1. Immunology/Inflammation
  2. NO Synthase
  3. L-NMMA

L-NMMA  (Synonyms: Tilarginine; Methylarginine)

Cat. No.: HY-18732
Handling Instructions

L-NMMA (Tilarginine; Methylarginine) competitively antagonizes L-arginine, and thus inhibits production of nitric oxide (NO). L-NMMA is utilized as NO synthase antagonist in experimental settings.

For research use only. We do not sell to patients.

L-NMMA Chemical Structure

L-NMMA Chemical Structure

CAS No. : 17035-90-4

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Other In-stock Forms of L-NMMA:

Other Forms of L-NMMA:

Top Publications Citing Use of Products

    L-NMMA purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173.  [Abstract]

    L-NMMA acetate (200 μM; 48 h) leads to elevating ammonia and inhibiting CD8+ TM cell induction, in parallel with the inhibition of NO production.

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    • Biological Activity

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    Description

    L-NMMA (Tilarginine; Methylarginine) competitively antagonizes L-arginine, and thus inhibits production of nitric oxide (NO). L-NMMA is utilized as NO synthase antagonist in experimental settings[1].

    Cellular Effect
    Cell Line Type Value Description References
    BV-2 CC50
    > 200 μM
    Compound: L-NMMA
    Cytotoxicity against mouse BV-2 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    Cytotoxicity against mouse BV-2 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay
    [PMID: 34161733]
    BV-2 IC50
    10 μM
    Compound: L-NMMA
    Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production measured after 24 hrs by Griess reagent based assay
    Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production measured after 24 hrs by Griess reagent based assay
    [PMID: 34161733]
    BV-2 IC50
    13.2 μM
    Compound: L-NMMA
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 30 mins followed by LPS-stimulation after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 30 mins followed by LPS-stimulation after 24 hrs by Griess assay
    [PMID: 28841320]
    BV-2 IC50
    14.29 μM
    Compound: L-NMMA
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent method
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent method
    [PMID: 23411079]
    BV-2 IC50
    14.4 μM
    Compound: L-NMMA
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 4 hrs followed by LPS stimulation for 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 4 hrs followed by LPS stimulation for 24 hrs by Griess assay
    [PMID: 27140807]
    BV-2 IC50
    14.4 μM
    Compound: L-NMMA
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production preincubated for 4 hrs followed by LPS challenge measured after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production preincubated for 4 hrs followed by LPS challenge measured after 24 hrs by Griess assay
    [PMID: 26126961]
    BV-2 IC50
    15.41 μM
    Compound: L-NMMA
    Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS-stimulation measured after 24 hrs by Griess reaction
    Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS-stimulation measured after 24 hrs by Griess reaction
    [PMID: 21936523]
    BV-2 IC50
    16.05 μM
    Compound: N-NMMA
    Anti-inflammatory activity in mouse BV2 cells assessed as reduction in LPS-induced NO production pre-treated before LPS stimulation for 24 hrs by Griess reagent-based assay
    Anti-inflammatory activity in mouse BV2 cells assessed as reduction in LPS-induced NO production pre-treated before LPS stimulation for 24 hrs by Griess reagent-based assay
    [PMID: 30530196]
    BV-2 IC50
    16.27 μM
    Compound: NMMA
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    [PMID: 26774654]
    BV-2 IC50
    16.27 μM
    Compound: NMMA
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
    [PMID: 23149227]
    BV-2 IC50
    16.32 μM
    Compound: L-NMMA
    Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control
    Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control
    [PMID: 23462643]
    BV-2 IC50
    16.8 μM
    Compound: L-NMMA
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method
    [PMID: 19900811]
    BV-2 IC50
    16.8 μM
    Compound: L-NMMA
    Antiinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 30 mins prior to LPS-challenge measured after 24 hrs by Griess assay
    Antiinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 30 mins prior to LPS-challenge measured after 24 hrs by Griess assay
    [PMID: 23815260]
    BV-2 IC50
    17 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
    [PMID: 31082231]
    BV-2 IC50
    17.6 μM
    Compound: NG-monomethyl-L-arginine
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    [PMID: 27700070]
    BV-2 IC50
    18.25 μM
    Compound: NMMA
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction
    [PMID: 22951040]
    BV-2 IC50
    18.26 μM
    Compound: NMMA
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
    [PMID: 23707257]
    BV-2 IC50
    18.3 μM
    Compound: NMMA
