2. Stem Cell/Wnt
  3. Hedgehog
  4. Hh-Ag1.5

Hh-Ag1.5 (SAg1.5) is a potent Hedgehog (Hh) agonist with an EC50 of 1 nM. Hh-Ag1.5 mediated reprogramming breaks the quiescence of noninjured liver stem cells for rescuing liver failure.

For research use only. We do not sell to patients.

Hh-Ag1.5 Chemical Structure

Hh-Ag1.5 Chemical Structure

CAS No. : 612542-14-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 451 In-stock
Solution
10 mM * 1 mL in DMSO USD 451 In-stock
Solid
1 mg USD 156 In-stock
5 mg USD 390 In-stock
10 mg USD 600 In-stock
25 mg USD 995 In-stock
50 mg USD 1400 In-stock
100 mg USD 1960 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Hh-Ag1.5 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Hh-Ag1.5

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Hh-Ag1.5 (SAg1.5) is a potent Hedgehog (Hh) agonist with an EC50 of 1 nM[1]. Hh-Ag1.5 mediated reprogramming breaks the quiescence of noninjured liver stem cells for rescuing liver failure[2].

IC50 & Target

EC50: 1 nM (Hedgehog)[1]
In Vitro

HhAg1.5 (5 μM; for 3 weeks) facilitates dramatic expansion of quiescent CD133+ CD45- liver stem cells (Hh3A cells) enriched from non-injured liver in vitro[2].
Hh-Ag1.5 competely binds to the Smo-containing membranes with the Ki value of 0.52 nM[3].
The Smo agonist SAG-1.5 displays nanomolar-range potency stimulation of Gli-responsive reporter activity, with an EC50 of 7 nM[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hh-Ag1.5 Related Antibodies
Molecular Weight

526.04

Formula

C28H26ClF2N3OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C(SC1=C(C=C2)F)=C(C1=C2F)Cl)N([C@@H](CC3)CC[C@H]3NC)CC4=CC(C5=CC=NC=C5)=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (95.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9010 mL 9.5050 mL 19.0100 mL
5 mM 0.3802 mL 1.9010 mL 3.8020 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.75 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.90%

COA (251 KB) LCMS (96 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9010 mL 9.5050 mL 19.0100 mL 47.5249 mL
5 mM 0.3802 mL 1.9010 mL 3.8020 mL 9.5050 mL
10 mM 0.1901 mL 0.9505 mL 1.9010 mL 4.7525 mL
15 mM 0.1267 mL 0.6337 mL 1.2673 mL 3.1683 mL
20 mM 0.0950 mL 0.4752 mL 0.9505 mL 2.3762 mL
25 mM 0.0760 mL 0.3802 mL 0.7604 mL 1.9010 mL
30 mM 0.0634 mL 0.3168 mL 0.6337 mL 1.5842 mL
40 mM 0.0475 mL 0.2376 mL 0.4752 mL 1.1881 mL
50 mM 0.0380 mL 0.1901 mL 0.3802 mL 0.9505 mL
60 mM 0.0317 mL 0.1584 mL 0.3168 mL 0.7921 mL
80 mM 0.0238 mL 0.1188 mL 0.2376 mL 0.5941 mL
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Hh-Ag1.5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hh-Ag1.5612542-14-0SAg1.5HedgehogquiescenceliverstemfailureInhibitorinhibitorinhibit

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