Eosin 5-isothiocyanate

Name: Eosin 5-isothiocyanate
Brand: MedChemExpress
SKU: HY-D0805
Rating: 4.5 (1 reviews)

Cat. No.: HY-D0805 Purity: 95.02%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Eosin 5-isothiocyanate is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.

For research use only. We do not sell to patients.

Eosin 5-isothiocyanate Chemical Structure

CAS No. : 60520-47-0

Size	Price	Stock	Quantity
5 mg	USD 89	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 142	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Molecular Weight

704.97

Formula

C₂₁H₇Br₄NO₅S

CAS No.

60520-47-0

Appearance

Solid

Color

Orange to reddish brown

SMILES

O=C1OC2(C3=C(OC4=C2C=C(Br)C(O)=C4Br)C(Br)=C(O)C(Br)=C3)C5=C1C=C(N=C=S)C=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (70.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4185 mL	7.0925 mL	14.1850 mL
5 mM	0.2837 mL	1.4185 mL	2.8370 mL
10 mM	0.1419 mL	0.7093 mL	1.4185 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 95.02%

Select Batch:

Data Sheet (279 KB) SDS (419 KB)

COA (260 KB)

Handling Instructions (2659 KB)

References

[1]. Sultana M, et al. A review on experimental chemically modified activated carbon to enhance dye and heavy metals adsorption[J]. Cleaner engineering and technology, 2022, 6: 100382.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4185 mL	7.0925 mL	14.1850 mL	35.4625 mL
	5 mM	0.2837 mL	1.4185 mL	2.8370 mL	7.0925 mL
	10 mM	0.1419 mL	0.7093 mL	1.4185 mL	3.5463 mL
	15 mM	0.0946 mL	0.4728 mL	0.9457 mL	2.3642 mL
	20 mM	0.0709 mL	0.3546 mL	0.7093 mL	1.7731 mL
	25 mM	0.0567 mL	0.2837 mL	0.5674 mL	1.4185 mL
	30 mM	0.0473 mL	0.2364 mL	0.4728 mL	1.1821 mL
	40 mM	0.0355 mL	0.1773 mL	0.3546 mL	0.8866 mL
	50 mM	0.0284 mL	0.1419 mL	0.2837 mL	0.7093 mL
	60 mM	0.0236 mL	0.1182 mL	0.2364 mL	0.5910 mL