Fmoc-NH-PEG4-CH2CH2COOH (Synonyms: Fmoc-15-amino-4,7,10,13-tetraoxapentadecanoic acid)

Name: Fmoc-NH-PEG4-CH2CH2COOH
Brand: MedChemExpress
SKU: HY-W000434
Rating: 4.5 (1 reviews)

Cat. No.: HY-W000434 Purity: 99.92%: FAQs
Data Sheet SDS COA Handling Instructions

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Fmoc-NH-PEG4-CH2CH2COOH is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). Fmoc-NH-PEG4-CH2CH2COOH is also a PEG-based PROTAC linker that can be used in the synthesis of PROTACs.

For research use only. We do not sell to patients.

Fmoc-NH-PEG4-CH2CH2COOH Chemical Structure

CAS No. : 557756-85-1

Size	Price	Stock	Quantity
100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Based on 1 publication(s) in Google Scholar

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PEG Linkers Alkyl-Chain Linkers Alkyl/ether Linkers

Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

Cleavable Linker

PEGs

In Vitro

ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker.
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

487.54

Formula

C₂₆H₃₃NO₈

CAS No.

557756-85-1

Appearance

Liquid (Density: 1.219 g/cm³)

Color

Colorless to light yellow

SMILES

O=C(OCC1C2=C(C3=C1C=CC=C3)C=CC=C2)NCCOCCOCCOCCOCCC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (205.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0511 mL	10.2556 mL	20.5111 mL
5 mM	0.4102 mL	2.0511 mL	4.1022 mL
10 mM	0.2051 mL	1.0256 mL	2.0511 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (268 KB) SDS (393 KB)

COA (243 KB) HNMR (278 KB) LCMS (109 KB)

Handling Instructions (2659 KB)

References

[1]. Cai P, et al. Synthesis of C3-Alkylated Indoles on DNA via Indolyl Alcohol Formation Followed by Metal-FreeTransfer Hydrogenation. Org Lett. 2019 Aug 14. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0511 mL	10.2556 mL	20.5111 mL	51.2778 mL
	5 mM	0.4102 mL	2.0511 mL	4.1022 mL	10.2556 mL
	10 mM	0.2051 mL	1.0256 mL	2.0511 mL	5.1278 mL
	15 mM	0.1367 mL	0.6837 mL	1.3674 mL	3.4185 mL
	20 mM	0.1026 mL	0.5128 mL	1.0256 mL	2.5639 mL
	25 mM	0.0820 mL	0.4102 mL	0.8204 mL	2.0511 mL
	30 mM	0.0684 mL	0.3419 mL	0.6837 mL	1.7093 mL
	40 mM	0.0513 mL	0.2564 mL	0.5128 mL	1.2819 mL
	50 mM	0.0410 mL	0.2051 mL	0.4102 mL	1.0256 mL
	60 mM	0.0342 mL	0.1709 mL	0.3419 mL	0.8546 mL
	80 mM	0.0256 mL	0.1282 mL	0.2564 mL	0.6410 mL
	100 mM	0.0205 mL	0.1026 mL	0.2051 mL	0.5128 mL

Fmoc-NH-PEG4-CH2CH2COOH Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fmoc-NH-PEG4-CH2CH2COOH557756-85-1Fmoc-15-amino-4,7,10,13-tetraoxapentadecanoic acidADC LinkerPROTAC LinkersAntibody-drug conjugates linkerInhibitorinhibitorinhibit

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Fmoc-NH-PEG4-CH2CH2COOH (Synonyms: Fmoc-15-amino-4,7,10,13-tetraoxapentadecanoic acid)

Customer Review

Fmoc-NH-PEG4-CH2CH2COOH Related Antibodies

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•Related Small Molecules:

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View All ADC Linker Isoform Specific Products:

View All PROTAC Linkers Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Fmoc-NH-PEG4-CH2CH2COOH Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

Fmoc-NH-PEG4-CH2CH2COOH Related Classifications

Keywords:

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Customer Review

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