CypK (Synonyms: N-Cyclopropene-L-Lysine)

Name: CypK
Brand: MedChemExpress
SKU: HY-134669
Rating: 4.5 (1 reviews)

Cat. No.: HY-134669 Purity: 98.30%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

CypK (N-Cyclopropene-L-Lysine), a cyclopropene derivative of lysine, is efficiently incorporated into antibodies through genetic-code expansion. CypK is a minimal bioorthogonal handle for the creation of stable therapeutic protein conjugates.

For research use only. We do not sell to patients.

CypK Chemical Structure

CAS No. : 1610703-09-7

Size	Price	Stock	Quantity
5 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1600	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2600	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4200	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
CypK assay^[1] (express the antibody):
1. Thaw a vial of HEK suspension cells in a 250 mL flask containing 50 mL of expression medium supplemented with 100 units/mL penicillin, 100 µg/mL streptomycin, and 250 ng/mL amphotericin B. Keep the cells at 37 ℃ with 8% CO₂ in humidified incubators equipped with a shaker at 125 rpm. Split cells to 0.3-0.5 x 10⁶ cells/mL (every 2-3 days) at least 2 times before transfecting.
2. When a density of 2.5 x 10⁶ cells/mL is reached (2-3 days after splitting), prepare a fresh solution of 100 mM CypK. For this purpose, weigh 64 mg of CypK, add 2.5 mL of 0.1 sodium hydroxide, vortex, spin down to recover all undissolved particles and sonicate.
3. Add 2.5 mL of CypK (100 mM in 0.1 M NaOH) to 42.5 mL of expression medium supplemented with antibiotics. Mix well, add 250 µL of 0.1 M HCl, and sterilize using a 0.22 µm filter.
4.Dilute 50 µg of HC and LC pKym1 plasmids to 2.5 mL with reduced serum medium. In a separate tube, dilute 135 µL of transfection reagent to 2.5 mL with reduced serum medium.
5. Five minutes after preparing the solutions, mix the plasmids and the transfection reagent solution and incubate for 20 min to allow the formation of complexes between the DNA and the transfection reagent.
6. In the meantime, centrifuge 125 million cells at the target density for 5 min at 500 x g, resuspend with the expression medium containing CypK and add the DNA–transfection reagent mixture.
7. After incubating cells for 20 h, add 250 µL of transfection reagent enhancers included in the kit.
8. Harvest antibodies from the supernatant 6-7 days after addition of CypK (no change of medium is required during expression).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

256.30

Formula

C₁₂H₂₀N₂O₄

CAS No.

1610703-09-7

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CCCCNC(OCC1C(C)=C1)=O)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 30 mg/mL (117.05 mM; ultrasonic and adjust pH to 2 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9017 mL	19.5084 mL	39.0168 mL
5 mM	0.7803 mL	3.9017 mL	7.8034 mL
10 mM	0.3902 mL	1.9508 mL	3.9017 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (11.71 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (11.71 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (11.71 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.30%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (243 KB) HNMR (458 KB) RP-HPLC (1362 KB)

Handling Instructions (2659 KB)

References

[1]. Oller-Salvia B. Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction. J Vis Exp. 2018 Sep 14;(139):58066. [Content Brief]

[2]. Oller-Salvia B, et, al. Rapid and Efficient Generation of Stable Antibody-Drug Conjugates via an Encoded Cyclopropene and an Inverse-Electron-Demand Diels-Alder Reaction. Angew Chem Int Ed Engl. 2018 Mar 5;57(11):2831-2834. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9017 mL	19.5084 mL	39.0168 mL	97.5419 mL
	5 mM	0.7803 mL	3.9017 mL	7.8034 mL	19.5084 mL
	10 mM	0.3902 mL	1.9508 mL	3.9017 mL	9.7542 mL
	15 mM	0.2601 mL	1.3006 mL	2.6011 mL	6.5028 mL
	20 mM	0.1951 mL	0.9754 mL	1.9508 mL	4.8771 mL
	25 mM	0.1561 mL	0.7803 mL	1.5607 mL	3.9017 mL
	30 mM	0.1301 mL	0.6503 mL	1.3006 mL	3.2514 mL
	40 mM	0.0975 mL	0.4877 mL	0.9754 mL	2.4385 mL
	50 mM	0.0780 mL	0.3902 mL	0.7803 mL	1.9508 mL
	60 mM	0.0650 mL	0.3251 mL	0.6503 mL	1.6257 mL
	80 mM	0.0488 mL	0.2439 mL	0.4877 mL	1.2193 mL
	100 mM	0.0390 mL	0.1951 mL	0.3902 mL	0.9754 mL

CypK Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CypK1610703-09-7N-Cyclopropene-L-LysineOtherscyclopropene derivativelysinegenetic-code expansionprotein conjugatesbioorthogonal handleInhibitorinhibitorinhibit

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CypK (Synonyms: N-Cyclopropene-L-Lysine)

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CypK Related Antibodies

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CypK Related Antibodies

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Complete Stock Solution Preparation Table

CypK Related Classifications

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