EGFR-IN-70

Name: EGFR-IN-70
Brand: MedChemExpress
SKU: HY-150611
Rating: 4.5 (1 reviews)

Cat. No.: HY-150611 Purity: 99.16%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

EGFR-IN-70 (compound 18j) is a potent EGFR inhibitor with IC₅₀ values of 23.6 and 307.5 nM for EGFR^LR/TM/CS and EGFR^WT, respectively. EGFR-IN-70 has anti-proliferative activity and suppresses phosphorylation of the EGFR. EGFR-IN-70 can be used for cancer research.

For research use only. We do not sell to patients.

EGFR-IN-70 Chemical Structure

CAS No. : 2926716-96-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 661	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 661	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2600	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4000	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All EGFR Isoform Specific Products:

View All Isoforms

EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC₅₀: 23.6 nM (EGFR^LR/TM/CS) and 307.5 nM EGFR^WT)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	2.003 nM Compound: 18j	Antiproliferative activity against human A-431 cells harboring wild type EGFR assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human A-431 cells harboring wild type EGFR assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 35446588]
BaF3	IC₅₀	0.036 μM Compound: 18j	Antiproliferative activity against mouse BaF3 cells stably expressing EGFR L858R/T790M/C797S mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Antiproliferative activity against mouse BaF3 cells stably expressing EGFR L858R/T790M/C797S mutant assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 35446588]
BaF3	IC₅₀	0.052 nM Compound: 18j	Antiproliferative activity against mouse BaF3 cells harboring EGFR 19 del/T790M/C797S mutant assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay Antiproliferative activity against mouse BaF3 cells harboring EGFR 19 del/T790M/C797S mutant assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay	[PMID: 35446588]
BaF3	IC₅₀	2.083 μM Compound: 18j	Cytotoxicity against mouse IL-3 dependent BaF3 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Cytotoxicity against mouse IL-3 dependent BaF3 cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 35446588]
PC-9	IC₅₀	0.644 nM Compound: 18j	Antiproliferative activity against human osimertinib-resistant PC-9 cells harboring EGFR 19 del/T790M/C797S mutant assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human osimertinib-resistant PC-9 cells harboring EGFR 19 del/T790M/C797S mutant assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 35446588]

In Vitro

EGFR-IN-70 (compound 18j) (72 hours) has anti-proliferative activity and suppresses the proliferation of Ba/F3-EGFR^19del/TM/CS, PC-9-OR-EGFR^19del/TM/CS and A431- EGFR^WT cells with IC₅₀ values of 0.052, 0.644 and 2.003 μM, respectively^[1].
EGFR-IN-70 (compound 18j) (0-1000 nM; 2 hours; Ba/F3-EGFR^19del/TM/CS and PC-9-OR-EGFR^19del/TM/CS cells) can suppresse phosphorylation of the EGFR^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Ba/F3-EGFR^19del/TM/CS and PC-9-OR-EGFR^19del/TM/CS cells
Concentration:	1, 3, 10, 30, 100, 300 and 1000 nM
Incubation Time:	2 hours
Result:	Suppressed phosphorylation of the EGFR and the downstream signaling ERK level.

Molecular Weight

626.17

Formula

C₃₁H₃₆ClN₅O₅S

CAS No.

2926716-96-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(CC)(N1C2=CC(OCCCCCCOC3=CC(N4)=C(C)C=C3N5CCOCC5)=CC=C2C(C6=NC4=NC=C6Cl)=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (159.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5970 mL	7.9851 mL	15.9701 mL
5 mM	0.3194 mL	1.5970 mL	3.1940 mL
10 mM	0.1597 mL	0.7985 mL	1.5970 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.16%

Select Batch:

Data Sheet (283 KB) SDS (251 KB)

COA (264 KB) HNMR (147 KB) RP-HPLC (165 KB) LCMS (388 KB)

Handling Instructions (2659 KB)

References

[1]. Chen H, et, al. Conformational Constrained 4-(1-Sulfonyl-3-indol)yl-2-phenylaminopyrimidine Derivatives as New Fourth-Generation Epidermal Growth Factor Receptor Inhibitors Targeting T790M/C797S Mutations. J Med Chem. 2022 May 12;65(9):6840-6858. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5970 mL	7.9851 mL	15.9701 mL	39.9253 mL
	5 mM	0.3194 mL	1.5970 mL	3.1940 mL	7.9851 mL
	10 mM	0.1597 mL	0.7985 mL	1.5970 mL	3.9925 mL
	15 mM	0.1065 mL	0.5323 mL	1.0647 mL	2.6617 mL
	20 mM	0.0799 mL	0.3993 mL	0.7985 mL	1.9963 mL
	25 mM	0.0639 mL	0.3194 mL	0.6388 mL	1.5970 mL
	30 mM	0.0532 mL	0.2662 mL	0.5323 mL	1.3308 mL
	40 mM	0.0399 mL	0.1996 mL	0.3993 mL	0.9981 mL
	50 mM	0.0319 mL	0.1597 mL	0.3194 mL	0.7985 mL
	60 mM	0.0266 mL	0.1331 mL	0.2662 mL	0.6654 mL
	80 mM	0.0200 mL	0.0998 mL	0.1996 mL	0.4991 mL
	100 mM	0.0160 mL	0.0799 mL	0.1597 mL	0.3993 mL

EGFR-IN-70 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EGFR-IN-702926716-96-1EGFREpidermal growth factor receptorErbB-1HER1anticancerphosphorylationEGFR LR/TM/CSEGFR WTInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

EGFR-IN-70

Customer Review

EGFR-IN-70 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All EGFR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

EGFR-IN-70 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

EGFR-IN-70 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report