    Inhibition of LPS-induced nitric oxide production in mouse BV2 cells treated for 30 mins prior to LPS stimulation for 24 hrs by Griess assay
    Inhibition of LPS-induced nitric oxide production in mouse BV2 cells treated for 30 mins prior to LPS stimulation for 24 hrs by Griess assay
    [PMID: 25466198]
    BV-2 IC50
    20.53 μM
    Compound: L-NMMA
    Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    [PMID: 26706168]
    BV-2 IC50
    20.78 μM
    Compound: L-NMMA
    Anti-neuroinflammatory activity in LPS-activated mouse BV2 cells assessed as inhibition of NO production after 24 hrs by Griess assay
    Anti-neuroinflammatory activity in LPS-activated mouse BV2 cells assessed as inhibition of NO production after 24 hrs by Griess assay
    [PMID: 26988298]
    BV-2 IC50
    21.25 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay
    [PMID: 28165740]
    BV-2 IC50
    21.37 μM
    Compound: L-NMMA
    Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production incubated for 48 hrs by Griess reagent based assay
    Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production incubated for 48 hrs by Griess reagent based assay
    [PMID: 34546050]
    BV-2 IC50
    21.4 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay
    [PMID: 30063340]
    BV-2 IC50
    21.4 μM
    Compound: L-NMMA
    Cytotoxicity against mouse BV2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    Cytotoxicity against mouse BV2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
    [PMID: 30232882]
    BV-2 IC50
    21.8 μM
    Compound: L-NMMA
    Anti-inflammatory in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pre-incubated before LPS challenge for 24 hrs by Griess reagent based assay
    Anti-inflammatory in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pre-incubated before LPS challenge for 24 hrs by Griess reagent based assay
    [PMID: 28358502]
    BV-2 IC50
    21.9 μM
    Compound: L-NMMA
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition in LPS-induced NO production preincubated for 30 mins followed b LPS addition and measured after 24 hr by griess reagent based assay
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition in LPS-induced NO production preincubated for 30 mins followed b LPS addition and measured after 24 hr by griess reagent based assay
    [PMID: 31625742]
    BV-2 IC50
    22.1 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production by Griess assay
    [PMID: 28262525]
    BV-2 IC50
    24.3 μM
    Compound: L-NMMA
    Antineuroinflammatory activity against human BV2 cells assessed as inhibition of LPS-induced NO production treated with compound 30 mins and challenged with LPS for 24 hrs
    Antineuroinflammatory activity against human BV2 cells assessed as inhibition of LPS-induced NO production treated with compound 30 mins and challenged with LPS for 24 hrs
    [PMID: 25098650]
    BV-2 IC50
    24.8 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    [PMID: 26812172]
    BV-2 IC50
    24.8 μM
    Compound: L-NMMA
    Antiinflammatory activity against LPS-stimulated mouse BV2 cells assessed as inhibition of nitric oxide production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess assay
    Antiinflammatory activity against LPS-stimulated mouse BV2 cells assessed as inhibition of nitric oxide production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess assay
    [PMID: 25894905]
    BV-2 IC50
    26.58 μM
    Compound: L-NMMA
    Anti-inflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay
    Anti-inflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay
    [PMID: 30472026]
    BV-2 IC50
    28.8 μM
    Compound: L-NMMA
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess assay
    Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess assay
    [PMID: 32520551]
    BV-2 IC50
    31.8 μM
    Compound: L-NMMA
    Antineuroinflammatory activity against mouse BV-2 cells assessed as inhibition of LPS-induced NO production by Griess assay
    Antineuroinflammatory activity against mouse BV-2 cells assessed as inhibition of LPS-induced NO production by Griess assay
    [PMID: 34964626]
    BV-2 IC50
    39.28 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in human BV2 cells preincubated for 2 hrs followed by LPS-stimulation measured after 18 hrs by Griess assay
    Inhibition of LPS-induced NO production in human BV2 cells preincubated for 2 hrs followed by LPS-stimulation measured after 18 hrs by Griess assay
    [PMID: 27525351]
    DLD-1 IC50
    0.3 μM
    Compound: 8 (L-NMMA)
    Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by i-NOS from human DLD-1 cells
    Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by i-NOS from human DLD-1 cells
    [PMID: 11327581]
    DLD-1 IC50
    0.3 μM
    Compound: 1
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells
    [PMID: 11327580]
    HUVEC IC50
    0.3 μM
    Compound: 8 (L-NMMA)
    Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by e-NOS from HUVEC cells
    Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by e-NOS from HUVEC cells
    [PMID: 11327581]
    HUVEC IC50
    0.3 μM
    Compound: 1
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells
