Cyanine5 NHS ester chloride

Cat. No.: HY-135414: FAQs
Data Sheet Handling Instructions

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Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.

For research use only. We do not sell to patients.

Cyanine5 NHS ester chloride Chemical Structure

CAS No. : 1032678-42-4

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Other Forms of Cyanine5 NHS ester chloride:

Cyanine 5 NHS ester tetrafluoroborate Get quote
Cyanine5 NHS ester bromide Get quote
Cyanine5 NHS ester iodide Get quote

1 Publications Citing Use of MCE Cyanine5 NHS ester chloride

•Commun Biol. 2022 Oct 3;5(1):1052. [Abstract]

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Biological Activity
Purity & Documentation
References
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Description

Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides^[1].

In Vitro

Cyanine-5 NHS ester amine-reactive red emitting fluorescent dye is used to react with the free amino group on the cysteine amino acid present in terminal peptides used for end-capping Poly(β-amino esters) (pBAEs) backbones^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

616.19

Formula

C₃₆H₄₂ClN₃O₄

CAS No.

1032678-42-4

SMILES

CC1(C)C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=C1C=CC=C5.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (405.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6229 mL	8.1144 mL	16.2288 mL
5 mM	0.3246 mL	1.6229 mL	3.2458 mL
10 mM	0.1623 mL	0.8114 mL	1.6229 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Brugada-Vilà P, et al. Oligopeptide-modified poly(beta-amino ester)s-coated AdNuPARmE1A: Boosting the efficacy of intravenously administered therapeutic adenoviruses. Theranostics. 2020;10(6):2744-2758. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6229 mL	8.1144 mL	16.2288 mL	40.5719 mL
	5 mM	0.3246 mL	1.6229 mL	3.2458 mL	8.1144 mL
	10 mM	0.1623 mL	0.8114 mL	1.6229 mL	4.0572 mL
	15 mM	0.1082 mL	0.5410 mL	1.0819 mL	2.7048 mL
	20 mM	0.0811 mL	0.4057 mL	0.8114 mL	2.0286 mL
	25 mM	0.0649 mL	0.3246 mL	0.6492 mL	1.6229 mL
	30 mM	0.0541 mL	0.2705 mL	0.5410 mL	1.3524 mL
	40 mM	0.0406 mL	0.2029 mL	0.4057 mL	1.0143 mL
	50 mM	0.0325 mL	0.1623 mL	0.3246 mL	0.8114 mL
	60 mM	0.0270 mL	0.1352 mL	0.2705 mL	0.6762 mL
	80 mM	0.0203 mL	0.1014 mL	0.2029 mL	0.5071 mL
	100 mM	0.0162 mL	0.0811 mL	0.1623 mL	0.4057 mL