2. Anti-infection Apoptosis
  3. HIV Apoptosis
  4. Dextran sulfate sodium salt (MW 4500-5500)

Dextran sulfate sodium salt (MW 4500-5500)  (Synonyms: DSS (MW 4500-5500); DXS (MW 4500-5500))

Cat. No.: HY-116282A
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Dextran sulfate sodium salt (MW 4500-5500) is a polymer of anhydroglucose with a molecular weight of 4500-5500. Dextran sulfate sodium salt (MW 4500-5500) can be used to induce acute colitis and cause apoptosis of colonic epithelial cells in mice. The recommended molecular weight in the study is 5000 (HY-116282) and the use concentration is 5% (in feed, W/W). The sulfated polysaccharide dextran sulfate is also an effective inhibitor of HIV. Dextran sulfate sodium salt (MW 4500-5500) can significantly inhibit HIV-1 replication at concentrations that do not significantly inhibit the blood coagulation process. Dextran sulfate sodium salt (MW 4500-5500) protects MT-4 cells from HIV-1-induced cellular pathogenicity. Dextran sulfate-induced colitis can be inhibited by Puerarin (HY-N0145), Baicalein (HY-N0196), β-Caryophyllene (HY-N1415).

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Dextran sulfate sodium salt (MW 4500-5500) Chemical Structure

Dextran sulfate sodium salt (MW 4500-5500) Chemical Structure

CAS No. : 9011-18-1

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Dextran sulfate sodium salt (MW 4500-5500) Related Antibodies

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Description

Dextran sulfate sodium salt (MW 4500-5500) is a polymer of anhydroglucose with a molecular weight of 4500-5500. Dextran sulfate sodium salt (MW 4500-5500) can be used to induce acute colitis and cause apoptosis of colonic epithelial cells in mice. The recommended molecular weight in the study is 5000 (HY-116282) and the use concentration is 5% (in feed, W/W). The sulfated polysaccharide dextran sulfate is also an effective inhibitor of HIV. Dextran sulfate sodium salt (MW 4500-5500) can significantly inhibit HIV-1 replication at concentrations that do not significantly inhibit the blood coagulation process. Dextran sulfate sodium salt (MW 4500-5500) protects MT-4 cells from HIV-1-induced cellular pathogenicity. Dextran sulfate-induced colitis can be inhibited by Puerarin (HY-N0145), Baicalein (HY-N0196), β-Caryophyllene (HY-N1415).

In Vitro

Take Dextran sulfate sodium salt (MW 5000) as an example. Dextran sulfate sodium salt (MW 5000) (25 μg/mL; 24 h) can completely protect MT-4 cells from HIV-1-induced cytopathogenicity[1].
Dextran sulfate sodium salt (DSS) was labeled with 2-aminopyridine (to obtain pyridylamino-DSS or PA-DSS) and used to detect its metabolism in Caco-2 cells. After treating Caco-2 cells with PA-DSS (MW 5000) (3%; 24 h), the cells were fixed with ethanol, permeabilized with acetone, and stained with 0.05% toluidine blue solution for 20 min. The results showed that:
Dextran sulfate sodium salt (MW 5000) reacted heterochromatically in the cells, and the color development results showed that it was mainly bound to the cell nucleus and depolymerized to below MW 2500 after entering the cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Dextran sulfate sodium salt (MW 4500-5500) Related Antibodies
In Vivo

Take Dextran sulfate sodium salt (MW 5000) as an example. Dextran sulfate sodium salt (MW 5000) induces inflammation primarily in the colon rather than in the stomach or small intestine. Dextran sulfate sodium salt (MW 5000) (3%, mixed in feed; fed for 8 days) induces colitis in mice, causing weight loss, mucosal damage, reduced cell mitotic rate, and induced apoptosis of colonic epithelial cells. Dextran sulfate sodium salt (MW 5000) is more effective than Dextran sulfate sodium salt (MW 2500) (statistically significant differences in all parameters were observed on day 6 after co-induction)[2].
Dextran sulfate sodium salt (average MW 5000) (3%, added to drinking water; gavage for 10 days) induces colitis in rats, causing blood in stool and decreased RBC and WBC indices[3].

Induction of Colitis[2][3][4][5]
Background
Adding dextran sulfate sodium (DSS) to the drinking water of mice or rats can induce colon epithelial damage and a strong inflammatory response that lasts for several days, inducing colitis[4]. Clinical manifestations of colitis typically include watery diarrhea, hematochezia, and weight loss. The induced symptoms are very similar to human ulcerative colitis (UC) and can be used to study the occurrence and development mechanisms of acute and chronic colitis. Models can be created in many species of animals: mice, rats, zebrafish, pigs, fruit flies, etc.
Specific Mmodeling Methods
Mice[2]: C57Bl/6n mice • male • specific pathogen-free • 6 week-old
Administration: 5% (W/W of diet) • po • 8 days
Rat[3]: SPF-grade Wistar rats • male • 7 weeks old
Administration: 3% w/v, 2 mL/kg • orally gavage (added in drinking water) • 10 days
Note
Dextran sulfate sodium salt (MW 5000) depolymerizes to MW 1800 molecules upon autoclaving (115°C, 15 min, 1.7 atm), with approximately 70% of the sulfate groups in each Dextran sulfate sodium salt molecule being depleted. Therefore, filter sterilization is recommended[2].


Modeling Indicators
Phenotypic observations: weight loss, blood in stool
Tissue level: increased mucosal injury score, increased white blood cell (WBC) count, decreased red blood cell (RBC) count
Cell level: cell division cycle arrest, decreased mitotic rate, apoptosis of colon epithelial cells
Correlated Product(s): Dextran sulfate sodium salt (MW 5000) (HY-116282); Dextran sulfate sodium salt (MW 35000-50000) (HY-116282C); Dextran sulfate sodium salt (MW 450000-550000) (HY-116282D); Oxazolone (HY-126360)
Opposite Product(s): Puerarin (HY-N0145)[5]; Monotropein (HY-N0648)[6]; β-Caryophyllene (HY-N1415)[7]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

4500-5500

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Dextran sulfate sodium salt (MW 4500-5500)]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (Need ultrasonic)

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (Infinity mM); Clear solution; Need ultrasonic

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This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
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Dextran sulfate sodium salt (MW 4500-5500) Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dextran sulfate sodium salt (MW 4500-5500)9011-18-1DSS (MW 4500-5500)DXS (MW 4500-5500)HIVApoptosisHuman immunodeficiency viruscolitishuman immunodeficiency virusInhibitorinhibitorinhibit

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