Cholera autoinducer 1 (Synonyms: CAI-1)

Cat. No.: HY-145376 Purity: ≥99.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Cholera autoinducer 1 is Vibrio cholerae autoinducer (S)-3-hydroxytridecan-4-one. Cholera autoinducer 1 influences chemotaxis in C. elegans.

For research use only. We do not sell to patients.

Cholera autoinducer 1 Chemical Structure

CAS No. : 1004296-82-5

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	Get quote
10 mg	Get quote

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Description

Cholera autoinducer 1 is Vibrio cholerae autoinducer (S)-3-hydroxytridecan-4-one. Cholera autoinducer 1 influences chemotaxis in C. elegans^[1].

Molecular Weight

214.34

Formula

C₁₃H₂₆O₂

CAS No.

1004296-82-5

Appearance

Oil

Color

Colorless to light yellow

SMILES

CCCCCCCCCC([C@@H](O)CC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (233.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6655 mL	23.3274 mL	46.6548 mL
5 mM	0.9331 mL	4.6655 mL	9.3310 mL
10 mM	0.4665 mL	2.3327 mL	4.6655 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (5.83 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (5.83 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (5.83 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (266 KB) SDS (252 KB)

COA (240 KB)

Handling Instructions (2659 KB)

References

[1]. Werner KM, et al. Caenorhabditis elegans recognizes a bacterial quorum-sensing signal molecule through the AWCON neuron. J Biol Chem. 2014;289(38):26566-26573. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.6655 mL	23.3274 mL	46.6548 mL	116.6371 mL
	5 mM	0.9331 mL	4.6655 mL	9.3310 mL	23.3274 mL
	10 mM	0.4665 mL	2.3327 mL	4.6655 mL	11.6637 mL
	15 mM	0.3110 mL	1.5552 mL	3.1103 mL	7.7758 mL
	20 mM	0.2333 mL	1.1664 mL	2.3327 mL	5.8319 mL
	25 mM	0.1866 mL	0.9331 mL	1.8662 mL	4.6655 mL
	30 mM	0.1555 mL	0.7776 mL	1.5552 mL	3.8879 mL
	40 mM	0.1166 mL	0.5832 mL	1.1664 mL	2.9159 mL
	50 mM	0.0933 mL	0.4665 mL	0.9331 mL	2.3327 mL
	60 mM	0.0778 mL	0.3888 mL	0.7776 mL	1.9440 mL
	80 mM	0.0583 mL	0.2916 mL	0.5832 mL	1.4580 mL
	100 mM	0.0467 mL	0.2333 mL	0.4665 mL	1.1664 mL

Cholera autoinducer 1 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cholera autoinducer 11004296-82-5CAI-1Cholera autoinducer1Cholera autoinducer-1CAI1CAI 1BacterialVibrio choleraautoinducerchemotaxisC. elegansInhibitorinhibitorinhibit

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Cholera autoinducer 1 (Synonyms: CAI-1)

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