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HyT36 is a low molecular weight hydrophobic tag that promotes the degradation of fusion protein and pseudokinase Her3.

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HyT36 Chemical Structure

HyT36 Chemical Structure

CAS No. : 1323151-45-6

Size Price Stock Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 165 In-stock
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10 mM * 1 mL in DMSO USD 165 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

HyT36 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HyT36 is a low molecular weight hydrophobic tag that promotes the degradation of fusion protein and pseudokinase Her3[1][2][3].

In Vitro

HyT36 (10 µM; 24 h) decreases the expression of GFP-HaloTag2/Fz4-HaloTag2/GFP-HaloTag7 fusion proteins in HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2[1].
HyT36 (10 µM; 2 h or 48 h) reduces the expression of EGFP and increases the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein in HEK293 Flp-In cells expressing HA-EGFP-HaloTag2[3].
HyT36 (50 nM; 24 h) reduces the level of EGFP-HaloTag2 in HEK293 Flp-In cells expressing HA-EGFP-HaloTag2[3].
HyT36 (1 µM; 4 d) decreases the expression of HA−HaloTag2−HRasG12V in NIH-3T3 cells expressing HA−HaloTag2−HRasG12V[3]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HyT36 Related Antibodies

Western Blot Analysis[1][3]

Cell Line: HEK293 cells expressing GFP-HaloTag2/Fz4-HaloTag2/HA-EGFP-HaloTag2 or NIH-3T3 cells expressing HA−HaloTag2−HRasG12V.
Concentration: 1 µM or 10 µM.
Incubation Time: 2 h, 24 h or 4 days.
Result: Decreased the expression of fusion proteins and increased the binding of Hsp70 and HA-EGFP-HaloTag2 fusion protein.
Molecular Weight

442.07

Formula

C25H44ClNO3
CAS No.
Appearance

Oil

Color

Light yellow to brown

SMILES

ClCCCCCCOCCOCCNC([C@H](C)CCC1(C[C@H]2C3)C[C@H]3C[C@H](C2)C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (226.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2621 mL 11.3104 mL 22.6209 mL
5 mM 0.4524 mL 2.2621 mL 4.5242 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.66 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2621 mL 11.3104 mL 22.6209 mL 56.5521 mL
5 mM 0.4524 mL 2.2621 mL 4.5242 mL 11.3104 mL
10 mM 0.2262 mL 1.1310 mL 2.2621 mL 5.6552 mL
15 mM 0.1508 mL 0.7540 mL 1.5081 mL 3.7701 mL
20 mM 0.1131 mL 0.5655 mL 1.1310 mL 2.8276 mL
25 mM 0.0905 mL 0.4524 mL 0.9048 mL 2.2621 mL
30 mM 0.0754 mL 0.3770 mL 0.7540 mL 1.8851 mL
40 mM 0.0566 mL 0.2828 mL 0.5655 mL 1.4138 mL
50 mM 0.0452 mL 0.2262 mL 0.4524 mL 1.1310 mL
60 mM 0.0377 mL 0.1885 mL 0.3770 mL 0.9425 mL
80 mM 0.0283 mL 0.1414 mL 0.2828 mL 0.7069 mL
100 mM 0.0226 mL 0.1131 mL 0.2262 mL 0.5655 mL
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HyT36 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HyT361323151-45-6HyT 36HyT-36OthersHEK293NIH-3T3GFP-HaloTag2Fz4-HaloTag2GFP-HaloTag7HA-EGFP-HaloTag2HA?HaloTag2?HRasG12VInhibitorinhibitorinhibit

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