Eriocalyxin B 

Eriocalyxin B is a diterpenoid compound that can be isolated from Chinese herb Isodon eriocalyx. Eriocalyxin B exhibits multiple activities, such as anti-cancer, anti-inflammatory, and inhibition of adipogenesis. Eriocalyxin B is capable of inducing apoptosis and autophagy in tumor cells. Eriocalyxin B can be used in the research of cancers, autoimmune diseases, and other conditions^{[1][2][3][4][5]}.

Eriocalyxin B (0-3.5 μM; 7 days) inhibits adipogenesis (IC₅₀: 2.745 μM) and suppresses the expression of key regulators of adipocyte differentiation, including C/EBPα, C/EBPβ, PPARγ, and FABP4 in 3T3-L1 preadipocytes^[1].
Eriocalyxin B (1.25-3.5 μM; 16-48 h) induces G2/M phase arrest and inhibits the expression levels of cell cycle regulators in differentiated 3T3-L1 cells^[1].
Eriocalyxin B (48 h) is cytotoxic to tumor cells such as A-549, MCF-7, SMMC-7721, SW-480, and HL-60, with IC₅₀ values ranging from 0.3-3.1 μM^[2].
Eriocalyxin B (0.35-2.25 μM; 24-72 h) inhibits cell viability, increases intracellular ROS production, and induces apoptosis and autophagy in MCF-7 and MDA-MB-231. The mechanism involves the inhibition of the Akt/mTOR/p70S6K signaling pathway^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Eriocalyxin B (10 mg/kg; intraperitoneal injection; 27 days) exhibits anti-tumor activity in a mouse model of breast cancer^[3].
Eriocalyxin B (10 mg/kg; intraperitoneal injection; 18-28 days) has an ameliorating effect in a mouse model of experimental autoimmune encephalomyelitis (EAE)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

344.40

C₂₀H₂₄O₅

84745-95-9

Solid

White to off-white

O[C@]1(OC2)[C@](C[C@]3([H])C4=C)(C4=O)[C@](CC3)([H])[C@]2(C(C=C5)=O)[C@](C5(C)C)([H])[C@@H]1O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Rong-Fang Mu, et al. Eriocalyxin B Inhibits Adipogenesis in 3T3-L1 Adipocytes by Cell Cycle Arrest. Nat Prod Bioprospect. 2020 Jun;10(3):131-140.  [Content Brief]

  [2]. Wang WG, et al. Laxiflorolides A and B, epimeric bishomoditerpene lactones from Isodon eriocalyx. J Nat Prod. 2012 Jun 22;75(6):1102-7.  [Content Brief]

  [3]. Zhou X, et al. Eriocalyxin B, a novel autophagy inducer, exerts anti-tumor activity through the suppression of Akt/mTOR/p70S6K signaling pathway in breast cancer. Biochem Pharmacol. 2017 Oct 15;142:58-70.  [Content Brief]

  [4]. Lu Y, et al. Eriocalyxin B ameliorates experimental autoimmune encephalomyelitis by suppressing Th1 and Th17 cells. Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):2258-63.  [Content Brief]

  [5]. Leung CH, et al. Eriocalyxin B inhibits nuclear factor-kappaB activation by interfering with the binding of both p65 and p50 to the response element in a noncompetitive manner. Mol Pharmacol. 2006 Dec;70(6):1946-55.  [Content Brief]