2. Metabolic Enzyme/Protease
  3. Carbonic Anhydrase
  4. CAIX Inhibitor S4

CAIX Inhibitor S4 is a potent and selective inhibitor of carbonic anhydrase IX/XII (CA IX/XII), with a Ki of 7 nM and 2 nM, respectively. CAIX Inhibitor S4 also inhibits CA II and CA I (Ki=546 and 5600 nM, respectively). CAIX Inhibitor S4 can inhibit the number of lung metastasis in orthotopic MDA-MB-231 mouse model without affecting primary tumor growth.

For research use only. We do not sell to patients.

CAIX Inhibitor S4 Chemical Structure

CAIX Inhibitor S4 Chemical Structure

CAS No. : 1330061-67-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 In-stock
Solution
10 mM * 1 mL in DMSO USD 77 In-stock
Solid
5 mg USD 70 In-stock
10 mg USD 120 In-stock
25 mg USD 250 In-stock
50 mg USD 450 In-stock
100 mg USD 750 In-stock
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Based on 1 publication(s) in Google Scholar

CAIX Inhibitor S4 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE CAIX Inhibitor S4

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CA CA I CA Ⅱ CA Ⅸ CA XII

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Description

CAIX Inhibitor S4 is a potent and selective inhibitor of carbonic anhydrase IX/XII (CA IX/XII), with a Ki of 7 nM and 2 nM, respectively. CAIX Inhibitor S4 also inhibits CA II and CA I (Ki=546 and 5600 nM, respectively). CAIX Inhibitor S4 can inhibit the number of lung metastasis in orthotopic MDA-MB-231 mouse model without affecting primary tumor growth[1].

IC50 & Target

CA Ⅸ

 

In Vitro

CAIX Inhibitor S4 (1-100 μM; 24 h) inhibits the proliferation of MDA-MB-231, HCT116 and HT29 cells in a dose-dependent manner[1].
CAIX Inhibitor S4 (3.3-33 μM; 24 h) inhibits the eGFP-MDA-MB-231 cell migration in anoxia in a concentration- dependent manner[1].
CAIX Inhibitor S4 (33 μM; 15-60 min) delays the cell spreading of MDA-MB-231 cells in anoxia but essentially not in normoxia[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CAIX Inhibitor S4 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MDA-MB-231, HCT116 and HT29 cells
Concentration: 1, 10, 100 μM
Incubation Time: 24 hours
Result: Inhibited the cell proliferation of MDA-MB-231, HCT116 and HT29 cells, with IC50s of 481 μM, >1000 μM, and 20 μM, respectively.
In Vivo

CAIX Inhibitor S4 (10 mg/kg; i.p. for 14 days) inhibits metastatic tumor burden in MDA-MB-231 model while having no effect on primary tumor growth or mouse condition[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female nu/nu CBA mice (10-12 weeks) were injected with eGFP-MDA-MB-231 cells[1]
Dosage: 10 mg/kg
Administration: I.p. daily on a “5 days on, 2 days off” dosing regimen for 14 days
Result: Significantly reduced the metastatic tumor burden in lungs of mice bearing orthotopic eGFP-MDA-MB-231 tumors.
The average body weights between vehicle and S4 treated mice were similar throughout the experiments.
Molecular Weight

335.38

Formula

C15H17N3O4S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC(C)=CC(C)=C1)NC2=CC=C(C=C2)OS(=O)(N)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (745.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9817 mL 14.9085 mL 29.8169 mL
5 mM 0.5963 mL 2.9817 mL 5.9634 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9817 mL 14.9085 mL 29.8169 mL 74.5423 mL
5 mM 0.5963 mL 2.9817 mL 5.9634 mL 14.9085 mL
10 mM 0.2982 mL 1.4908 mL 2.9817 mL 7.4542 mL
15 mM 0.1988 mL 0.9939 mL 1.9878 mL 4.9695 mL
20 mM 0.1491 mL 0.7454 mL 1.4908 mL 3.7271 mL
25 mM 0.1193 mL 0.5963 mL 1.1927 mL 2.9817 mL
30 mM 0.0994 mL 0.4969 mL 0.9939 mL 2.4847 mL
40 mM 0.0745 mL 0.3727 mL 0.7454 mL 1.8636 mL
50 mM 0.0596 mL 0.2982 mL 0.5963 mL 1.4908 mL
60 mM 0.0497 mL 0.2485 mL 0.4969 mL 1.2424 mL
80 mM 0.0373 mL 0.1864 mL 0.3727 mL 0.9318 mL
100 mM 0.0298 mL 0.1491 mL 0.2982 mL 0.7454 mL
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CAIX Inhibitor S4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CAIX Inhibitor S41330061-67-0CAIX Inhibitor S 4CAIX Inhibitor S-4Carbonic AnhydraseCarbonate dehydrataseIXXIICAIIIlungmetastasisorthotopicMDA-MB-231tumorInhibitorinhibitorinhibit

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