Filipin III

Name: Filipin III
Brand: MedChemExpress
SKU: HY-N6718
Rating: 5.0 (5 reviews)

Cat. No.: HY-N6718 Purity: 98.39%: FAQs
Data Sheet SDS COA Handling Instructions

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Filipin III is the major component of Filipin, a 28-membered ring pentaene macrolide antifungal antibiotic produced by S. filipinensis, S. avermitilis and S. miharaensis. Filipin interacts with membrane sterols causing the alteration of membrane structure.

For research use only. We do not sell to patients.

Filipin III Chemical Structure

CAS No. : 480-49-9

Size	Price	Stock	Quantity
1 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 725	In-stock	Estimated Time of Arrival: December 31
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50 mg		Get quote

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Based on 5 publication(s) in Google Scholar

5 Publications Citing Use of MCE Filipin III

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Biological Activity
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References
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Description

IC₅₀ & Target

Macrolide

In Vitro

Cell staining:
Prepare Filipin III working solution in TBS-T. NCI-60 cancer cells were treated with 50 μg/mL Filipin III in the dark. After incubation for 1 hour at room temperature, cells were washed with TBS-T. Coverslips were then detached and mounted in a water-based mountant. Images were taken on an Olympus Provis fluorescence microscope equipped with a 40X oil objective, and quantification of cholesterol levels was accomplished using the particle size algorithm in MetaXpress image analysis software (Molecular Devices Corp., Sunnyvale CA)^[2].
Huang Y, et al assessed cholesterol enrichment on transsomes relative to TSPAN-GFP. NRK cells stably expressing TSPAN4-GFP were incubated with 2 μg/mL Filipin III at 37 ℃. After 15 minutes of treatment, Z-stack images were acquired using confocal microscopy to measure filipin III intensity on migrasomes (IpM), TSPAN4-GFP intensity on migrasomes (ItM), filipin III intensity on RFs (IpR), and TSPAN4- GFP on RF. Strength(ItR)^[3].
Or as Kwiatkowska K, et al exposed fibroblasts (Fibroblast) to 25 μg/ml Filipin III and incubated in the dark for 45 minutes (20 ℃), the staining results were obtained^[4].

Tissue staining:
Filipin binds to cholesterol with high affinity; however, it exhibits rapid photobleaching and produces only modest natural fluorescence upon UV excitation, making its detection under confocal microscopy difficult^[5].
Reid PC et al used Filipin III to detect cholesterol accumulation in neurons. NPC cerebellar tissue was stained with Filipin III (125 μg/ml) for 2 h at room temperature in the dark. After incubation together, slides were washed with 0.5 M Tris without FBS and counterstained with fluorescent dyes. On day 22, cholesterol accumulation was observed in Purkinje neuronal cells. Or staining the NPC cortex with Filipin III, cholesterol accumulation in the axon hillock region of most neurons was observed on day 22^[5].
Novak A et al use Filipin III to detect cholesterol accumulation in tumors. Dissolve Filipin III in DMSO/PBS (1:4) to make a working solution of 50 μg/mL. Subsequently, the tissue sections were incubated at room temperature for 30 min. After rinsing, the slides were mounted in an aqueous fluorescent mountant for subsequent observation^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

654.83

Formula

C₃₅H₅₈O₁₁

CAS No.

480-49-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1O[C@H](C)[C@@H](O)/C=C/C=C/C=C/C=C/C=C(C)/[C@@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)C[C@H](O)[C@H]1[C@H](O)CCCCC

Structure Classification

Initial Source

Microorganisms

Streptomyces miharaensis

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 10 mg/mL (15.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Methanol : 10 mg/mL (15.27 mM; Need ultrasonic and warming)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5271 mL	7.6356 mL	15.2711 mL
5 mM	0.3054 mL	1.5271 mL	3.0542 mL
10 mM	0.1527 mL	0.7636 mL	1.5271 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.0%

Select Batch:

Data Sheet (282 KB) SDS (251 KB)

COA (255 KB) HNMR (312 KB) RP-HPLC (128 KB)

Handling Instructions (2659 KB)

References

[1]. Payero TD, et al. Functional analysis of filipin tailoring genes from Streptomyces filipinensis reveals alternative routes in filipin III biosynthesis and yields bioactive derivatives. Microb Cell Fact. 2015 Aug 7;14:114. [Content Brief]

[2]. Warita K, et al. Statin-induced mevalonate pathway inhibition attenuates the growth of mesenchymal-like cancer cells that lack functional E-cadherin mediated cell cohesion. Sci Rep. 2014 Dec 23;4:7593. [Content Brief]

[3]. Huang Y, et al. Migrasome formation is mediated by assembly of micron-scale tetraspanin macrodomains. Nat Cell Biol. 2019 Aug;21(8):991-1002. [Content Brief]

[4]. Kwiatkowska K, et al. Visualization of cholesterol deposits in lysosomes of Niemann-Pick type C fibroblasts using recombinant perfringolysin O. Orphanet J Rare Dis. 2014 Apr 28;9:64. [Content Brief]

[5]. Reid PC, et al. A novel cholesterol stain reveals early neuronal cholesterol accumulation in the Niemann-Pick type C1 mouse brain. J Lipid Res. 2004 Mar;45(3):582-91. [Content Brief]

[6]. Novak A, et al. Role of the membrane cholesterol-glycosphingolipid complex as a ‘transistor’to regulate GSL receptor function and signaling of both lipids[J]. bioRxiv, 2017: 137612.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Methanol	1 mM	1.5271 mL	7.6356 mL	15.2711 mL	38.1778 mL
	5 mM	0.3054 mL	1.5271 mL	3.0542 mL	7.6356 mL
	10 mM	0.1527 mL	0.7636 mL	1.5271 mL	3.8178 mL
	15 mM	0.1018 mL	0.5090 mL	1.0181 mL	2.5452 mL

Filipin III Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Filipin III480-49-9AntibioticFungalInhibitorinhibitorinhibit

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Filipin III

Customer Review

Filipin III Related Antibodies

5 Publications Citing Use of MCE Filipin III

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Filipin III Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Filipin III Related Classifications

Keywords:

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