2. Metabolic Enzyme/Protease Anti-infection
  3. Mitochondrial Metabolism Bacterial
  4. D-Histidine

D-Histidine is an anti-biofilm agent that targets bacterial quorum sensing systems (such as RhlI/RhlR pathway) and has antibacterial activity. D-Histidine works by non-covalently binding to bacterial regulatory factors or copper ion complexes, selectively inhibiting bacterial biofilm formation and motility. D-Histidine downregulates quorum sensing-related gene expression, reduces the synthesis of virulence factors (such as alginate and proteases), and interferes with bacterial membrane stability, inhibiting biofilm formation, promoting the disintegration of mature biofilms, and enhancing antibiotic sensitivity. D-Histidine is also an efficient catalyst for the salt-induced peptide formation (SIPF) reaction, which promotes the condensation of amino acids to form dipeptides (such as dialanine and dilysine) by forming a complex with copper ions (Cu2+).

For research use only. We do not sell to patients.

D-Histidine Chemical Structure

D-Histidine Chemical Structure

CAS No. : 351-50-8

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Based on 1 publication(s) in Google Scholar

Other Forms of D-Histidine:

D-Histidine Related Antibodies

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Description

D-Histidine is an anti-biofilm agent that targets bacterial quorum sensing systems (such as RhlI/RhlR pathway) and has antibacterial activity. D-Histidine works by non-covalently binding to bacterial regulatory factors or copper ion complexes, selectively inhibiting bacterial biofilm formation and motility. D-Histidine downregulates quorum sensing-related gene expression, reduces the synthesis of virulence factors (such as alginate and proteases), and interferes with bacterial membrane stability, inhibiting biofilm formation, promoting the disintegration of mature biofilms, and enhancing antibiotic sensitivity. D-Histidine is also an efficient catalyst for the salt-induced peptide formation (SIPF) reaction, which promotes the condensation of amino acids to form dipeptides (such as dialanine and dilysine) by forming a complex with copper ions (Cu2+)[1][2][3].

In Vitro

D-Histidine (100 mM; 24 h) significantly inhibited biofilm formation and promoted the disintegration of mature biofilm in the Pseudomonas aeruginosa PAO1 biofilm formation experiment. Crystal violet staining and laser confocal microscopy showed that the amount of biofilm was reduced by about 55%[1].
D-Histidine (100 nM-100 mM; 24 h) downregulated RhlI/RhlR gene expression in a concentration-dependent manner in the quorum sensing (QS)-related gene expression experiment. At 100 mM, the expression levels of RhlI and RhlR genes decreased by about 80% and 90%, respectively[1].
D-Histidine (2.5 mM) increases the production of di-alanine by 14-22 times compared to the absence of catalyst; for the lysine system, D-Histidine increases the catalytic effect about 2-3 times[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

D-Histidine Related Antibodies

Cell Viability Assay[1]

Cell Line: Pseudomonas aeruginosa PAO1
Concentration: 0 mM, 100 nM, 10 µM, 1 mM, 50 mM, 100 mM
Incubation Time: 24 h
Result: High concentrations (50-100 mM) significantly reduced bacterial motility. Swarming motility diameter decreased by 68% and swimming motility by 81% at 100 mM, indicating impaired movement on semi-solid agar media.
In Vivo

Oxygen free radicals are associated with traumatic brain injury and brain edema (BE). For example, hydroxyl free radicals can induce lipid peroxidation, thereby generating lipid free radicals that may be an important source of singlet oxygen.
In the rat cryoinjury model, only L-histidine (HY-N0832) (100 mg/kg; intravenous injection; injection 30 min before injury) has singlet oxygen scavenger activity compared with D-histidine, and L-histidine reduces brain edema in rats after cryoinjury[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rats cryogenic injury model[3]
Dosage: Both L-histidine and D-histidine were 100 mg/kg
Administration: Intravenously at 30 minutes before lesion production
Result: Had no effect on rectal and brain temperature.
L-histidine but not D-histidine significantly attenuated BE following cryogenic injury.
Molecular Weight

155.16

Formula

C6H9N3O2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

OC([C@H](N)CC1=CN=CN1)=O
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 44.44 mg/mL (286.42 mM; ultrasonic and adjust pH to 7 with 1 M HCL)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.4451 mL 32.2254 mL 64.4508 mL
5 mM 1.2890 mL 6.4451 mL 12.8902 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.98%

SDS (393 KB)

COA (248 KB) RP-HPLC (202 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 6.4451 mL 32.2254 mL 64.4508 mL 161.1271 mL
5 mM 1.2890 mL 6.4451 mL 12.8902 mL 32.2254 mL
10 mM 0.6445 mL 3.2225 mL 6.4451 mL 16.1127 mL
15 mM 0.4297 mL 2.1484 mL 4.2967 mL 10.7418 mL
20 mM 0.3223 mL 1.6113 mL 3.2225 mL 8.0564 mL
25 mM 0.2578 mL 1.2890 mL 2.5780 mL 6.4451 mL
30 mM 0.2148 mL 1.0742 mL 2.1484 mL 5.3709 mL
40 mM 0.1611 mL 0.8056 mL 1.6113 mL 4.0282 mL
50 mM 0.1289 mL 0.6445 mL 1.2890 mL 3.2225 mL
60 mM 0.1074 mL 0.5371 mL 1.0742 mL 2.6855 mL
80 mM 0.0806 mL 0.4028 mL 0.8056 mL 2.0141 mL
100 mM 0.0645 mL 0.3223 mL 0.6445 mL 1.6113 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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D-Histidine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-Histidine351-50-8Mitochondrial MetabolismBacterialMitochondrialMetabolismEndogenousMetaboliteInhibitorinhibitorinhibit

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