2. Epigenetics PI3K/Akt/mTOR Anti-infection
  3. AMPK Bacterial
  4. Galegine hydrochloride

Galegine hydrochloride, a guanidine derivative, contributes to weight loss in mice. Guanidine hydrochloride is the compound derived from G. officinalis, which gave rise to the biguanides, metformin and phenformin. Galegine hydrochloride activates AMPK in 3T3-L1 adipocytes and L6 myotubes, as well as in the H4IIE rat hepatoma and HEK293 human kidney cell lines. Galegine hydrochloride has antibacterial activity, with minimum inhibitory concentration of 4 mg/L against Staphylococcus aureus strains.

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Galegine hydrochloride Chemical Structure

Galegine hydrochloride Chemical Structure

CAS No. : 2368870-39-5

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Galegine hydrochloride Related Antibodies

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Description

Galegine hydrochloride, a guanidine derivative, contributes to weight loss in mice. Guanidine hydrochloride is the compound derived from G. officinalis, which gave rise to the biguanides, metformin and phenformin. Galegine hydrochloride activates AMPK in 3T3-L1 adipocytes and L6 myotubes, as well as in the H4IIE rat hepatoma and HEK293 human kidney cell lines. Galegine hydrochloride has antibacterial activity, with minimum inhibitory concentration of 4 mg/L against Staphylococcus aureus strains[1][2].

In Vitro

Pre-treatment with Galegine hydrochloride (10 μM-3 mM; 5 h) produces a concentration-dependent stimulation of insulin-independent glucose uptake by 3T3-L1 adipocytes without any effect on cell viability. Incubation with Galegine hydrochloride (1 μM-1 mM, 5 h) produces a concentration-dependent stimulation of glucose uptake into L6 myotubes, again without any effect on cell viability[1].
Galegine hydrochloride (0.3-300 μM; 24 hours) produced a slight reduction in basal glycerol release and a more marked reduction in isoprenaline-stimulated glycerol release from 3T3-L1 adipocytes. Incubation of H4IIE rat hepatoma cells with Galegine hydrochloride (10 or 300 μM) for up to 6 hours produces a time-dependent activation of AMPK measured in cell lysates, with maximal activation at 360 min and twofold activation still evident at 24 hous in the presence of 300 μM Galegine hydrochloride. The effect of 300 ΜM Galegine hydrochloride is markedly greater than that of 10 μM. Incubation with Galegine hydrochloride for 1 hour produces a concentration-dependent activation of AMPK in both 3T3L-1 adipocytes and L-6 myotubes. Galegine hydrochloride also produces a concentration-dependent activation of the enzyme in a human kidney cell line (HEK293)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Galegine hydrochloride Related Antibodies
In Vivo

Galegine hydrochloride (63 mg/kg; feed; daily for 11 days) produces a significant reduction in body weight[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult male BALB/c mice[1]
Dosage: 63 mg/kg
Administration: feed; daily for 11 days
Result: Produced a significant reduction in body weight.
Molecular Weight

163.65

Formula

C6H14ClN3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC(NC/C=C(C)\C)=N.[H]Cl
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 43.33 mg/mL (264.77 mM; Need ultrasonic)

DMSO : 5.2 mg/mL (31.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1106 mL 30.5530 mL 61.1060 mL
5 mM 1.2221 mL 6.1106 mL 12.2212 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 6.1106 mL 30.5530 mL 61.1060 mL 152.7651 mL
5 mM 1.2221 mL 6.1106 mL 12.2212 mL 30.5530 mL
10 mM 0.6111 mL 3.0553 mL 6.1106 mL 15.2765 mL
15 mM 0.4074 mL 2.0369 mL 4.0737 mL 10.1843 mL
20 mM 0.3055 mL 1.5277 mL 3.0553 mL 7.6383 mL
25 mM 0.2444 mL 1.2221 mL 2.4442 mL 6.1106 mL
30 mM 0.2037 mL 1.0184 mL 2.0369 mL 5.0922 mL
H2O 40 mM 0.1528 mL 0.7638 mL 1.5277 mL 3.8191 mL
50 mM 0.1222 mL 0.6111 mL 1.2221 mL 3.0553 mL
60 mM 0.1018 mL 0.5092 mL 1.0184 mL 2.5461 mL
80 mM 0.0764 mL 0.3819 mL 0.7638 mL 1.9096 mL
100 mM 0.0611 mL 0.3055 mL 0.6111 mL 1.5277 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Galegine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Galegine2368870-39-5AMPKBacterialAMP-activated protein kinaseguanidineweightloss3T3-L1adipocytesL6myotubesHEK293cellsantibacterialInhibitorinhibitorinhibit

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