Cy 5 hydrazide  (Synonyms: Cyanine5 hydrazide)

Cy 5 hydrazide (Cyanine5 hydrazide) is a fluorescent dye for aldehydes and ketones. Cy 5 hydrazide can be used for labeling proteins^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability assay^[1]:
1. Add Cy 5 hydrazide to each sample at 25℃ for 1 h to label sample.
2. Remove salts and unbound fluorescent dye by the trichloroacetic acid (TCA esodium deoxycholate (DOC) or acetone method.
3. Measure fluorescence intensity (Ex=640 nm, Em=660 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

569.61

C₃₂H₄₂Cl₂N₄O

1427705-31-4

Solid

Dark purple to black

670

645

O=C(NN)CCCCC[N+](C1=CC=CC=C1C(C)2C)=C2/C=C/C=C/C=C(C3(C)C)\N(C4=CC=CC=C34)C.[Cl-].Cl

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Yoshimura T, et al. A novel procedure for the assessment of the antioxidant capacity of food components. Anal Biochem. 2016 Aug 15;507:7-12.  [Content Brief]