Ethylene glycol-d₄

Cat. No.: HY-Y0338S2 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ethylene glycol-d₄ is the deuterium labeled Ethylene glycol[1].

For research use only. We do not sell to patients.

Ethylene glycol-d<sub>4</sub> Chemical Structure

Ethylene glycol-d₄ Chemical Structure

CAS No. : 2219-51-4

Size	Price	Stock	Quantity
50 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 40	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
1 g	USD 190	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Other Forms of Ethylene glycol-d₄:

Ethylene glycol,98% Get quote

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Purity & Documentation
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Description

Ethylene glycol-d₄ is the deuterium labeled Ethylene glycol[1].

In Vitro

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

66.09

Formula

C₂H₂D₄O₂

CAS No.

2219-51-4

Unlabeled CAS

107-21-1

Appearance

Liquid (Density: 1.184 g/cm³)

Color

Colorless to light yellow

SMILES

OC([2H])([2H])C([2H])([2H])O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 200 mg/mL (3026.18 mM; Need ultrasonic)

DMSO : 100 mg/mL (1513.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	15.1309 mL	75.6544 mL	151.3088 mL
5 mM	3.0262 mL	15.1309 mL	30.2618 mL
10 mM	1.5131 mL	7.5654 mL	15.1309 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (37.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (37.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (37.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (273 KB) SDS (481 KB)

COA (250 KB) GC (176 KB)

Handling Instructions (2659 KB)

References

[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / DMSO / H₂O	1 mM	15.1309 mL	75.6544 mL	151.3088 mL	378.2721 mL
	5 mM	3.0262 mL	15.1309 mL	30.2618 mL	75.6544 mL
	10 mM	1.5131 mL	7.5654 mL	15.1309 mL	37.8272 mL
	15 mM	1.0087 mL	5.0436 mL	10.0873 mL	25.2181 mL
	20 mM	0.7565 mL	3.7827 mL	7.5654 mL	18.9136 mL
	25 mM	0.6052 mL	3.0262 mL	6.0524 mL	15.1309 mL
	30 mM	0.5044 mL	2.5218 mL	5.0436 mL	12.6091 mL
	40 mM	0.3783 mL	1.8914 mL	3.7827 mL	9.4568 mL
	50 mM	0.3026 mL	1.5131 mL	3.0262 mL	7.5654 mL
	60 mM	0.2522 mL	1.2609 mL	2.5218 mL	6.3045 mL
	80 mM	0.1891 mL	0.9457 mL	1.8914 mL	4.7284 mL
	100 mM	0.1513 mL	0.7565 mL	1.5131 mL	3.7827 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.