1. Anti-infection
  2. Bacterial
  3. Cholera autoinducer 1

Cholera autoinducer 1  (Synonyms: CAI-1)

Cat. No.: HY-145376 Purity: ≥99.0%
Data Sheet Handling Instructions Technical Support

Cholera autoinducer 1 is Vibrio cholerae autoinducer (S)-3-hydroxytridecan-4-one. Cholera autoinducer 1 influences chemotaxis in C. elegans.

For research use only. We do not sell to patients.

Cholera autoinducer 1 Chemical Structure

Cholera autoinducer 1 Chemical Structure

CAS No. : 1004296-82-5

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1 mg USD 160 In-stock
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Description

Cholera autoinducer 1 is Vibrio cholerae autoinducer (S)-3-hydroxytridecan-4-one. Cholera autoinducer 1 influences chemotaxis in C. elegans[1].

Molecular Weight

214.34

Formula

C13H26O2

CAS No.
Appearance

Oil

Color

Colorless to light yellow

SMILES

CCCCCCCCCC([C@@H](O)CC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (233.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.6655 mL 23.3274 mL 46.6548 mL
5 mM 0.9331 mL 4.6655 mL 9.3310 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (5.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (5.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: ≥99.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.6655 mL 23.3274 mL 46.6548 mL 116.6371 mL
5 mM 0.9331 mL 4.6655 mL 9.3310 mL 23.3274 mL
10 mM 0.4665 mL 2.3327 mL 4.6655 mL 11.6637 mL
15 mM 0.3110 mL 1.5552 mL 3.1103 mL 7.7758 mL
20 mM 0.2333 mL 1.1664 mL 2.3327 mL 5.8319 mL
25 mM 0.1866 mL 0.9331 mL 1.8662 mL 4.6655 mL
30 mM 0.1555 mL 0.7776 mL 1.5552 mL 3.8879 mL
40 mM 0.1166 mL 0.5832 mL 1.1664 mL 2.9159 mL
50 mM 0.0933 mL 0.4665 mL 0.9331 mL 2.3327 mL
60 mM 0.0778 mL 0.3888 mL 0.7776 mL 1.9440 mL
80 mM 0.0583 mL 0.2916 mL 0.5832 mL 1.4580 mL
100 mM 0.0467 mL 0.2333 mL 0.4665 mL 1.1664 mL
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Product Name:
Cholera autoinducer 1
Cat. No.:
HY-145376
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