2. Cell Cycle/DNA Damage Cytoskeleton
  3. Microtubule/Tubulin
  4. KIF18A-IN-6

KIF18A-IN-6 (Compound 134) is an orally active KIF18A inhibitor with an IC50 of 0.016 μM against KIF18A microtubule-dependent ATPase activity.

For research use only. We do not sell to patients.

KIF18A-IN-6 Chemical Structure

KIF18A-IN-6 Chemical Structure

CAS No. : 2914879-10-8

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5 mg USD 380 In-stock
10 mg USD 620 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

KIF18A-IN-6 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

KIF18A-IN-6 (Compound 134) is an orally active KIF18A inhibitor with an IC50 of 0.016 μM against KIF18A microtubule-dependent ATPase activity[1].

IC50 & Target

IC50: 0.016 μM (KIF18A microtubule-dependent ATPase activity)[1]
In Vitro

KIF18A-IN-6 (Compound 134; 7 days) inhibits JIMT-1, HCC-15 and NIH-OVCAR3 cells viability with IC50s of 0.0040, 0.0051 and 0.0051 μM, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KIF18A-IN-6 Related Antibodies

Cell Viability Assay[1]

Cell Line: HCC-15, JIMT-1 and NIH-OVCAR3
Concentration:
Incubation Time: 7 days
Result: Inhibited cell viability with IC50s of 0.0040, 0.0051 and 0.0051 μM against JIMT-1, HCC-15 and NIH-OVCAR3 cells, respectively.
In Vivo

KIF18A-IN-6 (Compound 134; 10-60 mg/kg; p.o.; twice or once a day for 1 month) inhibits HCC15 and OVCAR3 tumor growth in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: SCID Beige mice, HCC15 tumor model[1]
Dosage: 10, 30 and 60 mg/kg
Administration: PO, twice a day for 1 month
Result: Inhibited tumor growth by 61±10%, 89±7% and 94±5% at 10, 30 and 60 mg/kg, respectively.
Animal Model: Balb/C nude mice, OVCAR3 tumor model[1]
Dosage: 10, 30 and 60 mg/kg
Administration: PO, twice or once a day for 1 month
Result: Completely inhibited tumor growth (over 100%) over 30 mg/kg.
Molecular Weight

559.74

Formula

C28H37N3O5S2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC(C(N2CC3(CCC4(CC4)CC3)C5=CC(NS(CC)(=O)=O)=CC=C52)=O)=CC=C1)(NC(C)(C)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (223.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7865 mL 8.9327 mL 17.8654 mL
5 mM 0.3573 mL 1.7865 mL 3.5731 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (3.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.56%

SDS (251 KB)

COA (255 KB) HNMR (294 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7865 mL 8.9327 mL 17.8654 mL 44.6636 mL
5 mM 0.3573 mL 1.7865 mL 3.5731 mL 8.9327 mL
10 mM 0.1787 mL 0.8933 mL 1.7865 mL 4.4664 mL
15 mM 0.1191 mL 0.5955 mL 1.1910 mL 2.9776 mL
20 mM 0.0893 mL 0.4466 mL 0.8933 mL 2.2332 mL
25 mM 0.0715 mL 0.3573 mL 0.7146 mL 1.7865 mL
30 mM 0.0596 mL 0.2978 mL 0.5955 mL 1.4888 mL
40 mM 0.0447 mL 0.2233 mL 0.4466 mL 1.1166 mL
50 mM 0.0357 mL 0.1787 mL 0.3573 mL 0.8933 mL
60 mM 0.0298 mL 0.1489 mL 0.2978 mL 0.7444 mL
80 mM 0.0223 mL 0.1117 mL 0.2233 mL 0.5583 mL
100 mM 0.0179 mL 0.0893 mL 0.1787 mL 0.4466 mL
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KIF18A-IN-6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KIF18A-IN-62914879-10-8Microtubule/TubulinHCC-15JIMT-1NIH-OVCAR3SCID Beige miceBalb/C nude miceInhibitorinhibitorinhibit

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