Antiviral agent 24

Name: Antiviral agent 24
Brand: MedChemExpress
SKU: HY-151926
Rating: 4.5 (1 reviews)

Cat. No.: HY-151926 Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Antiviral agent 24 is a potent antiviral agent with EC₅₀ values of 0.101, 19.9, 91.2 µM for EV71, CVA21, EV68, respectively. Antiviral agent 24 inhibits METTL3/METTL14 activity in a dose-dependent manner.

For research use only. We do not sell to patients.

Antiviral agent 24 Chemical Structure

CAS No. : 23661-03-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 275	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 275	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 400	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1280	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
G-361	IC₅₀	> 166.7 μM Compound: 44	Antitumor activity against human G361 cells after 72 hrs by Calcein AM assay Antitumor activity against human G361 cells after 72 hrs by Calcein AM assay	[PMID: 17418578]
HOS	IC₅₀	> 166.7 μM Compound: 44	Antitumor activity against human HOS cells after 72 hrs by Calcein AM assay Antitumor activity against human HOS cells after 72 hrs by Calcein AM assay	[PMID: 17418578]
K562	IC₅₀	> 166.7 μM Compound: 44	Antitumor activity against human K562 cells after 72 hrs by Calcein AM assay Antitumor activity against human K562 cells after 72 hrs by Calcein AM assay	[PMID: 17418578]

In Vitro

Antiviral agent 24 (compound 25) (0-1000 µM) inhibits METTL3/METTL14 complex activity with an EC₅₀ value of 129 µM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

425.36

Formula

C₁₈H₁₈F₃N₅O₄

CAS No.

23661-03-2

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(N=CN=C3NCC4=CC=CC(C(F)(F)F)=C4)=C3N=C2)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (587.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3509 mL	11.7547 mL	23.5095 mL
5 mM	0.4702 mL	2.3510 mL	4.7019 mL
10 mM	0.2351 mL	1.1755 mL	2.3510 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (253 KB) HNMR (262 KB) LCMS (253 KB)

Handling Instructions (2659 KB)

References

[1]. Salerno M, et al. Rational design of novel nucleoside analogues reveals potent antiviral agents for EV71. Eur J Med Chem. 2022 Nov 24;246:114942. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3509 mL	11.7547 mL	23.5095 mL	58.7737 mL
	5 mM	0.4702 mL	2.3509 mL	4.7019 mL	11.7547 mL
	10 mM	0.2351 mL	1.1755 mL	2.3509 mL	5.8774 mL
	15 mM	0.1567 mL	0.7836 mL	1.5673 mL	3.9182 mL
	20 mM	0.1175 mL	0.5877 mL	1.1755 mL	2.9387 mL
	25 mM	0.0940 mL	0.4702 mL	0.9404 mL	2.3509 mL
	30 mM	0.0784 mL	0.3918 mL	0.7836 mL	1.9591 mL
	40 mM	0.0588 mL	0.2939 mL	0.5877 mL	1.4693 mL
	50 mM	0.0470 mL	0.2351 mL	0.4702 mL	1.1755 mL
	60 mM	0.0392 mL	0.1959 mL	0.3918 mL	0.9796 mL
	80 mM	0.0294 mL	0.1469 mL	0.2939 mL	0.7347 mL
	100 mM	0.0235 mL	0.1175 mL	0.2351 mL	0.5877 mL