GPR109 receptor agonist-1

Name: GPR109 receptor agonist-1
Brand: MedChemExpress
SKU: HY-107580
Rating: 4.5 (1 reviews)

Cat. No.: HY-107580 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

GPR109 receptor agonist-1 (Compound 3a) is a highly selective agonist of the human orphan G-protein-coupled receptor GPR109b, with the pEC₅₀ of 6.4. GPR109 receptor agonist-1 can be used for the research of cardio-metabolic diseases.

For research use only. We do not sell to patients.

GPR109 receptor agonist-1 Chemical Structure

CAS No. : 306935-41-1

Size	Price	Stock	Quantity
5 mg	USD 35	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 145	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Purity & Documentation
References
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Description

IC₅₀ & Target

pEC₅₀: 6.40 ± 0.36 (GPR109b)^[1]

Molecular Weight

205.21

Formula

C₁₀H₁₁N₃O₂

CAS No.

306935-41-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C1=CC=C(N(C(C)C)N=N2)C2=C1)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (487.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8731 mL	24.3653 mL	48.7306 mL
5 mM	0.9746 mL	4.8731 mL	9.7461 mL
10 mM	0.4873 mL	2.4365 mL	4.8731 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (248 KB) HNMR (392 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Graeme Semple, et al. 1-Alkyl-benzotriazole-5-carboxylic acids are highly selective agonists of the human orphan G-protein-coupled receptor GPR109b. J Med Chem. 2006 Feb 23;49(4):1227-30. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.8731 mL	24.3653 mL	48.7306 mL	121.8264 mL
	5 mM	0.9746 mL	4.8731 mL	9.7461 mL	24.3653 mL
	10 mM	0.4873 mL	2.4365 mL	4.8731 mL	12.1826 mL
	15 mM	0.3249 mL	1.6244 mL	3.2487 mL	8.1218 mL
	20 mM	0.2437 mL	1.2183 mL	2.4365 mL	6.0913 mL
	25 mM	0.1949 mL	0.9746 mL	1.9492 mL	4.8731 mL
	30 mM	0.1624 mL	0.8122 mL	1.6244 mL	4.0609 mL
	40 mM	0.1218 mL	0.6091 mL	1.2183 mL	3.0457 mL
	50 mM	0.0975 mL	0.4873 mL	0.9746 mL	2.4365 mL
	60 mM	0.0812 mL	0.4061 mL	0.8122 mL	2.0304 mL
	80 mM	0.0609 mL	0.3046 mL	0.6091 mL	1.5228 mL
	100 mM	0.0487 mL	0.2437 mL	0.4873 mL	1.2183 mL