Justicidin B

Name: Justicidin B
Brand: MedChemExpress
SKU: HY-114275
Rating: 4.5 (1 reviews)

Cat. No.: HY-114275 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Justicidin B is a potent anticancer lignan and proapoptotic agent. Justicidin B is also a bone resorption inhibitor, and has strong antiviral, fungicidal, antiprotozoal effects. Justicidin B significantly inhibits platelet aggregation.

For research use only. We do not sell to patients.

Justicidin B Chemical Structure

CAS No. : 17951-19-8

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Trypanosoma

Cellular Effect

Cell Line	Type	Value	Description	References
A2058	IC₅₀	8.9 μM Compound: 2	Cytotoxicity against human A2058 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human A2058 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
A549	IC₅₀	11.4 μM Compound: 2	Cytotoxicity against human A549 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human A549 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
BGC-823	IC₅₀	0.18 μM Compound: 2	Cytotoxicity against human BGC-823 cells assessed as reduction in cell viability measured after 4 days by MTT colorimetric assay Cytotoxicity against human BGC-823 cells assessed as reduction in cell viability measured after 4 days by MTT colorimetric assay	[PMID: 36857518]
Calu-1	IC₅₀	18.5 μM Compound: 2	Cytotoxicity against human Calu-1 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human Calu-1 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
EBC-1	IC₅₀	63.2 μM Compound: 2	Cytotoxicity against human EBC-1 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human EBC-1 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
HepG2	IC₅₀	16.8 μM Compound: 2	Cytotoxicity against human HepG2 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human HepG2 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
HT-29	IC₅₀	5.9 μM Compound: 2	Cytotoxicity against human HT-29 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human HT-29 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
MONO-MAC-6	IC₅₀	2.5 μM Compound: 2	Cytotoxicity against human MONO-MAC-6 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human MONO-MAC-6 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
NCI-H838	IC₅₀	11 μM Compound: 2	Cytotoxicity against human NCI-H838 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human NCI-H838 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
PC-3	IC₅₀	5.7 μM Compound: 2	Cytotoxicity against human PC-3 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human PC-3 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]
RPMI-8226	IC₅₀	0.17 μM Compound: 2	Cytotoxicity against human RPMI-8226 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against human RPMI-8226 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 36857518]
U-87MG ATCC	IC₅₀	21.6 μM Compound: 2	Cytotoxicity against human U87 cells incubated for 24 hrs by AlamarBlue staining based analysis Cytotoxicity against human U87 cells incubated for 24 hrs by AlamarBlue staining based analysis	[PMID: 36857518]

In Vitro

Justicidin B inhibits the growth of the pathogenic fungi Aspergillus fumigatus (MIC ≥ 1 μg/mL), Aspergillus flavus (MIC ≥ 12 μg/mL), and Candida albicans (MIC ≥ 4 μg/mL), but is not effective against Cryptococcus neoformans and Blastoschizomyces capitatus^[1].
Justicidin B also exhibits strong activity against the trypomastigote form of Trypanosoma brucei rhodesiense (IC₅₀ = 0.2 μg/mL) and moderate activity against Trypanosoma cruzi (IC₅₀0 = 2.6 μg/mL)^[1].
Justicidin B shows cytotoxic activity and induction of apoptosis in MDA-MB-231 and MCF-7 breast cancer derived cell lines. The 24 h treatment of both cell lines increased the level of apoptotic DNA fragmentation. Exposure of MDA-MB-231 cells with Justicidin B leads to concentration dependent decrease in the expression of NFkB; whereas the treatment of MCF-7, is consistent with strong increase in the expression of this transcription factor^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

364.35

Formula

C₂₁H₁₆O₆

CAS No.

17951-19-8

Appearance

Solid

Color

White to off-white

SMILES

O=C1OCC2=CC3=CC(OC)=C(OC)C=C3C(C4=CC=C(OCO5)C5=C4)=C12

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (34.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7446 mL	13.7231 mL	27.4461 mL
5 mM	0.5489 mL	2.7446 mL	5.4892 mL
10 mM	0.2745 mL	1.3723 mL	2.7446 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (3.43 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (3.43 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (282 KB) SDS (251 KB)

COA (260 KB)

Handling Instructions (2659 KB)

References

[1]. Jürg Gertsch, et al. Antifungal, antiprotozoal, cytotoxic and piscicidal properties of Justicidin B and a new arylnaphthalide lignan from Phyllanthus piscatorum. Planta Med. 2003 May;69(5):420-4. [Content Brief]

[2]. G Momekov, et al. Effect of justicidin B - a potent cytotoxic and pro-apoptotic arylnaphtalene lignan on human breast cancer-derived cell lines. Neoplasma. 2011;58(4):320-5. [Content Brief]

[3]. Iliana Ionkova, et al. Linum narbonense: A new valuable tool for biotechnological production of a potent anticancer lignan Justicidine B. Pharmacogn Mag. 2013 Jan;9(33):39-44. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7446 mL	13.7231 mL	27.4461 mL	68.6153 mL
	5 mM	0.5489 mL	2.7446 mL	5.4892 mL	13.7231 mL
	10 mM	0.2745 mL	1.3723 mL	2.7446 mL	6.8615 mL
	15 mM	0.1830 mL	0.9149 mL	1.8297 mL	4.5744 mL
	20 mM	0.1372 mL	0.6862 mL	1.3723 mL	3.4308 mL
	25 mM	0.1098 mL	0.5489 mL	1.0978 mL	2.7446 mL
	30 mM	0.0915 mL	0.4574 mL	0.9149 mL	2.2872 mL

Justicidin B Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Justicidin B17951-19-8FungalParasiteApoptosisantirheumaticbone resorption inhibitorantiviralfungicidalantiprotozoal effectsInhibitorinhibitorinhibit

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Justicidin B

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Complete Stock Solution Preparation Table

Justicidin B Related Classifications

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