BMS-199264 hydrochloride

Name: BMS-199264 hydrochloride
Brand: MedChemExpress
SKU: HY-118960
Rating: 4.5 (1 reviews)

Cat. No.: HY-118960 Purity: 99.28%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

BMS-199264 hydrochloride is an inhibitor of F1F0 ATP hydrolase (IC₅₀=0.5 μM) without inhibitory effect on F1F0 ATP synthase. BMS-199264 hydrochloride selectively inhibits ATP decline during ischemia to reduces cardiac necrosis. BMS-199264 hydrochloride also enhances the recovery of contractile function following reperfusion.

For research use only. We do not sell to patients.

BMS-199264 hydrochloride Chemical Structure

CAS No. : 186180-83-6

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

In Vitro

BMS-199264 hydrochloride (1 μM, 3 μM, 10 μM) increases the time to onset of contracture and decreases LDH release, in a concentration-dependent manner in isolated rat hearts after a 25-min global ischemia followed by a 30-min reperfusion^[1].
BMS-199264 hydrochloride (3 μM) shows different effects on ATP synthase or hydrolase activity with values of 0.23 μM ATP/min/mg and 0.18 μM ATP/min/mg, respecctively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

567.53

Formula

C₂₆H₃₂Cl₂N₄O₄S

CAS No.

186180-83-6

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1C(C)(OC2=CC=C(C=C2[C@H]1N(CC3=NC=CN3)C4=CC=C(C=C4)Cl)S(=O)(N5CCCCC5)=O)C.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (176.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.7620 mL	8.8101 mL	17.6202 mL
5 mM	0.3524 mL	1.7620 mL	3.5240 mL
10 mM	0.1762 mL	0.8810 mL	1.7620 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.28%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (256 KB) HNMR (281 KB) RP-HPLC (168 KB) LCMS (105 KB)

Handling Instructions (2659 KB)

References

[1]. Grover GJ, et al. Pharmacological profile of the selective mitochondrial F1F0 ATP hydrolase inhibitor BMS-199264 in myocardial ischemia. Cardiovasc Ther. 2008 Winter;26(4):287-96. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.7620 mL	8.8101 mL	17.6202 mL	44.0505 mL
	5 mM	0.3524 mL	1.7620 mL	3.5240 mL	8.8101 mL
	10 mM	0.1762 mL	0.8810 mL	1.7620 mL	4.4051 mL
	15 mM	0.1175 mL	0.5873 mL	1.1747 mL	2.9367 mL
	20 mM	0.0881 mL	0.4405 mL	0.8810 mL	2.2025 mL
	25 mM	0.0705 mL	0.3524 mL	0.7048 mL	1.7620 mL
	30 mM	0.0587 mL	0.2937 mL	0.5873 mL	1.4684 mL
	40 mM	0.0441 mL	0.2203 mL	0.4405 mL	1.1013 mL
	50 mM	0.0352 mL	0.1762 mL	0.3524 mL	0.8810 mL
	60 mM	0.0294 mL	0.1468 mL	0.2937 mL	0.7342 mL
	80 mM	0.0220 mL	0.1101 mL	0.2203 mL	0.5506 mL
	100 mM	0.0176 mL	0.0881 mL	0.1762 mL	0.4405 mL