Fmoc-OSu (Synonyms: N-(Fmoc-oxy)succinimide)

Name: Fmoc-OSu
Brand: MedChemExpress
SKU: HY-79647
Rating: 4.5 (1 reviews)

Cat. No.: HY-79647 Purity: 99.72%: FAQs
Data Sheet SDS COA Handling Instructions

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N-(9-Fluorenylmethoxycarbonyloxy)succinimide is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

Fmoc-OSu Chemical Structure

CAS No. : 82911-69-1

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Description

N-(9-Fluorenylmethoxycarbonyloxy)succinimide is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

Molecular Weight

337.33

Formula

C₁₉H₁₅NO₅

CAS No.

82911-69-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1OC(OCC2C3=CC=CC=C3C4=CC=CC=C24)=O)CCC1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (296.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9645 mL	14.8223 mL	29.6446 mL
5 mM	0.5929 mL	2.9645 mL	5.9289 mL
10 mM	0.2964 mL	1.4822 mL	2.9645 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (249 KB) SDS (597 KB)

COA (257 KB) HNMR (116 KB) RP-HPLC (185 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9645 mL	14.8223 mL	29.6446 mL	74.1114 mL
	5 mM	0.5929 mL	2.9645 mL	5.9289 mL	14.8223 mL
	10 mM	0.2964 mL	1.4822 mL	2.9645 mL	7.4111 mL
	15 mM	0.1976 mL	0.9882 mL	1.9763 mL	4.9408 mL
	20 mM	0.1482 mL	0.7411 mL	1.4822 mL	3.7056 mL
	25 mM	0.1186 mL	0.5929 mL	1.1858 mL	2.9645 mL
	30 mM	0.0988 mL	0.4941 mL	0.9882 mL	2.4704 mL
	40 mM	0.0741 mL	0.3706 mL	0.7411 mL	1.8528 mL
	50 mM	0.0593 mL	0.2964 mL	0.5929 mL	1.4822 mL
	60 mM	0.0494 mL	0.2470 mL	0.4941 mL	1.2352 mL
	80 mM	0.0371 mL	0.1853 mL	0.3706 mL	0.9264 mL
	100 mM	0.0296 mL	0.1482 mL	0.2964 mL	0.7411 mL

Fmoc-OSu Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fmoc-OSu82911-69-1N-(Fmoc-oxy)succinimideBiochemical Assay Reagentsbiochemical reagentbiological materialorganic compoundlife scienceInhibitorinhibitorinhibit

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Fmoc-OSu (Synonyms: N-(Fmoc-oxy)succinimide)

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Complete Stock Solution Preparation Table

Fmoc-OSu Related Classifications

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