2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. Dihydrorhodamine 6G

Dihydrorhodamine 6G  (Synonyms: DHR 6G)

Cat. No.: HY-W247098 Purity: 98.17%
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Dihydrorhodamine 6G (DHR 6G) is the reduced form of Rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide.

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Dihydrorhodamine 6G Chemical Structure

Dihydrorhodamine 6G Chemical Structure

CAS No. : 217176-83-5

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Dihydrorhodamine 6G Related Antibodies

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Description

Dihydrorhodamine 6G (DHR 6G) is the reduced form of Rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide[1].

Molecular Weight

444.57

Formula

C28H32N2O3
CAS No.
Appearance

Solid

Color

Pale pink to pink

SMILES

O=C(OCC)C1=CC=CC=C1C2C3=C(OC4=C2C=C(C)C(NCC)=C4)C=C(NCC)C(C)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (112.47 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2494 mL 11.2468 mL 22.4936 mL
5 mM 0.4499 mL 2.2494 mL 4.4987 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2494 mL 11.2468 mL 22.4936 mL 56.2341 mL
5 mM 0.4499 mL 2.2494 mL 4.4987 mL 11.2468 mL
10 mM 0.2249 mL 1.1247 mL 2.2494 mL 5.6234 mL
15 mM 0.1500 mL 0.7498 mL 1.4996 mL 3.7489 mL
20 mM 0.1125 mL 0.5623 mL 1.1247 mL 2.8117 mL
25 mM 0.0900 mL 0.4499 mL 0.8997 mL 2.2494 mL
30 mM 0.0750 mL 0.3749 mL 0.7498 mL 1.8745 mL
40 mM 0.0562 mL 0.2812 mL 0.5623 mL 1.4059 mL
50 mM 0.0450 mL 0.2249 mL 0.4499 mL 1.1247 mL
60 mM 0.0375 mL 0.1874 mL 0.3749 mL 0.9372 mL
80 mM 0.0281 mL 0.1406 mL 0.2812 mL 0.7029 mL
100 mM 0.0225 mL 0.1125 mL 0.2249 mL 0.5623 mL
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Dihydrorhodamine 6G Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dihydrorhodamine 6G217176-83-5DHR 6GReactive Oxygen SpeciesInhibitorinhibitorinhibit

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