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  4. Anhydrous sodium dihydrogen phosphate, for cell culture

Anhydrous sodium dihydrogen phosphate, for cell culture  (Synonyms: Sodium phosphate monobasic, for cell culture; Monosodium dihydrogen orthophosphate, for cell culture; Monosodium phosphate, for cell culture)

Cat. No.: HY-B2243D
Data Sheet
SDS
COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

Help & FAQs

Anhydrous sodium dihydrogen phosphate, for cell culture is an inorganic salt compound that can be used as a buffer and nutritional supplement. Anhydrous sodium dihydrogen phosphate, for cell culture can be used in cell culture.

For research use only. We do not sell to patients.

Anhydrous sodium dihydrogen phosphate, for cell culture Chemical Structure

Anhydrous sodium dihydrogen phosphate, for cell culture Chemical Structure

CAS No. : 7558-80-7

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE Anhydrous sodium dihydrogen phosphate, for cell culture

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Anhydrous sodium dihydrogen phosphate, for cell culture is an inorganic salt compound that can be used as a buffer and nutritional supplement. Anhydrous sodium dihydrogen phosphate, for cell culture can be used in cell culture[1].

In Vitro

Dihydrogen monosodium phosphate (NaH2PO4, NDHP) is a chemical compound of sodium with a phosphate counter ion. Sorbitan monooleate, in combination with other sodium phosphates, can serve a pH buffer. Dihydrogen monosodium phosphate can be used as an excipient, such as buffer, chelating agent. Pharmaceutical excipients, or pharmaceutical auxiliaries, refer to other chemical substances used in the pharmaceutical process other than pharmaceutical ingredients. Pharmaceutical excipients generally refer to inactive ingredients in pharmaceutical preparations, which can improve the stability, solubility and processability of pharmaceutical preparations. Pharmaceutical excipients also affect the absorption, distribution, metabolism, and elimination (ADME) processes of co-administered drugs[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

119.98

Formula

NaH2PO4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=P(O[O])=O.[NaH2]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL (833.47 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 8.3347 mL 41.6736 mL 83.3472 mL
5 mM 1.6669 mL 8.3347 mL 16.6694 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation

Data Sheet (270 KB) SDS (393 KB)

COA (244 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 8.3347 mL 41.6736 mL 83.3472 mL 208.3681 mL
5 mM 1.6669 mL 8.3347 mL 16.6694 mL 41.6736 mL
10 mM 0.8335 mL 4.1674 mL 8.3347 mL 20.8368 mL
15 mM 0.5556 mL 2.7782 mL 5.5565 mL 13.8912 mL
20 mM 0.4167 mL 2.0837 mL 4.1674 mL 10.4184 mL
25 mM 0.3334 mL 1.6669 mL 3.3339 mL 8.3347 mL
30 mM 0.2778 mL 1.3891 mL 2.7782 mL 6.9456 mL
40 mM 0.2084 mL 1.0418 mL 2.0837 mL 5.2092 mL
50 mM 0.1667 mL 0.8335 mL 1.6669 mL 4.1674 mL
60 mM 0.1389 mL 0.6946 mL 1.3891 mL 3.4728 mL
80 mM 0.1042 mL 0.5209 mL 1.0418 mL 2.6046 mL
100 mM 0.0833 mL 0.4167 mL 0.8335 mL 2.0837 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Anhydrous sodium dihydrogen phosphate, for cell culture Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Keywords:

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