L-NMMA citrate (Synonyms: Methylarginine citrate; Tilarginine citrate)

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L-NMMA citrate competitively antagonizes L-arginine, and thus inhibits production of nitric oxide (NO). L-NMMA is utilized as NO synthase antagonist in experimental settings.

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L-NMMA citrate Chemical Structure

CAS No. : 209913-88-2

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Other In-stock Forms of L-NMMA citrate:

L-NMMA acetate

Other Forms of L-NMMA citrate:

L-NMMA acetate In-stock
L-NMMA Get quote
L-NMMA hydrochloride Get quote

8 Publications Citing Use of MCE L-NMMA citrate

Cell Viability Assay

: L-NMMA citrate purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]; L-NMMA acetate (200 μM; 48 h) leads to elevating ammonia and inhibiting CD8⁺ T_M cell induction, in parallel with the inhibition of NO production.

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nNOS iNOS eNOS

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Description

L-NMMA citrate competitively antagonizes L-arginine, and thus inhibits production of nitric oxide (NO). L-NMMA is utilized as NO synthase antagonist in experimental settings^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	22.7 μM Compound: L-NMMA	Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS stimulation and measured after 20 hrs by Griess reagent based assay Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS stimulation and measured after 20 hrs by Griess reagent based assay	[PMID: 36746775]
RAW264.7	IC₅₀	25.1 μM Compound: L-NMMA	Cancer chemopreventive activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent assay Cancer chemopreventive activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent assay	[PMID: 23316950]
RAW264.7	IC₅₀	25.1 μM Compound: N-monomethyl-L-arginine citrate	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide overproduction pretreated with LPS for 24 hrs followed by overnight incubation with compound by Griess assay Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide overproduction pretreated with LPS for 24 hrs followed by overnight incubation with compound by Griess assay	[PMID: 31411887]
RAW264.7	IC₅₀	25.1 μM Compound: L-NMMA	Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level preincubated for 15 mins prior to LPS treatment by Griess method Antiinflammatory activity against LPS-induced NO production in mouse RAW264.7 cells assessed as reduction in nitrite level preincubated for 15 mins prior to LPS treatment by Griess method	[PMID: 22850207]
RAW264.7	IC₅₀	25.1 μM Compound: L-NMMA	Cytotoxicity against mouse RAW264.7 cells assessed as cell survival by SRB assay Cytotoxicity against mouse RAW264.7 cells assessed as cell survival by SRB assay	[PMID: 23316950]
RAW264.7	IC₅₀	32 μM Compound: L-NMMA	Inhibition of iNOS-mediated NO production in mouse RAW264.7 cells by Griess assay Inhibition of iNOS-mediated NO production in mouse RAW264.7 cells by Griess assay	[PMID: 23994869]

Molecular Weight

380.35

Formula

C₁₃H₂₄N₄O₉

CAS No.

209913-88-2

SMILES

N[C@H](C(O)=O)CCCNC(NC)=N.O=C(O)CC(O)(CC(O)=O)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Frey C, et al., L-thiocitrulline. A stereospecific, heme-binding inhibitor of nitric-oxide synthases. J Biol Chem. 1994 Oct 21;269(42):26083-91. [Content Brief]

L-NMMA citrate Related Classifications

Molarity Calculator
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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-NMMA209913-88-2MethylarginineTilarginineNO SynthaseEndogenous MetaboliteNitric oxide synthasesNOSCompetitiveNONO synthaseInhibitorinhibitorinhibit

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