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HaXS8 is a dimerizer that can promote a covalent and irreversible intracellular dimerization of HaloTag and SNAP-tagged proteins of interest. HaXS8 does not interfere with PI3K/mTOR signaling.

For research use only. We do not sell to patients.

HaXS8 Chemical Structure

HaXS8 Chemical Structure

CAS No. : 2080306-25-6

Size Price Stock Quantity
1 mg USD 260 In-stock
2 mg USD 390 In-stock
5 mg USD 780 Get quote
10 mg USD 1250 Get quote
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

HaXS8 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HaXS8 is a dimerizer that can promote a covalent and irreversible intracellular dimerization of HaloTag and SNAP-tagged proteins of interest. HaXS8 does not interfere with PI3K/mTOR signaling[1][2].

In Vitro

HaXS8 promotes intracellular dimerization of Halo-GFP and SNAP-GFP fusion proteins reached >65%, and displays significant intracellular dimerization as low as 50 nM in HeLa cells[1].
HaXS8 (0.5 μM; 40 min) induces a rapid and efficient cross-linking of the membrane anchor and the iSH2 construct, and triggers the activation of the downstream targets PKB/Akt and mTOR in HEK293 cells[1].
HaXS8 (0.5 μM; 40 min) does not upregulate MAPK phosphorylation in HEK293 cells[1].
HaXS8 (0.5 μM; 1 h) does not interfere with PI3K/mTOR signaling in HEK293 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HaXS8 Related Antibodies
Molecular Weight

787.20

Formula

C35H43ClF4N6O8
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=NC2=C(C(OCC3=CC=C(C=C3)CNC(COC4=C(C(F)=C(C(F)=C4F)OCCOCCOCCOCCOCCCCCCCl)F)=O)=N1)N=CN2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (63.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2703 mL 6.3516 mL 12.7033 mL
5 mM 0.2541 mL 1.2703 mL 2.5407 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (1.59 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.25 mg/mL (1.59 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2703 mL 6.3516 mL 12.7033 mL 31.7581 mL
5 mM 0.2541 mL 1.2703 mL 2.5407 mL 6.3516 mL
10 mM 0.1270 mL 0.6352 mL 1.2703 mL 3.1758 mL
15 mM 0.0847 mL 0.4234 mL 0.8469 mL 2.1172 mL
20 mM 0.0635 mL 0.3176 mL 0.6352 mL 1.5879 mL
25 mM 0.0508 mL 0.2541 mL 0.5081 mL 1.2703 mL
30 mM 0.0423 mL 0.2117 mL 0.4234 mL 1.0586 mL
40 mM 0.0318 mL 0.1588 mL 0.3176 mL 0.7940 mL
50 mM 0.0254 mL 0.1270 mL 0.2541 mL 0.6352 mL
60 mM 0.0212 mL 0.1059 mL 0.2117 mL 0.5293 mL
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HaXS8 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HaXS82080306-25-6HaXS 8HaXS-8OthersdimerizerdimerizationHalo,TagSNAP-taggedInhibitorinhibitorinhibit

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