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  4. Cyanine5.5 tetrazine

Cyanine5.5 tetrazine is a far-infrared luminescent dye. Cyanine5.5 tetrazine is a Cyanine5.5 (HY-D0925A) derivative contains a tetrazine moiety. Cyanine5.5 tetrazine can be used for in vivo imaging and low background applications. Cyanine5.5 tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

For research use only. We do not sell to patients.

Cyanine5.5 tetrazine Chemical Structure

Cyanine5.5 tetrazine Chemical Structure

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Based on 1 publication(s) in Google Scholar

Cyanine5.5 tetrazine Related Antibodies

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Description

Cyanine5.5 tetrazine is a far-infrared luminescent dye. Cyanine5.5 tetrazine is a Cyanine5.5 (HY-D0925A) derivative contains a tetrazine moiety. Cyanine5.5 tetrazine can be used for in vivo imaging and low background applications. Cyanine5.5 tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

Molecular Weight

911.96

Formula

C50H52F6N7OP
Appearance

Solid

Color

Purple to purplish red

Emission (Em)

710
Excitation (Ex)

680
SMILES

CC1=NN=C(N=N1)C2=CC=C(C=C2)CNC(CCCCC[N+]3=C(C(C)(C4=C3C=CC5=C4C=CC=C5)C)/C=C/C=C/C=C6N(C7=C(C/6(C)C)C8=C(C=C7)C=CC=C8)C)=O.[F-][P+5]([F-])([F-])([F-])([F-])[F-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (109.65 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0965 mL 5.4827 mL 10.9654 mL
5 mM 0.2193 mL 1.0965 mL 2.1931 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (2.74 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.74 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0965 mL 5.4827 mL 10.9654 mL 27.4135 mL
5 mM 0.2193 mL 1.0965 mL 2.1931 mL 5.4827 mL
10 mM 0.1097 mL 0.5483 mL 1.0965 mL 2.7413 mL
15 mM 0.0731 mL 0.3655 mL 0.7310 mL 1.8276 mL
20 mM 0.0548 mL 0.2741 mL 0.5483 mL 1.3707 mL
25 mM 0.0439 mL 0.2193 mL 0.4386 mL 1.0965 mL
30 mM 0.0366 mL 0.1828 mL 0.3655 mL 0.9138 mL
40 mM 0.0274 mL 0.1371 mL 0.2741 mL 0.6853 mL
50 mM 0.0219 mL 0.1097 mL 0.2193 mL 0.5483 mL
60 mM 0.0183 mL 0.0914 mL 0.1828 mL 0.4569 mL
80 mM 0.0137 mL 0.0685 mL 0.1371 mL 0.3427 mL
100 mM 0.0110 mL 0.0548 mL 0.1097 mL 0.2741 mL
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Cyanine5.5 tetrazine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyanine5.5 tetrazineFluorescent Dyevivo imagingtetrazinelow backgroundInhibitorinhibitorinhibit

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