ATG7-IN-3

Name: ATG7-IN-3
Brand: MedChemExpress
SKU: HY-146131
Rating: 4.5 (1 reviews)

Cat. No.: HY-146131: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ATG7-IN-3 (compound 18) is a potent ATG7 inhibitor, with an IC₅₀ of 0.048 μM. ATG7-IN-3 inhibits autophagy. ATG7-IN-3 inhibits the formation of endogenous LC3B puncta in the neuroglioma cell line H4.

For research use only. We do not sell to patients.

ATG7-IN-3 Chemical Structure

CAS No. : 2226229-76-9

Size	Price	Stock	Quantity
1 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 2350	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC₅₀: 0.048 ± 0.022 μM (ATG7), 0.280 ± 0.051 μM (Thioester), 0.938 ± 0.231 μM (LC3B)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
NCI-H1650	EC₅₀	10.4 μM Compound: 18	Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay	[PMID: 32912429]
SK-OV-3	EC₅₀	> 20 μM Compound: 18	Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay	[PMID: 32912429]
SK-OV-3	EC₅₀	3 μM Compound: 18	Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay	[PMID: 32912429]

Molecular Weight

376.41

Formula

C₁₁H₁₆N₆O₅S₂

CAS No.

2226229-76-9

Appearance

Solid

Color

White to off-white

SMILES

CSC1=NN([C@]2([H])O[C@@H]([C@H](C2)O)COS(N)(=O)=O)C3=NC=NC(N)=C31

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (265.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6567 mL	13.2834 mL	26.5668 mL
5 mM	0.5313 mL	2.6567 mL	5.3134 mL
10 mM	0.2657 mL	1.3283 mL	2.6567 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.26%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (254 KB) HNMR (144 KB) RP-HPLC (167 KB) LCMS (385 KB)

Handling Instructions (2659 KB)

References

[1]. Huang SC, et al. Discovery and optimization of pyrazolopyrimidine sulfamates as ATG7 inhibitors. Bioorg Med Chem. 2020 Oct 1;28(19):115681. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6567 mL	13.2834 mL	26.5668 mL	66.4169 mL
	5 mM	0.5313 mL	2.6567 mL	5.3134 mL	13.2834 mL
	10 mM	0.2657 mL	1.3283 mL	2.6567 mL	6.6417 mL
	15 mM	0.1771 mL	0.8856 mL	1.7711 mL	4.4278 mL
	20 mM	0.1328 mL	0.6642 mL	1.3283 mL	3.3208 mL
	25 mM	0.1063 mL	0.5313 mL	1.0627 mL	2.6567 mL
	30 mM	0.0886 mL	0.4428 mL	0.8856 mL	2.2139 mL
	40 mM	0.0664 mL	0.3321 mL	0.6642 mL	1.6604 mL
	50 mM	0.0531 mL	0.2657 mL	0.5313 mL	1.3283 mL
	60 mM	0.0443 mL	0.2214 mL	0.4428 mL	1.1069 mL
	80 mM	0.0332 mL	0.1660 mL	0.3321 mL	0.8302 mL
	100 mM	0.0266 mL	0.1328 mL	0.2657 mL	0.6642 mL

ATG7-IN-3 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ATG7-IN-32226229-76-9Atg8/LC3Atg7AutophagyAutophagy-related 8| E1 enzymeautophagyH1650 cellsH4 cellsneurogliomaLC3BNBR1Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

ATG7-IN-3

Customer Review

ATG7-IN-3 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ATG7-IN-3 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report