Anti-inflammatory agent 49

Name: Anti-inflammatory agent 49
Brand: MedChemExpress
SKU: HY-155656
Rating: 4.5 (1 reviews)

Cat. No.: HY-155656 Purity: 98.26%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

nti-inflammatory agent 49 (compound SC9) is a quite potent and selective inhibitor of Drp1-Fis1 interaction and can reduce FIS1-mediated mitochondrial dysfunction. The IC₅₀ of SC9 inhibiting GTPase in vitro is 270 nM.

For research use only. We do not sell to patients.

Anti-inflammatory agent 49 Chemical Structure

CAS No. : 851471-44-8

Size	Price	Stock	Quantity
5 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 840	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1350	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC50: 270 nM (dynamin-related protein 1 ,Drp1 )^[1]

In Vitro

1.Anti-inflammatory agent 49 can reduce the mitochondrial dysfunction of H9c2 cells induced by LPS (HY-D1056) and save the mice endotoxemia induced by LPS^[1].
2.Anti-inflammatory agent 49 inhibits Drp1 association with the mitochondria and Drp1-Fis1 interaction following LPS treatment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	H9c2 cells^[1]
Concentration:	2 µM
Incubation Time:	16 h
Result:	Decreased the percentage of cells with fragmented mitochondria. The percent of fragmented cells was decreased from 22% (LPS + Veh) to 9% (LPS + SC9), relative to 4% in the absence of LPS^[1].

Western Blot Analysis

Cell Line:	H9c2 cells^[1]
Concentration:	2 µM
Incubation Time:	24 h
Result:	Decreased the number of high Drp1-Fis1 cells from 67% (LPS + Veh) to 28% (LPS + P110) and to 14% (LPS + SC9).^[1]

In Vivo

Anti-inflammatory agent 49 (10 mg/kg, every 8 h for 72 h) rescues mice from LPS-induced endotoxemia^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c AnNCrl micee (Strain Code 028)^[1]
Dosage:	LPS doses 10–16.67 mg/kg, SC9 doses 10 mg/kg(after 4 h)
Administration:	Intraperitoneal injection: 0.2 mL of LPS,0.1 mL of CS9; scored every 8 h for 72 h.
Result:	Improved mouse survival at all LPS doses tested at the different LPS doses (10 to 16.6 mg/kg) . Dramatically reduced the occurrence of critical symptoms such as respiratory distress.

Molecular Weight

466.52

Formula

C₂₁H₂₂N₈O₃S

CAS No.

851471-44-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1NC(N(CC2=CC=CC=C2)C(N)=C1N(C)C(CSC3=NN4C(C)=CC(C)=NC4=N3)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (214.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1435 mL	10.7177 mL	21.4353 mL
5 mM	0.4287 mL	2.1435 mL	4.2871 mL
10 mM	0.2144 mL	1.0718 mL	2.1435 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.26%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (249 KB) HNMR (156 KB) RP-HPLC (219 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. Luis Rios, et al. Targeting an allosteric site in dynaminrelated protein 1 to inhibit Fis1-mediated mitochondrial dysfunction. Nat Commun. 2023 Jul 19;14(1):4356 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1435 mL	10.7177 mL	21.4353 mL	53.5883 mL
	5 mM	0.4287 mL	2.1435 mL	4.2871 mL	10.7177 mL
	10 mM	0.2144 mL	1.0718 mL	2.1435 mL	5.3588 mL
	15 mM	0.1429 mL	0.7145 mL	1.4290 mL	3.5726 mL
	20 mM	0.1072 mL	0.5359 mL	1.0718 mL	2.6794 mL
	25 mM	0.0857 mL	0.4287 mL	0.8574 mL	2.1435 mL
	30 mM	0.0715 mL	0.3573 mL	0.7145 mL	1.7863 mL
	40 mM	0.0536 mL	0.2679 mL	0.5359 mL	1.3397 mL
	50 mM	0.0429 mL	0.2144 mL	0.4287 mL	1.0718 mL
	60 mM	0.0357 mL	0.1786 mL	0.3573 mL	0.8931 mL
	80 mM	0.0268 mL	0.1340 mL	0.2679 mL	0.6699 mL
	100 mM	0.0214 mL	0.1072 mL	0.2144 mL	0.5359 mL