DOTA-tris(tBu)ester NHS ester

Name: DOTA-tris(tBu)ester NHS ester
Brand: MedChemExpress
SKU: HY-131326
Rating: 4.5 (1 reviews)

Cat. No.: HY-131326 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

DOTA-tris(tBu)ester NHS ester is a derivative of DOTA for the labeling of peptides and antibodies.

For research use only. We do not sell to patients.

DOTA-tris(tBu)ester NHS ester Chemical Structure

CAS No. : 819869-77-7

Size	Price	Stock	Quantity
25 mg	USD 98	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 156	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of DOTA-tris(tBu)ester NHS ester:

DOTA-NHS-ester Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

DOTA-tris(tBu)ester NHS ester is a derivative of DOTA for the labeling of peptides and antibodies^[1].

Molecular Weight

669.81

Formula

C₃₂H₅₅N₅O₁₀

CAS No.

819869-77-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(ON1C(CCC1=O)=O)CN2CCN(CC(OC(C)(C)C)=O)CCN(CC(OC(C)(C)C)=O)CCN(CC(OC(C)(C)C)=O)CC2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (149.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4930 mL	7.4648 mL	14.9296 mL
5 mM	0.2986 mL	1.4930 mL	2.9859 mL
10 mM	0.1493 mL	0.7465 mL	1.4930 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (253 KB) HNMR (269 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Wängler C, et, al. Improved syntheses and applicability of different DOTA building blocks for multiply derivatized scaffolds. Bioorg Med Chem. 2008 Mar 1; 16(5): 2606-16. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4930 mL	7.4648 mL	14.9296 mL	37.3240 mL
	5 mM	0.2986 mL	1.4930 mL	2.9859 mL	7.4648 mL
	10 mM	0.1493 mL	0.7465 mL	1.4930 mL	3.7324 mL
	15 mM	0.0995 mL	0.4977 mL	0.9953 mL	2.4883 mL
	20 mM	0.0746 mL	0.3732 mL	0.7465 mL	1.8662 mL
	25 mM	0.0597 mL	0.2986 mL	0.5972 mL	1.4930 mL
	30 mM	0.0498 mL	0.2488 mL	0.4977 mL	1.2441 mL
	40 mM	0.0373 mL	0.1866 mL	0.3732 mL	0.9331 mL
	50 mM	0.0299 mL	0.1493 mL	0.2986 mL	0.7465 mL
	60 mM	0.0249 mL	0.1244 mL	0.2488 mL	0.6221 mL
	80 mM	0.0187 mL	0.0933 mL	0.1866 mL	0.4666 mL
	100 mM	0.0149 mL	0.0746 mL	0.1493 mL	0.3732 mL