CGP55845 hydrochloride

Name: CGP55845 hydrochloride
Brand: MedChemExpress
SKU: HY-103516
Rating: 4.5 (1 reviews)

Cat. No.: HY-103516 Purity: 98.27%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

CGP55845 hydrochloride is a potent and selective GABAB receptor antagonist with an IC₅₀ of 6 nM. CGP55845 hydrochloride can be used for neurological research.

For research use only. We do not sell to patients.

CGP55845 hydrochloride Chemical Structure

CAS No. : 149184-22-5

Size	Price	Stock	Quantity
1 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 460	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of CGP55845 hydrochloride:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

CGP55845 hydrochloride is a potent and selective GABAB receptor antagonist with an IC₅₀ of 6 nM. CGP55845 hydrochloride can be used for neurological research^[1]^[2].

In Vitro

In the mouse visual cortex, CGP 55845 hydrochloride (1 μM) increases the frequency of spontaneous Ca²⁺ transients and spontaneous and miniature IPSCs (mIPSCs) but does not affect mIPSC amplitudes or kinetics. CGP55845 hydrochloride significantly increases evoked IPSC (eIPSC) amplitudes and decreases the paired-pulse ratio (PPR)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

CGP55845 hydrochloride (i.p.; once; 0.5 mg/kg) treatment increases the number of PSD95 positive puncta as well as density and maturation of dendritic spines in the perirhinal cortex (PRC), and restores novel object recognition (NOR) memory in Cdkl5 KO mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Cdkl5 +/Y and Cdkl5 -/Y male mice (2-month-old)^[1]
Dosage:	0.5 mg/kg
Administration:	i.p.; once
Result:	Altered NOR recognition memory in Cdkl5 -/Y mice

Molecular Weight

438.71

Formula

C₁₈H₂₃Cl₃NO₃P

CAS No.

149184-22-5

Appearance

Solid

Color

White to off-white

SMILES

O=P(CC1=CC=CC=C1)(C[C@@H](O)CN[C@H](C2=CC=C(Cl)C(Cl)=C2)C)O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (227.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2794 mL	11.3970 mL	22.7941 mL
5 mM	0.4559 mL	2.2794 mL	4.5588 mL
10 mM	0.2279 mL	1.1397 mL	2.2794 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (247 KB)

Handling Instructions (2659 KB)

References

[1]. Laura Gennaccaro, et al. A GABA B receptor antagonist rescues functional and structural impairments in the perirhinal cortex of a mouse model of CDKL5 deficiency disorder. Neurobiol Dis. 2021 Jun;153:105304. [Content Brief]

[2]. Claire-Marie Vacher, et al. GABA(B) receptors as potential therapeutic targets. Curr Drug Targets CNS Neurol Disord. 2003 Aug;2(4):248-59. [Content Brief]

[3]. Knut Kirmse, et al. Ambient GABA constrains the strength of GABAergic synapses at Cajal-Retzius cells in the developing visual cortex. J Neurosci. 2006 Apr 19;26(16):4216-27. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2794 mL	11.3971 mL	22.7941 mL	56.9853 mL
	5 mM	0.4559 mL	2.2794 mL	4.5588 mL	11.3971 mL
	10 mM	0.2279 mL	1.1397 mL	2.2794 mL	5.6985 mL
	15 mM	0.1520 mL	0.7598 mL	1.5196 mL	3.7990 mL
	20 mM	0.1140 mL	0.5699 mL	1.1397 mL	2.8493 mL
	25 mM	0.0912 mL	0.4559 mL	0.9118 mL	2.2794 mL
	30 mM	0.0760 mL	0.3799 mL	0.7598 mL	1.8995 mL
	40 mM	0.0570 mL	0.2849 mL	0.5699 mL	1.4246 mL
	50 mM	0.0456 mL	0.2279 mL	0.4559 mL	1.1397 mL
	60 mM	0.0380 mL	0.1900 mL	0.3799 mL	0.9498 mL
	80 mM	0.0285 mL	0.1425 mL	0.2849 mL	0.7123 mL
	100 mM	0.0228 mL	0.1140 mL	0.2279 mL	0.5699 mL