Antibacterial agent 125

Name: Antibacterial agent 125
Brand: MedChemExpress
SKU: HY-151918
Rating: 4.5 (1 reviews)

Cat. No.: HY-151918 Purity: 98.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Antibacterial agent 125 is an antibacterial agent. Antibacterial agent 125 has a potent antimicrobial activity against clinically relevant Gram-positive pathogens with MIC₅₀ values range from 0.25 - 8 μM. Antibacterial agent 125 can be used for the research of antimicrobial resistance.

For research use only. We do not sell to patients.

Antibacterial agent 125 Chemical Structure

CAS No. : 1274611-43-6

Size	Price	Stock	Quantity
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

MIC50: 0.25 - 8 μM (Gram-positive pathogens)^[1]

In Vitro

Antibacterial agent 125 has activity against clinically relevant Gram-positive pathogens with MIC₅₀ values from 0.25-8 μM^[1].
Antibacterial agent 125 has inhibitory activity against methicillin-resistant Staphylococcus aureus strain without showing cytotoxicity in human cells^[1].
Antibacterial agent 125 has high metabolic stability and excellent safety profile^[1].
Antibacterial agent 125 not induces resistance after ten passages and has a moderate ability to inhibit the biofilm formation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

270.71

Formula

C₁₅H₁₁ClN₂O

CAS No.

1274611-43-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C(C#N)C=C1)CC2=CC(Cl)=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (923.50 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6940 mL	18.4699 mL	36.9399 mL
5 mM	0.7388 mL	3.6940 mL	7.3880 mL
10 mM	0.3694 mL	1.8470 mL	3.6940 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.90%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (244 KB) HNMR (233 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Francesca Brunelli, et al. Isocyanides in med chem: A scaffold hopping approach for the identification of novel 4-isocyanophenylamides as potent antibacterial agents against methicillin-resistant Staphylococcusaureus. Eur J Med Chem. 2022 Nov 26;246:114950. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6940 mL	18.4699 mL	36.9399 mL	92.3497 mL
	5 mM	0.7388 mL	3.6940 mL	7.3880 mL	18.4699 mL
	10 mM	0.3694 mL	1.8470 mL	3.6940 mL	9.2350 mL
	15 mM	0.2463 mL	1.2313 mL	2.4627 mL	6.1566 mL
	20 mM	0.1847 mL	0.9235 mL	1.8470 mL	4.6175 mL
	25 mM	0.1478 mL	0.7388 mL	1.4776 mL	3.6940 mL
	30 mM	0.1231 mL	0.6157 mL	1.2313 mL	3.0783 mL
	40 mM	0.0923 mL	0.4617 mL	0.9235 mL	2.3087 mL
	50 mM	0.0739 mL	0.3694 mL	0.7388 mL	1.8470 mL
	60 mM	0.0616 mL	0.3078 mL	0.6157 mL	1.5392 mL
	80 mM	0.0462 mL	0.2309 mL	0.4617 mL	1.1544 mL
	100 mM	0.0369 mL	0.1847 mL	0.3694 mL	0.9235 mL