CASP8-IN-1

Cat. No.: HY-153161 Purity: 98.97%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

CASP8-IN-1 (Compound 63-R) is a selective inhibitor for caspase 8 (CASP8), with an IC₅₀ of 0.7 μM. CASP8-IN-1 inhibits FasL-induced apoptosis in cell Jurkat.

For research use only. We do not sell to patients.

CASP8-IN-1 Chemical Structure

CAS No. : 1956368-39-0

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

CASP8-IN-1 (Compound 63-R) is a selective inhibitor for caspase 8 (CASP8), with an IC₅₀ of 0.7 μM. CASP8-IN-1 inhibits FasL-induced apoptosis in cell Jurkat^[1].

IC₅₀ & Target

Caspase 8

0.7 μM (IC₅₀)

Molecular Weight

441.95

Formula

C₂₄H₂₈ClN₃O₃

CAS No.

1956368-39-0

Appearance

Solid

Color

White to off-white

SMILES

ClCC(N(C1=CC=CC=C1)[C@H]2CN(CCC2)C(C3=CC=C(N4CCOCC4)C=C3)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (226.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2627 mL	11.3135 mL	22.6270 mL
5 mM	0.4525 mL	2.2627 mL	4.5254 mL
10 mM	0.2263 mL	1.1313 mL	2.2627 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.97%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (250 KB) HNMR (263 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Backus KM, et al., Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2627 mL	11.3135 mL	22.6270 mL	56.5675 mL
	5 mM	0.4525 mL	2.2627 mL	4.5254 mL	11.3135 mL
	10 mM	0.2263 mL	1.1313 mL	2.2627 mL	5.6567 mL
	15 mM	0.1508 mL	0.7542 mL	1.5085 mL	3.7712 mL
	20 mM	0.1131 mL	0.5657 mL	1.1313 mL	2.8284 mL
	25 mM	0.0905 mL	0.4525 mL	0.9051 mL	2.2627 mL
	30 mM	0.0754 mL	0.3771 mL	0.7542 mL	1.8856 mL
	40 mM	0.0566 mL	0.2828 mL	0.5657 mL	1.4142 mL
	50 mM	0.0453 mL	0.2263 mL	0.4525 mL	1.1313 mL
	60 mM	0.0377 mL	0.1886 mL	0.3771 mL	0.9428 mL
	80 mM	0.0283 mL	0.1414 mL	0.2828 mL	0.7071 mL
	100 mM	0.0226 mL	0.1131 mL	0.2263 mL	0.5657 mL

CASP8-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CASP8-IN-11956368-39-0CaspaseApoptosisJurkatselectiveInhibitorinhibitorinhibit

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