Brilliant blue G-250

Name: Brilliant blue G-250
Brand: MedChemExpress
SKU: HY-D0014
Rating: 5.0 (3 reviews)

Cat. No.: HY-D0014 Purity: ≥95.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome.

For research use only. We do not sell to patients.

Brilliant blue G-250 Chemical Structure

CAS No. : 6104-58-1

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
100 mg	USD 65	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Brilliant blue G-250

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Purity & Documentation
References
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Description

In Vitro

Staining Proteins in Polyacrylamide Gel Electrophoresis with Brilliant Blue G-250^[1]
1. Solution Preparation
Coomassie staining solution:
(1) Dissolve 100 g aluminum sulfate (14-18 hydrate) in 2000 mL Milli-Q purified water. Add 200 mL ethanol (96%) and mix well.
(2) Add 0.4 g Brilliant Blue G-250 and stir until dissolved.
(3) Slowly add 47 mL phosphoric acid (85%) while stirring.
(4) Finally, adjust the volume to 2000 mL with Milli-Q purified water.
Note: Do not filter the solution. It should appear colloidal with suspended particles.
Destaining solution:
Prepare 2000 mL Milli-Q purified water, adding 200 mL ethanol (96%) and 47 mL phosphoric acid (85%).
2. Staining Procedure
(1) After protein electrophoresis, carefully remove the gel from the glass plates. Wash the gel with Milli-Q purified water 3 times for 10 minutes each to remove SDS. Shake the Coomassie staining solution before use to disperse the colloidal particles. Immerse the gel in the staining solution and agitate on a shaker for 2-12 hours.
(2) Protein spots will begin to appear after 10 minutes, and 80% of staining will be completed within 2 hours. For optimal results, overnight staining is recommended.
(3) After staining, remove the Coomassie solution and rinse the gel twice with Milli-Q purified water. Place the gel in the destaining solution and agitate for 10-60 minutes to remove excess dye.
3. Rinsing and Storage
Rinse the gel twice with Milli-Q purified water to restore its original thickness. The gel can be stored in a refrigerator with an acidic solution to prevent mold contamination.
4. Notes
(1) Ensure the gel is thoroughly washed before staining, as residual SDS will interfere with dye-protein binding.
(2) The staining solution can be reused as long as particles remain.
(3) Store the staining solution in a dark bottle to extend its shelf life.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

854.02

Formula

C₄₇H₄₈N₃NaO₇S₂

CAS No.

6104-58-1

Appearance

Solid

Color

Dark purple to black

SMILES

O=S(C1=CC(CN(C2=CC(C)=C(/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C(C)=C/C(C=C/5)=[N+](CC)/CC6=CC=CC(S(=O)([O-])=O)=C6)C=C2)CC)=CC=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (14.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (11.71 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1709 mL	5.8547 mL	11.7093 mL
5 mM	0.2342 mL	1.1709 mL	2.3419 mL
10 mM	0.1171 mL	0.5855 mL	1.1709 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.56 mg/mL (0.66 mM); Clear solution

This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.56 mg/mL (0.66 mM); Clear solution

This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥95.0%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (257 KB)

Handling Instructions (2659 KB)

References

[1]. Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3. [Content Brief]

[2]. Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328. [Content Brief]

[3]. Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.1709 mL	5.8547 mL	11.7093 mL	29.2733 mL
	5 mM	0.2342 mL	1.1709 mL	2.3419 mL	5.8547 mL
	10 mM	0.1171 mL	0.5855 mL	1.1709 mL	2.9273 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.