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  4. CY5.5-COOH

CY5.5-COOH  (Synonyms: Cyanine 5.5 carboxylic acid)

Cat. No.: HY-D1040 Purity: 98.99%
COA Handling Instructions

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CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye, is commonly used in bioimaging. CY5.5-COOH shows narrow absorption spectrum, and high sensitivity and stability.

For research use only. We do not sell to patients.

CY5.5-COOH Chemical Structure

CY5.5-COOH Chemical Structure

CAS No. : 1449612-07-0

Size Price Stock Quantity
5 mg USD 120 In-stock
10 mg USD 200 In-stock
25 mg USD 400 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of CY5.5-COOH:

CY5.5-COOH Related Antibodies

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Description

CY5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye, is commonly used in bioimaging. CY5.5-COOH shows narrow absorption spectrum, and high sensitivity and stability[1].

Molecular Weight

582.77

Formula

C40H42N2O2
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

C[N+]1=C(/C=C/C=C/C=C2N(CCCCCC([O-])=O)C3=C(C4=CC=CC=C4C=C3)C/2(C)C)C(C)(C)C5=C1C=CC6=CC=CC=C56
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (85.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7159 mL 8.5797 mL 17.1594 mL
5 mM 0.3432 mL 1.7159 mL 3.4319 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.14 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.99%

COA (254 KB) HNMR (299 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7159 mL 8.5797 mL 17.1594 mL 42.8986 mL
5 mM 0.3432 mL 1.7159 mL 3.4319 mL 8.5797 mL
10 mM 0.1716 mL 0.8580 mL 1.7159 mL 4.2899 mL
15 mM 0.1144 mL 0.5720 mL 1.1440 mL 2.8599 mL
20 mM 0.0858 mL 0.4290 mL 0.8580 mL 2.1449 mL
25 mM 0.0686 mL 0.3432 mL 0.6864 mL 1.7159 mL
30 mM 0.0572 mL 0.2860 mL 0.5720 mL 1.4300 mL
40 mM 0.0429 mL 0.2145 mL 0.4290 mL 1.0725 mL
50 mM 0.0343 mL 0.1716 mL 0.3432 mL 0.8580 mL
60 mM 0.0286 mL 0.1430 mL 0.2860 mL 0.7150 mL
80 mM 0.0214 mL 0.1072 mL 0.2145 mL 0.5362 mL
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CY5.5-COOH Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CY5.5-COOH1449612-07-0Cyanine 5.5 carboxylic acidFluorescent Dyefluorescent dyehigh sensitivityInhibitorinhibitorinhibit

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