I3MT-3 (Synonyms: HMPSNE)

Name: I3MT-3
Brand: MedChemExpress
SKU: HY-128206
Rating: 5.0 (5 reviews)

Cat. No.: HY-128206 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions

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I3MT-3 (HMPSNE) is a potent, selective, and cell-membrane permeable inhibitor of 3-Mercaptopyruvate sulfurtransferase (3MST) (IC₅₀=2.7 μM). I3MT-3 is inactive for other H2S/sulfane sulfur-producing enzymes.?I3MT-3 targets a persulfurated cysteine residue located in the active site of 3MST.

For research use only. We do not sell to patients.

I3MT-3 Chemical Structure

CAS No. : 459420-09-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 231	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 231	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

4 Publications Citing Use of MCE I3MT-3

Proliferation Assay

: I3MT-3 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Oct 30;13(10):913. [Abstract]; Mann-Whitney test. Inhibiting MPST enzymatic activity using the pharmacological inhibitor I3-MT-3 decreases cell migration on Matrigel. Cells treated with 200 µM I3-MT-3 or vehicle for 72 h (R633, P3) or 45 h (GBO). Migration assessed 48 h (R633, P3) and 45 h (GBO) post-seeding.

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Description

IC₅₀ & Target

IC50: 2.7 μM (3-Mercaptopyruvate sulfurtransferase (3MST))^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
CT26	IC₅₀	207 μM Compound: 1a	Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 48 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 48 hrs by IncuCyte live-cell imaging assay	[PMID: 33856792]
CT26	IC₅₀	243 μM Compound: 1a	Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay Antiproliferative activity against mouse CT26 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay	[PMID: 33856792]
MC-38	IC₅₀	101 μM Compound: 1a	Antiproliferative activity against mouse MC38 cells assessed as inhibition of cell proliferation measured after 48 hrs by IncuCyte live-cell imaging assay Antiproliferative activity against mouse MC38 cells assessed as inhibition of cell proliferation measured after 48 hrs by IncuCyte live-cell imaging assay	[PMID: 33856792]
MC-38	IC₅₀	221 μM Compound: 1a	Antiproliferative activity against mouse MC38 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay Antiproliferative activity against mouse MC38 cells assessed as inhibition of cell proliferation measured after 24 hrs by MTT assay	[PMID: 33856792]

In Vitro

I3MT-3 (1 μM) is selective for 3MST and shows a high inhibitory activity (80–90%) even at 10 μM in cell lysate of 3MST-overexpressing HEK293 cells. Besides, it is almost inactive towards the other two H2S-producing enzymes even at 100 μM^[1].
I3MT-3 (1 μM) shows a high selectivity for 3MST, it completely suppresses the 3MST activity in COS7 cells living cells^[1].
I3MT-3 produces a concentration-dependent inhibition of the AzMC (the fluorescent H2S probe) signal when incubated with purified human recombinant enzyme, the inhibition of the catalytic activity of 3-MST produces a concentration-dependent inhibition of H2S production with an IC₅₀ of 13.6 μM^[1].
I3MT-3 shows a dose-dependent inhibition of 3-MST activity from CT26 homogenates, which contain the murine form of the enzyme. The IC₅₀ of HMPSNE for murine 3-MST is calculated as 2.3 μM with a cozncentration-dependent decrease of AzMC fluorescence^[1].
I3MT-3 (10 μM-100 μM; after 3 h probe AzMC) causes a partial inhibition of the signal, while at 100 μM, HMPSNE causes a complete inhibition of the AzMC-guided H2S fluorescence at 100 μM, Additionally, HMPSNE is capable of inhibiting its target in situ in CT26 cells (with an IC₅₀ of approximately 30 μM)^[2].
I3MT-3 (0-300 μM; 5-50 hours) does not enhance MTT conversion at 10 μM, while at 100 and 300 μM it produces an inhibitory response, without increasing the LDH signal, i.e., without inducing any detectable degree of cell necrosis. It also produces a decreased oxygen consumption rate (OCR) profiles in CT26 cells^[2].
I3MT-3 (0-300 μM; 48 hours) inhibits CT26 cells proliferate with increasing concentrations of I3MT-3. Confluence of cells treated with HMPSNE is recorded each hour for 48 h by the IncuCyte method^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	CT26 cells
Concentration:	0 μM; 10 μM; 30 μM; 100 μM; 300 μM
Incubation Time:	48 hours
Result:	Slowed down proliferation of CT26 cells.

Molecular Weight

310.37

Formula

C₁₇H₁₄N₂O₂S

CAS No.

459420-09-8

Appearance

Solid

Color

Off-white to light brown

SMILES

O=C1NC(SCC(C2=C3C=CC=CC3=CC=C2)=O)=NC(C)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (402.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2220 mL	16.1098 mL	32.2196 mL
5 mM	0.6444 mL	3.2220 mL	6.4439 mL
10 mM	0.3222 mL	1.6110 mL	3.2220 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (6.70 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (252 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Kenjiro Hanaoka, et al. Discovery and Mechanistic Characterization of Selective Inhibitors of H 2 S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide. Sci Rep. 2017 Jan 12;7:40227 [Content Brief]

[2]. Fiona Augsburger, et al. Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics in Murine Colon Cancer Cells. Biomolecules. 2020 Mar 13;10(3):447. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2220 mL	16.1098 mL	32.2196 mL	80.5490 mL
	5 mM	0.6444 mL	3.2220 mL	6.4439 mL	16.1098 mL
	10 mM	0.3222 mL	1.6110 mL	3.2220 mL	8.0549 mL
	15 mM	0.2148 mL	1.0740 mL	2.1480 mL	5.3699 mL
	20 mM	0.1611 mL	0.8055 mL	1.6110 mL	4.0275 mL
	25 mM	0.1289 mL	0.6444 mL	1.2888 mL	3.2220 mL
	30 mM	0.1074 mL	0.5370 mL	1.0740 mL	2.6850 mL
	40 mM	0.0805 mL	0.4027 mL	0.8055 mL	2.0137 mL
	50 mM	0.0644 mL	0.3222 mL	0.6444 mL	1.6110 mL
	60 mM	0.0537 mL	0.2685 mL	0.5370 mL	1.3425 mL
	80 mM	0.0403 mL	0.2014 mL	0.4027 mL	1.0069 mL
	100 mM	0.0322 mL	0.1611 mL	0.3222 mL	0.8055 mL

I3MT-3 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

I3MT-3459420-09-8HMPSNEHippo (MST)HEK2933MSTECEA.hy926 cellsCOS-7cell-membraneInhibitorinhibitorinhibit

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I3MT-3 (Synonyms: HMPSNE)

Customer Review

I3MT-3 Related Antibodies

4 Publications Citing Use of MCE I3MT-3

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Complete Stock Solution Preparation Table

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