IM-54

Name: IM-54
Brand: MedChemExpress
SKU: HY-108351
Rating: 4.5 (1 reviews)

Cat. No.: HY-108351 Purity: 99.06%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

IM-54 is a selective inhibitor of oxidative stress-induced necrosis. IM-54 shows potent inhibitory activity against H₂O₂-induced necrosis. IM-54 acts as a potential cardioprotective agent and biological tool for investigating the molecular mechanisms of cell death.

For research use only. We do not sell to patients.

IM-54 Chemical Structure

CAS No. : 861891-50-1

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 204	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 367	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 514	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 720	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
H9c2	IC₅₀	3.9 μM Compound: IM-54	Cytoprotective activity against TBHP-induced necrosis in rat H9c2 cells incubated for 4 hrs by LDH release assay Cytoprotective activity against TBHP-induced necrosis in rat H9c2 cells incubated for 4 hrs by LDH release assay	[PMID: 29541357]
HL-60	IC₅₀	0.25 μM Compound: IM-54	Cytoprotective activity against H2O2-induced necrosis in human HL60 cells incubated for 1 hr followed by H2O2 stimulation by LDH release assay Cytoprotective activity against H2O2-induced necrosis in human HL60 cells incubated for 1 hr followed by H2O2 stimulation by LDH release assay	[PMID: 29541357]

In Vitro

IM-54 selectively inhibits oxidative stress-induced necrosis, but it does not inhibit apoptosis induced by various anticancer drugs or Fas ligand, or necroptosis^[1].
IM-54 shows strong inhibition of H₂O₂-induced necrosis, with an IC₅₀ of 0.25 μM in HL-60 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

325.40

Formula

C₁₉H₂₃N₃O₂

CAS No.

861891-50-1

Appearance

Solid

Color

Light yellow to orange

SMILES

O=C(C(C1=CN(C)C2=C1C=CC=C2)=C3NCCCCC)N(C)C3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (153.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0731 mL	15.3657 mL	30.7314 mL
5 mM	0.6146 mL	3.0731 mL	6.1463 mL
10 mM	0.3073 mL	1.5366 mL	3.0731 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.31%

Select Batch:

Data Sheet (276 KB) SDS (420 KB)

COA (254 KB) RP-HPLC (208 KB) LCMS (254 KB)

Handling Instructions (2659 KB)

References

[1]. Kosuke Dodo, et al. Indolylmaleimide Derivative IM-17 Shows Cardioprotective Effects in Ischemia-Reperfusion Injury. ACS Med Chem Lett. 2018 Mar 8; 9(3): 182-187. [Content Brief]

[2]. Mikiko Sodeoka, et al. Development of selective inhibitors of necrosis. Chem Rec. 2010 Oct;10(5):308-14. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0731 mL	15.3657 mL	30.7314 mL	76.8285 mL
	5 mM	0.6146 mL	3.0731 mL	6.1463 mL	15.3657 mL
	10 mM	0.3073 mL	1.5366 mL	3.0731 mL	7.6829 mL
	15 mM	0.2049 mL	1.0244 mL	2.0488 mL	5.1219 mL
	20 mM	0.1537 mL	0.7683 mL	1.5366 mL	3.8414 mL
	25 mM	0.1229 mL	0.6146 mL	1.2293 mL	3.0731 mL
	30 mM	0.1024 mL	0.5122 mL	1.0244 mL	2.5610 mL
	40 mM	0.0768 mL	0.3841 mL	0.7683 mL	1.9207 mL
	50 mM	0.0615 mL	0.3073 mL	0.6146 mL	1.5366 mL
	60 mM	0.0512 mL	0.2561 mL	0.5122 mL	1.2805 mL
	80 mM	0.0384 mL	0.1921 mL	0.3841 mL	0.9604 mL
	100 mM	0.0307 mL	0.1537 mL	0.3073 mL	0.7683 mL

IM-54 Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IM-54861891-50-1IM54IM 54Necroptosisoxidativestressnecrosisischemia-reperfusionI/RinjuriesarrhythmiacardioprotectiveInhibitorinhibitorinhibit

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IM-54

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Complete Stock Solution Preparation Table

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