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  4. Cy 5 hydrazide

Cy 5 hydrazide  (Synonyms: Cyanine5 hydrazide)

Cat. No.: HY-137100 Purity: 95.00%
SDS
COA
Handling Instructions Technical Support

Cy 5 hydrazide (Cyanine5 hydrazide) is a fluorescent dye for aldehydes and ketones. Cy 5 hydrazide can be used for labeling proteins.

For research use only. We do not sell to patients.

Cy 5 hydrazide Chemical Structure

Cy 5 hydrazide Chemical Structure

CAS No. : 1427705-31-4

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1 mg In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Cy 5 hydrazide Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cy 5 hydrazide (Cyanine5 hydrazide) is a fluorescent dye for aldehydes and ketones. Cy 5 hydrazide can be used for labeling proteins[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability assay[1]:
1. Add Cy 5 hydrazide to each sample at 25℃ for 1 h to label sample.
2. Remove salts and unbound fluorescent dye by the trichloroacetic acid (TCA esodium deoxycholate (DOC) or acetone method.
3. Measure fluorescence intensity (Ex=640 nm, Em=660 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cy 5 hydrazide Related Antibodies
Molecular Weight

569.61

Formula

C32H42Cl2N4O
CAS No.
Appearance

Solid

Color

Dark purple to black

Emission (Em)

670
Excitation (Ex)

645
SMILES

O=C(NN)CCCCC[N+](C1=CC=CC=C1C(C)2C)=C2/C=C/C=C/C=C(C3(C)C)\N(C4=CC=CC=C34)C.[Cl-].Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (87.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7556 mL 8.7779 mL 17.5559 mL
5 mM 0.3511 mL 1.7556 mL 3.5112 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 95.00%

SDS (252 KB)

COA (259 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7556 mL 8.7779 mL 17.5559 mL 43.8897 mL
5 mM 0.3511 mL 1.7556 mL 3.5112 mL 8.7779 mL
10 mM 0.1756 mL 0.8778 mL 1.7556 mL 4.3890 mL
15 mM 0.1170 mL 0.5852 mL 1.1704 mL 2.9260 mL
20 mM 0.0878 mL 0.4389 mL 0.8778 mL 2.1945 mL
25 mM 0.0702 mL 0.3511 mL 0.7022 mL 1.7556 mL
30 mM 0.0585 mL 0.2926 mL 0.5852 mL 1.4630 mL
40 mM 0.0439 mL 0.2194 mL 0.4389 mL 1.0972 mL
50 mM 0.0351 mL 0.1756 mL 0.3511 mL 0.8778 mL
60 mM 0.0293 mL 0.1463 mL 0.2926 mL 0.7315 mL
80 mM 0.0219 mL 0.1097 mL 0.2194 mL 0.5486 mL
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Cy 5 hydrazide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cy 5 hydrazide1427705-31-4Cyanine5 hydrazideFluorescent DyefluorescentdyealdehydeketoneInhibitorinhibitorinhibit

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