CCR4 antagonist 4

Cat. No.: HY-157453 Purity: 99.72%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

CCR4 antagonist 4 (compound 22) is a selective and potent antagonist of the CC chemokine receptor-4 (CCR4), with an IC₅₀ of 0.02 μM. CCR4 antagonist 4 also blocks MDC-mediated chemotaxis (IC₅₀: 0.007 μM) and Ca²⁺ mobilization (IC₅₀: 0.003 μM). CCR4 antagonist 4 can be used for allergic inflammation research.

For research use only. We do not sell to patients.

CCR4 antagonist 4 Chemical Structure

CAS No. : 668980-17-4

Size	Price	Stock	Quantity
5 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 725	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1450	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2320	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3710	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 CCR9

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

CCR4

0.02 μM (IC₅₀)

Molecular Weight

500.42

Formula

C₂₄H₂₇Cl₂N₇O

CAS No.

668980-17-4

Appearance

Solid

Color

White to off-white

SMILES

ClC(C=C(C=C1)Cl)=C1CNC2=NC(N3CCN(CC3)C([C@H]4CCCCN4)=O)=NC5=C2C=CC=N5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (199.83 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9983 mL	9.9916 mL	19.9832 mL
5 mM	0.3997 mL	1.9983 mL	3.9966 mL
10 mM	0.1998 mL	0.9992 mL	1.9983 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (252 KB) HNMR (145 KB) RP-HPLC (174 KB) LCMS (379 KB)

Handling Instructions (2659 KB)

References

[1]. Purandare AV, et al. Core exploration in optimization of chemokine receptor CCR4 antagonists. Bioorg Med Chem Lett. 2007 Feb 1;17(3):679-82. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9983 mL	9.9916 mL	19.9832 mL	49.9580 mL
	5 mM	0.3997 mL	1.9983 mL	3.9966 mL	9.9916 mL
	10 mM	0.1998 mL	0.9992 mL	1.9983 mL	4.9958 mL
	15 mM	0.1332 mL	0.6661 mL	1.3322 mL	3.3305 mL
	20 mM	0.0999 mL	0.4996 mL	0.9992 mL	2.4979 mL
	25 mM	0.0799 mL	0.3997 mL	0.7993 mL	1.9983 mL
	30 mM	0.0666 mL	0.3331 mL	0.6661 mL	1.6653 mL
	40 mM	0.0500 mL	0.2498 mL	0.4996 mL	1.2490 mL
	50 mM	0.0400 mL	0.1998 mL	0.3997 mL	0.9992 mL
	60 mM	0.0333 mL	0.1665 mL	0.3331 mL	0.8326 mL
	80 mM	0.0250 mL	0.1249 mL	0.2498 mL	0.6245 mL
	100 mM	0.0200 mL	0.0999 mL	0.1998 mL	0.4996 mL