GRL-1720

Cat. No.: HY-153533: FAQs
Data Sheet Handling Instructions

Molarity Calculator

GRL-1720 is a potent SARS-CoV-2 Mpro inhibitor with an EC₅₀ value of 15 µM. GRL-1720 shows anti-SARS-CoV2 activity.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (GRL-1720 TFA) that retains the same biological activity.

For research use only. We do not sell to patients.

GRL-1720 Chemical Structure

CAS No. : 2835511-03-8

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MOQ		Minimum order quantity
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This product is a controlled substance and not for sale in your territory.

Other Forms of GRL-1720:

GRL-1720 TFA Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

GRL-1720 is a potent SARS-CoV-2 Mpro inhibitor with an EC₅₀ value of 15 µM. GRL-1720 shows anti-SARS-CoV2 activity^[1].

IC₅₀ & Target

EC₅₀: 15 µM (SARS-CoV-2 Mpro)^[1]

In Vitro

GRL-1720 (1, 10, 100 µM; 3 days) shows protected activity against VeroE6 cells cultured with 100 µM SARS-CoV-2WK-521^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

274.70

Formula

C₁₄H₁₁ClN₂O₂

CAS No.

2835511-03-8

SMILES

O=C(C1=C2CCNC2=CC=C1)OC3=CC(Cl)=CN=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (364.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6403 mL	18.2017 mL	36.4033 mL
5 mM	0.7281 mL	3.6403 mL	7.2807 mL
10 mM	0.3640 mL	1.8202 mL	3.6403 mL

View the Complete Stock Solution Preparation Table

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.10 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.10 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (9.10 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hattori SI, et al. A small molecule compound with an indole moiety inhibits the main protease of SARS-CoV-2 and blocks virus replication. Nat Commun. 2021 Jan 28;12(1):668. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6403 mL	18.2017 mL	36.4033 mL	91.0084 mL
	5 mM	0.7281 mL	3.6403 mL	7.2807 mL	18.2017 mL
	10 mM	0.3640 mL	1.8202 mL	3.6403 mL	9.1008 mL
	15 mM	0.2427 mL	1.2134 mL	2.4269 mL	6.0672 mL
	20 mM	0.1820 mL	0.9101 mL	1.8202 mL	4.5504 mL
	25 mM	0.1456 mL	0.7281 mL	1.4561 mL	3.6403 mL
	30 mM	0.1213 mL	0.6067 mL	1.2134 mL	3.0336 mL
	40 mM	0.0910 mL	0.4550 mL	0.9101 mL	2.2752 mL
	50 mM	0.0728 mL	0.3640 mL	0.7281 mL	1.8202 mL
	60 mM	0.0607 mL	0.3034 mL	0.6067 mL	1.5168 mL
	80 mM	0.0455 mL	0.2275 mL	0.4550 mL	1.1376 mL
	100 mM	0.0364 mL	0.1820 mL	0.3640 mL	0.9101 mL

GRL-1720 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GRL-17202835511-03-8GRL1720GRL 1720SARS-CoVSARS coronavirusInhibitorinhibitorinhibit

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