 to 72 hrs Cytotoxicity against human MCF7 cells assessed as [3H]hypoxanthin incorporation after 24 to 72 hrs	[PMID: 19296616]
MCF7	IC₅₀	9.95 μM Compound: alpha-Mangostin	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 31343173]
MDA-MB-231	IC₅₀	12.75 μM Compound: 105	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33619958]
MDA-MB-231	IC₅₀	3.04 μM Compound: 1	Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay	[PMID: 24717154]
MRC5	CC₅₀	7.5 μM Compound: alpha-mangostin	Cytotoxicity against human MRC5 SV2 cells measured after 72 hrs by resazurin based fluorescence assay Cytotoxicity against human MRC5 SV2 cells measured after 72 hrs by resazurin based fluorescence assay	[PMID: 27517813]
NCI-H460	IC₅₀	3.23 μM Compound: 1	Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay	[PMID: 24717154]
SW-620	IC₅₀	2.97 μM Compound: 1	Cytotoxicity against human SW620 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay Cytotoxicity against human SW620 cells assessed as inhibition of cell proliferation after 48 hrs by XTT assay	[PMID: 24717154]

In Vitro

alpha-Mangostin (α-Mangostin) exhibits a selective inhibitory effect on IDH1-R132H, but not on IDH1. alpha-Mangostin (α-Mangostin) competitively inhibits the binding of alpha-mangostin (α-KG) to IDH1-R132H. The structure–relationship study reveals that alpha-Mangostin (α-Mangostin) exhibits the strongest core inhibitor structure. alpha-Mangostin (α-Mangostin) selectively promotes demethylation of 5-methylcytosine (5mC) and histone H3 trimethylated lysine residues in IDH1 (+/R132H) MCF10A cells^[1]. Cell proliferation significantly decreases in a dose-dependent manner in the cells treated with alpha-mangostin. Alpha-mangostin also increases the levels of Bax (pro-apoptotic), cleaved caspase-3, cleaved caspase-9 and cleaved-poly(ADP-ribose) polymerase (PARP)^[2]. alpha-Mangostin (α-Mangostin) significantly inhibits light-induced degeneration of photoreceptors and 200 μM H₂O₂-induced apoptosis of RPE cells. 200 μM H₂O₂-induced generation of reactive oxygen species (ROS) and light-induced generation of malondialdehyde (MDA) are suppressed by alpha-Mangostin (α-Mangostin)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

alpha-Mangostin (α-Mangostin) reduces risk of liver fibrosis through the decrease in p53 expression as compared to the TAA_DMSO treatment. The serum levels of the liver enzymes AST and ALT after treatment with α-mangostin decrease as compared to DMSO alone^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

410.46

Formula

C₂₄H₂₆O₆

CAS No.

6147-11-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2=C(OC3=C1C(C/C=C(C)\C)=C(OC)C(O)=C3)C=C(O)C(C/C=C(C)\C)=C2O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 110 mg/mL (267.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4363 mL	12.1815 mL	24.3629 mL
5 mM	0.4873 mL	2.4363 mL	4.8726 mL
10 mM	0.2436 mL	1.2181 mL	2.4363 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.75 mg/mL (6.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (27.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (278 KB) SDS (479 KB)

COA (249 KB) LCMS (255 KB)

Handling Instructions (2659 KB)

References

[1]. Kim HJ, et al. Discovery of α-mangostin as a novel competitive inhibitor against mutant isocitrate dehydrogenase-1. Bioorg Med Chem Lett. 2015 Dec 1;25(23):5625-31. [Content Brief]

[2]. Lee HN, et al. Antitumor and apoptosis-inducing effects of α-mangostin extracted from the pericarp of the mangosteen fruit (Garcinia mangostana L.) in YD-15 tongue mucoepidermoid carcinoma cells. Int J Mol Med. 2016 Apr;37(4):939-48. [Content Brief]

Cell Assay ^[1]	IDH1^+/+ and IDH1 MCF10A cells are grown in DMEM/F-12 media, supplemented with 5% horse serum, 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 10 μg/mL insulin. IDH1^+/+ and IDH1 MCF10A cells are seeded in 6 well plates. After an exposure to 5 μM alpha-mangostin. cells are collected after indicated times and the viable cell number is calculated, using hemacytometer counting^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[4]	Rats: Male Wistar rats are divided into 3 groups and treated with intraperitoneal injections of TAA (200 mg/kg). One subgroup is left untreated whereas the other two are treated either with 100 mg/kg alpha-mangostin or vehicle alone (80% DMSO, 20% water), which are administered intraperitoneally 3 times per weekfor a total of4 weeks. The incidence offibrotic nodules on the liver and the serum levels of the liver enzymes aspartate transaminase (AST) and alanine transaminase (ALT) are measured^[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Kim HJ, et al. Discovery of α-mangostin as a novel competitive inhibitor against mutant isocitrate dehydrogenase-1. Bioorg Med Chem Lett. 2015 Dec 1;25(23):5625-31. [Content Brief] [2]. Lee HN, et al. Antitumor and apoptosis-inducing effects of α-mangostin extracted from the pericarp of the mangosteen fruit (Garcinia mangostana L.) in YD-15 tongue mucoepidermoid carcinoma cells. Int J Mol Med. 2016 Apr;37(4):939-48. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4363 mL	12.1815 mL	24.3629 mL	60.9073 mL
	5 mM	0.4873 mL	2.4363 mL	4.8726 mL	12.1815 mL
	10 mM	0.2436 mL	1.2181 mL	2.4363 mL	6.0907 mL
	15 mM	0.1624 mL	0.8121 mL	1.6242 mL	4.0605 mL
	20 mM	0.1218 mL	0.6091 mL	1.2181 mL	3.0454 mL
	25 mM	0.0975 mL	0.4873 mL	0.9745 mL	2.4363 mL
	30 mM	0.0812 mL	0.4060 mL	0.8121 mL	2.0302 mL
	40 mM	0.0609 mL	0.3045 mL	0.6091 mL	1.5227 mL
	50 mM	0.0487 mL	0.2436 mL	0.4873 mL	1.2181 mL
	60 mM	0.0406 mL	0.2030 mL	0.4060 mL	1.0151 mL
	80 mM	0.0305 mL	0.1523 mL	0.3045 mL	0.7613 mL
	100 mM	0.0244 mL	0.1218 mL	0.2436 mL	0.6091 mL

alpha-Mangostin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

alpha-Mangostin6147-11-1α-MangostinReactive Oxygen SpeciesApoptosisBacterialFungalVirus ProteaseInhibitorinhibitorinhibit

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alpha-Mangostin (Synonyms: α-Mangostin)

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Other Forms of alpha-Mangostin:

alpha-Mangostin Related Antibodies

4 Publications Citing Use of MCE alpha-Mangostin

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Purity & Documentation

References

Customer Review

alpha-Mangostin Related Antibodies

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Complete Stock Solution Preparation Table

alpha-Mangostin Related Classifications

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