2. PROTAC Apoptosis
  3. PROTACs IAP
  4. CST626

CST626 (Compound 9) is a pan-IAP degrader PROTAC. CST626 degrades XIAP, cIAP1 and cIAP2 with DC50s of 0.7, 2.4, and 6.2 nM in MM.1S cells, respectively.

For research use only. We do not sell to patients.

CST626 Chemical Structure

CST626 Chemical Structure

CAS No. : 3033993-13-1

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1 mg USD 504 In-stock
5 mg USD 1260 In-stock
10 mg USD 2135 In-stock
25 mg USD 4475 In-stock
50 mg USD 7600 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

CST626 Related Antibodies

Top Publications Citing Use of Products

View All PROTACs Isoform Specific Products:

View All Isoforms
von Hippel-Lindau (VHL) Cereblon MDM2 cIAP1

View All IAP Isoform Specific Products:

View All Isoforms
cIAP cIAP-1 cIAP-2 XIAP

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CST626 (Compound 9) is a pan-IAP degrader PROTAC. CST626 degrades XIAP, cIAP1 and cIAP2 with DC50s of 0.7, 2.4, and 6.2 nM in MM.1S cells, respectively[1].

In Vitro

CST626 (Compound 9) induces cIAP1, cIAP2 and XIAP degradation in a dose-dependent manner with DC50 values of 2.4 nM, 6.2 nM, and 0.7 nM, respectively[1].
CST626 (0-10 μM; 96 h) shows potent inhibition of cancer cell viability[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CST626 Related Antibodies

Western Blot Analysis[1]

Cell Line: MM.1S cells
Concentration: 0.0001, 0.001, 0.01, 0.1 and 1 μM
Incubation Time: 16 h
Result: Revealed DC50 values of 2.4 nM (cIAP1), 6.2 nM (cIAP2), and 0.7 nM (XIAP).

Cell Viability Assay[1]

Cell Line: SUDHL6, MOLM13, NCI-H929, K562, DB, JJN3, HEL, SUDHL4 and RPMI-8826 cells
Concentration: 0.01, 0.04, 0.1, 0.4, 1, 4 and 10 μM
Incubation Time: 96 h
Result: Inhibited cell viability with IC50s of 0.0016, 0.0021, 0.0085, 0.42, 0.46, 1.14, 1.17, 1.69 and 2.54 μM against SUDHL6, MOLM13, NCI-H929, K562, DB, JJN3, HEL, SUDHL4 and RPMI-8826 cells, respectively.
Molecular Weight

1103.42

Formula

C61H82N8O9S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H]1N(C([C@H](C2CCCCC2)NC([C@@H](NC)C)=O)=O)C[C@@H](OC3=CC=CC(OCCCCC(N[C@@H](C(C)(C)C)C(N4[C@H](C(N[C@H](C5=CC=C(C6=C(C)N=CS6)C=C5)C)=O)C[C@@H](O)C4)=O)=O)=C3)C1)N[C@@H]7CCCC8=C7C=CC=C8
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (90.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9063 mL 4.5314 mL 9.0627 mL
5 mM 0.1813 mL 0.9063 mL 1.8125 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (2.27 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (2.27 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9063 mL 4.5314 mL 9.0627 mL 22.6568 mL
5 mM 0.1813 mL 0.9063 mL 1.8125 mL 4.5314 mL
10 mM 0.0906 mL 0.4531 mL 0.9063 mL 2.2657 mL
15 mM 0.0604 mL 0.3021 mL 0.6042 mL 1.5105 mL
20 mM 0.0453 mL 0.2266 mL 0.4531 mL 1.1328 mL
25 mM 0.0363 mL 0.1813 mL 0.3625 mL 0.9063 mL
30 mM 0.0302 mL 0.1510 mL 0.3021 mL 0.7552 mL
40 mM 0.0227 mL 0.1133 mL 0.2266 mL 0.5664 mL
50 mM 0.0181 mL 0.0906 mL 0.1813 mL 0.4531 mL
60 mM 0.0151 mL 0.0755 mL 0.1510 mL 0.3776 mL
80 mM 0.0113 mL 0.0566 mL 0.1133 mL 0.2832 mL
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CST626 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CST6263033993-13-1CST 626CST-626PROTACsIAPMM.1SSUDHL6MOLM13NCI-H929K562DBJJN3HELSUDHL4RPMI-8826Inhibitorinhibitorinhibit

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