Beta-Acetoxyisovalerylshikonin

Name: Beta-Acetoxyisovalerylshikonin
Brand: MedChemExpress
SKU: HY-N2188
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2188 Purity: 99.62%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Beta-Acetoxyisovalerylshikonin is a naphthoquinone derivative isolated from Arnebia euchroma.

For research use only. We do not sell to patients.

Beta-Acetoxyisovalerylshikonin Chemical Structure

CAS No. : 69091-17-4

Size	Price	Stock	Quantity
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 185	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 335	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Beta-Acetoxyisovalerylshikonin

•Cell Mol Biol Lett. 2021 Jul 31;26(1):35. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Beta-Acetoxyisovalerylshikonin is a naphthoquinone derivative isolated from Arnebia euchroma^[1]^[2].

Molecular Weight

430.45

Formula

C₂₃H₂₆O₈

CAS No.

69091-17-4

Appearance

Solid

Color

Brown to black

SMILES

CC(C)(OC(C)=O)CC(O[C@H](C(C1=O)=CC(C2=C1C(O)=CC=C2O)=O)C/C=C(C)\C)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (116.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3232 mL	11.6158 mL	23.2315 mL
5 mM	0.4646 mL	2.3232 mL	4.6463 mL
10 mM	0.2323 mL	1.1616 mL	2.3232 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.81 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (253 KB) HNMR (283 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Sharma N, et al. Simultaneous densitometric determination of shikonin, acetylshikonin, and beta-acetoxyisovaleryl-shikonin in ultrasonic-assisted extracts of four Arnebia species using reversed-phase thin layer chromatography. J Sep Sci. 2009 Sep;32(18):3239-45. [Content Brief]

[2]. Sharma N, et al. Isolation and purification of acetylshikonin and beta-acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC. J Sep Sci. 2008 Mar;31(4):629-35. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3232 mL	11.6158 mL	23.2315 mL	58.0788 mL
	5 mM	0.4646 mL	2.3232 mL	4.6463 mL	11.6158 mL
	10 mM	0.2323 mL	1.1616 mL	2.3232 mL	5.8079 mL
	15 mM	0.1549 mL	0.7744 mL	1.5488 mL	3.8719 mL
	20 mM	0.1162 mL	0.5808 mL	1.1616 mL	2.9039 mL
	25 mM	0.0929 mL	0.4646 mL	0.9293 mL	2.3232 mL
	30 mM	0.0774 mL	0.3872 mL	0.7744 mL	1.9360 mL
	40 mM	0.0581 mL	0.2904 mL	0.5808 mL	1.4520 mL
	50 mM	0.0465 mL	0.2323 mL	0.4646 mL	1.1616 mL
	60 mM	0.0387 mL	0.1936 mL	0.3872 mL	0.9680 mL
	80 mM	0.0290 mL	0.1452 mL	0.2904 mL	0.7260 mL
	100 mM	0.0232 mL	0.1162 mL	0.2323 mL	0.5808 mL