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  2. Fluorescent Dye
  3. HBC

HBC is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence. HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC is a powerful tool for the study of intracellular RNA.

For research use only. We do not sell to patients.

HBC Chemical Structure

HBC Chemical Structure

CAS No. : 156840-13-0

Size Price Stock Quantity
1 mg USD 110 In-stock
5 mg USD 180 In-stock
10 mg USD 252 In-stock
25 mg USD 428 In-stock
50 mg USD 599 In-stock
100 mg USD 838 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

HBC is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence. HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC is a powerful tool for the study of intracellular RNA[1].

Clinical Trial
Molecular Weight

303.36

Formula

C19H17N3O

CAS No.
Appearance

Solid

Color

Orange to red

Emission (Em)

530

Excitation (Ex)

485

SMILES

N#CC1=CC=C(/C(C#N)=C\C2=CC=C(N(CCO)C)C=C2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (412.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2964 mL 16.4821 mL 32.9641 mL
5 mM 0.6593 mL 3.2964 mL 6.5928 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.69%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2964 mL 16.4821 mL 32.9641 mL 82.4103 mL
5 mM 0.6593 mL 3.2964 mL 6.5928 mL 16.4821 mL
10 mM 0.3296 mL 1.6482 mL 3.2964 mL 8.2410 mL
15 mM 0.2198 mL 1.0988 mL 2.1976 mL 5.4940 mL
20 mM 0.1648 mL 0.8241 mL 1.6482 mL 4.1205 mL
25 mM 0.1319 mL 0.6593 mL 1.3186 mL 3.2964 mL
30 mM 0.1099 mL 0.5494 mL 1.0988 mL 2.7470 mL
40 mM 0.0824 mL 0.4121 mL 0.8241 mL 2.0603 mL
50 mM 0.0659 mL 0.3296 mL 0.6593 mL 1.6482 mL
60 mM 0.0549 mL 0.2747 mL 0.5494 mL 1.3735 mL
80 mM 0.0412 mL 0.2060 mL 0.4121 mL 1.0301 mL
100 mM 0.0330 mL 0.1648 mL 0.3296 mL 0.8241 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
HBC
Cat. No.:
HY-D1373
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