Humanin

Name: Humanin
Brand: MedChemExpress
SKU: HY-P1928
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1928 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Humanin, an anti-apoptotic peptide of 24 amino acids, is a Bax inhibitor. Humanin prevents the translocation of Bax from cytosol to mitochondria, blocks Bax from the inactive to active conformation. Humanin is a mitochondria-associated peptide with a neuroprotective effect against AD-related neurotoxicity. Humanin also improves overall insulin sensitivity in animal. Humanin are related to aging.
Humanin analogue, in which the serine at position 14 is replaced by glycine, names HNG.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Humanin Chemical Structure

CAS No. : 330936-69-1

Size	Price	Stock	Quantity
5 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 385	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 770	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Humanin, an anti-apoptotic peptide of 24 amino acids, is a Bax inhibitor. Humanin prevents the translocation of Bax from cytosol to mitochondria, blocks Bax from the inactive to active conformation. Humanin is a mitochondria-associated peptide with a neuroprotective effect against AD-related neurotoxicity. Humanin also improves overall insulin sensitivity in animal. Humanin are related to aging^[1]^[2].
Humanin analogue, in which the serine at position 14 is replaced by glycine, names HNG^[2].

IC₅₀ & Target^[1]

Bax

In Vitro

Humanin (40 nM, rhodamine-conjugated; 30 min) inhibits cell death induced by Bax protein in wild-type HCT116 cells^[1].
Humanin (100 μM; 10 min) blocks Bax association with isolated mitochondria, and suppresses cytochrome c release in wild-type HCT116 cells^[1].
Humanin reduces half apoptosis induced by 0.2 mM STS^[1].
Humanin fails to suppress apoptosis in Bax-deficient HCT116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Humanin (0.375 mg/kg/hr; i.v.) increases peripheral insulin sensitivity in Zucker diabetic fatty (ZDF) rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2687.23

Formula

C_{1₁₉H₂₀4}N₃₄O₃₂S₂

CAS No.

330936-69-1

Appearance

Solid

Color

White to off-white

Sequence

Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala

Sequence Shortening

MAPRGFSCLLLLTSEIDLPVKRRA

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (37.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (18.61 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.3721 mL	1.8607 mL	3.7213 mL
5 mM	0.0744 mL	0.3721 mL	0.7443 mL
10 mM	0.0372 mL	0.1861 mL	0.3721 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 10 mg/mL (3.72 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 10 mg/mL (3.72 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 10 mg/mL (3.72 mM); Clear solution

This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (242 KB) RP-HPLC (254 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Guo B, et al. Humanin peptide suppresses apoptosis by interfering with Bax activation. Nature. 2003 May 22;423(6938):456-61. [Content Brief]

[2]. Muzumdar RH, et al. Humanin: a novel central regulator of peripheral insulin action. PLoS One. 2009 Jul 22;4(7):e6334. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.3721 mL	1.8607 mL	3.7213 mL	9.3033 mL
	5 mM	0.0744 mL	0.3721 mL	0.7443 mL	1.8607 mL
	10 mM	0.0372 mL	0.1861 mL	0.3721 mL	0.9303 mL
	15 mM	0.0248 mL	0.1240 mL	0.2481 mL	0.6202 mL
DMSO	20 mM	0.0186 mL	0.0930 mL	0.1861 mL	0.4652 mL
	25 mM	0.0149 mL	0.0744 mL	0.1489 mL	0.3721 mL
	30 mM	0.0124 mL	0.0620 mL	0.1240 mL	0.3101 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Humanin Related Classifications

Neurological Disease Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Humanin330936-69-1Bcl-2 FamilyInhibitorinhibitorinhibit

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Humanin

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Humanin Related Antibodies

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•Related Screening Libraries:

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View All Bcl-2 Family Isoform Specific Products:

Biological Activity

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References

Customer Review

Humanin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Humanin Related Classifications

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