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  4. EtS-DMAB

EtS-DMAB  (Synonyms: HClO-green)

Cat. No.: HY-D1265 Purity: 99.80%
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EtS-DMAB (HClO-green) is a fluorescent probe, which can selectively detect hypochlorous acid (HOCl) (λex=440 nm, λem=610 nm). EtS-DMAB is applied to image exogenous and endogenous HOCl in live cells.

For research use only. We do not sell to patients.

EtS-DMAB Chemical Structure

EtS-DMAB Chemical Structure

CAS No. : 2929446-76-2

Size Price Stock Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 330 In-stock
Solution
10 mM * 1 mL in DMSO USD 330 In-stock
Solid or liquid
5 mg USD 300 In-stock
10 mg USD 480 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

EtS-DMAB Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE EtS-DMAB

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

EtS-DMAB (HClO-green) is a fluorescent probe, which can selectively detect hypochlorous acid (HOCl) (λex=440 nm, λem=610 nm). EtS-DMAB is applied to image exogenous and endogenous HOCl in live cells[1].

In Vitro

EtS-DMAB can selectively detect hypochlorous acid (HOCl) over other reactive oxygen species in aqueous solution with a large stokes shift (～170 nm). It turns out that oxidation of the thioether to the corresponding sulfoxide accounts for the turn-on fluorescence[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EtS-DMAB Related Antibodies
Molecular Weight

223.29

Formula

C10H13N3OS
CAS No.
Appearance

Viscous Liquid

Color

Brown to reddish brown

Emission (Em)

610
Excitation (Ex)

440
SMILES

CN(C1=CC=C(C2=NON=C21)SCC)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (223.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4785 mL 22.3924 mL 44.7848 mL
5 mM 0.8957 mL 4.4785 mL 8.9570 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (11.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (11.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4785 mL 22.3924 mL 44.7848 mL 111.9620 mL
5 mM 0.8957 mL 4.4785 mL 8.9570 mL 22.3924 mL
10 mM 0.4478 mL 2.2392 mL 4.4785 mL 11.1962 mL
15 mM 0.2986 mL 1.4928 mL 2.9857 mL 7.4641 mL
20 mM 0.2239 mL 1.1196 mL 2.2392 mL 5.5981 mL
25 mM 0.1791 mL 0.8957 mL 1.7914 mL 4.4785 mL
30 mM 0.1493 mL 0.7464 mL 1.4928 mL 3.7321 mL
40 mM 0.1120 mL 0.5598 mL 1.1196 mL 2.7991 mL
50 mM 0.0896 mL 0.4478 mL 0.8957 mL 2.2392 mL
60 mM 0.0746 mL 0.3732 mL 0.7464 mL 1.8660 mL
80 mM 0.0560 mL 0.2799 mL 0.5598 mL 1.3995 mL
100 mM 0.0448 mL 0.2239 mL 0.4478 mL 1.1196 mL
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EtS-DMAB Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EtS-DMAB2929446-76-2HClO-greenFluorescent DyeHOClfluorescentprobelivecellssulfoxideInhibitorinhibitorinhibit

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