2. Immunology/Inflammation Anti-infection Metabolic Enzyme/Protease
  3. STING Bacterial Endogenous Metabolite
  4. c-di-AMP disodium

c-di-AMP disodium  (Synonyms: Cyclic diadenylate disodium; Cyclic-di-AMP disodium)

Cat. No.: HY-12326A Purity: 99.71%
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c-di-AMP (Cyclic diadenylate) sodium is a STING agonist, which binds to the transmembrane protein STING thereby activating the TBK3-IRF3 signaling pathway, subsequently triggering the production of type I IFN and TNF. c-di-AMP sodium is also a bacterial second messenger, which regulates cell growth, survival, and virulence, primarily within Gram-positive bacteria, and also regulates host immune response. c-di-AMP sodium acts as a potent mucosal adjuvant stimulating both humoral and cellular responses.

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c-di-AMP disodium Chemical Structure

c-di-AMP disodium Chemical Structure

CAS No. : 2734909-87-4

Size Price Stock Quantity
500 μg USD 120 In-stock
1 mg USD 180 In-stock
5 mg USD 540 In-stock
10 mg USD 920 In-stock
25 mg USD 1800 In-stock
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Based on 2 publication(s) in Google Scholar

Other Forms of c-di-AMP disodium:

c-di-AMP disodium Related Antibodies

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Description

c-di-AMP (Cyclic diadenylate) sodium is a STING agonist, which binds to the transmembrane protein STING thereby activating the TBK3-IRF3 signaling pathway, subsequently triggering the production of type I IFN and TNF. c-di-AMP sodium is also a bacterial second messenger, which regulates cell growth, survival, and virulence, primarily within Gram-positive bacteria, and also regulates host immune response. c-di-AMP sodium acts as a potent mucosal adjuvant stimulating both humoral and cellular responses[1][2][3][4].

IC50 & Target

STING[3]
In Vitro

c-di-AMP (Cyclic diadenylate) sodium signaling is a central factor in many Gram-positive bacteria regulating cell wall synthesis, potassium ion channels, DNA repair, and biofilm formation. c-di-AMP sodium is also essential for cell growth, survival, and virulence of several well-known human pathogenic bacteria including S. aureus, L. monocytogenes, S. pyogenes, and Mycobacterium spp[1].
c-di-AMP sodium combines with model antigens, such as OVA or β-Gal, acts as a potent mucosal adjuvant stimulating both humoral and cellular responses[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

c-di-AMP disodium Related Antibodies
Molecular Weight

702.38

Formula

C20H22N10Na2O12P2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@@](OP(OC[C@](O[C@@H](N1C2=NC=NC(N)=C2N=C1)[C@@H]3O)([H])[C@@]3([H])O4)(O[Na])=O)([H])[C@](COP4(O[Na])=O)([H])O5)[C@@H]5N6C7=NC=NC(N)=C7N=C6
Structure Classification
Initial Source
Shipping

Shipping with dry ice.
Storage

-80°C, protect from light, stored under nitrogen
Solvent & Solubility
In Vitro: 

DMSO : 270 mg/mL (384.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 50 mg/mL (71.19 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4237 mL 7.1187 mL 14.2373 mL
5 mM 0.2847 mL 1.4237 mL 2.8475 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 6.75 mg/mL (9.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (67.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 6.75 mg/mL (9.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (67.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.4237 mL 7.1187 mL 14.2373 mL 35.5933 mL
5 mM 0.2847 mL 1.4237 mL 2.8475 mL 7.1187 mL
10 mM 0.1424 mL 0.7119 mL 1.4237 mL 3.5593 mL
15 mM 0.0949 mL 0.4746 mL 0.9492 mL 2.3729 mL
20 mM 0.0712 mL 0.3559 mL 0.7119 mL 1.7797 mL
25 mM 0.0569 mL 0.2847 mL 0.5695 mL 1.4237 mL
30 mM 0.0475 mL 0.2373 mL 0.4746 mL 1.1864 mL
40 mM 0.0356 mL 0.1780 mL 0.3559 mL 0.8898 mL
50 mM 0.0285 mL 0.1424 mL 0.2847 mL 0.7119 mL
60 mM 0.0237 mL 0.1186 mL 0.2373 mL 0.5932 mL
DMSO 80 mM 0.0178 mL 0.0890 mL 0.1780 mL 0.4449 mL
100 mM 0.0142 mL 0.0712 mL 0.1424 mL 0.3559 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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c-di-AMP disodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

c-di-AMP disodium2734909-87-4Cyclic diadenylate disodiumCyclic-di-AMP disodiumSTINGBacterialEndogenous MetaboliteStimulator of Interferon GenesTMEM173MITAERISMPYSendogenousGram-Positivebacteriacell growthsurvivalvirulenceInhibitorinhibitorinhibit

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