    [PMID: 11327580]
    J774.1 IC50
    13.8 μg/mL
    Compound: L-NMMA
    Inhibition of LPS-induced nitric oxide production in mouse J774.1 cells after 24 hrs by griess assay
    Inhibition of LPS-induced nitric oxide production in mouse J774.1 cells after 24 hrs by griess assay
    [PMID: 21428417]
    J774.1 IC50
    26 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs
    Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs
    [PMID: 12608860]
    J774.1 IC50
    27.1 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay
    Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay
    [PMID: 15974608]
    J774.1 IC50
    27.1 μM
    Compound: L-NMMA
    Cytotoxicity against LPS induced mouse J774.1 cells by MTT assay
    Cytotoxicity against LPS induced mouse J774.1 cells by MTT assay
    [PMID: 18154272]
    J774.1 IC50
    35.7 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in mouse J774.1cells after 24 hrs
    Inhibition of LPS-induced NO production in mouse J774.1cells after 24 hrs
    [PMID: 15104497]
    Macrophage IC50
    28 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method
    Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method
    [PMID: 12398545]
    Macrophage IC50
    57 μM
    Compound: L-NMMA
    Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay
    Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay
    [PMID: 15270564]
    RAW IC50
    19.7 μM
    Compound: L-NMMA
    Inhibition of LPS-induced iNOS activity in mouse RAW 264.7 cells assessed as inhibition of NO production pretreated with compound for 30 mins before LPS challenge after 24 hrs by Griess reagent method
    Inhibition of LPS-induced iNOS activity in mouse RAW 264.7 cells assessed as inhibition of NO production pretreated with compound for 30 mins before LPS challenge after 24 hrs by Griess reagent method
    [PMID: 23142320]
    RAW IC50
    22.1 μM
    Compound: L-NG-monomethyl arginine
    Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW 264.7 cells pretreated 15 mins before LPS challenge measured after 20 hrs relative to control
    Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW 264.7 cells pretreated 15 mins before LPS challenge measured after 20 hrs relative to control
    [PMID: 21978950]
    RAW IC50
    25 μM
    Compound: 2
    Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells
    Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells
    [PMID: 9651169]
    RAW264.7 IC50
    > 40.5 μM
    Compound: N-monomethyl-L-arginine
    Inhibition of LPS-induced NO release in mouse RAW264.7 cells after 24 hrs by Griess reaction
    Inhibition of LPS-induced NO release in mouse RAW264.7 cells after 24 hrs by Griess reaction
    [PMID: 26052978]
    RAW264.7 IC50
    > 50 μM
    Compound: L-NMMA
    Cytotoxicity against mouse RAW264.7 cells by MTT assay
    Cytotoxicity against mouse RAW264.7 cells by MTT assay
    [PMID: 16643064]
    RAW264.7 IC50
    16.11 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 18 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 18 hrs by Griess assay
    [PMID: 29685654]
    RAW264.7 IC50
    19.2 μM
    Compound: L-NMMA
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs
    [PMID: 16643064]
    RAW264.7 IC50
    19.7 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins measured 24 hrs post LPS challenge by Griess method
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins measured 24 hrs post LPS challenge by Griess method
    [PMID: 22386564]
    RAW264.7 IC50
    19.7 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins before LPS challenge measured after 24 hrs by Griess method
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins before LPS challenge measured after 24 hrs by Griess method
    [PMID: 22115839]
    RAW264.7 IC50
    19.9 μM
    Compound: 1, L-NMMA
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 20 hrs by Griess reaction
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 20 hrs by Griess reaction
    [PMID: 21377876]
    RAW264.7 IC50
    2.3 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS activity incubated for 30 mins prior to LPS challenge measured after 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS activity incubated for 30 mins prior to LPS challenge measured after 24 hrs by Griess assay
    [PMID: 25975638]
    RAW264.7 IC50
    2.69 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay
    [PMID: 26898337]
    RAW264.7 IC50
    2.73 μM
    Compound: L-NMMA
    Cytotoxicity against mouse RAW264.7 cells assessed as decrease in cell proliferation rate after 24 hrs by MTS assay
    Cytotoxicity against mouse RAW264.7 cells assessed as decrease in cell proliferation rate after 24 hrs by MTS assay
    [PMID: 27777005]
    RAW264.7 IC50
    21.72 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production incubated for 15 mins prior to LPS challenge measured after 20 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production incubated for 15 mins prior to LPS challenge measured after 20 hrs by Griess assay
    [PMID: 24016057]
    RAW264.7 IC50
    23.5 μM
    Compound: L-NMMA
    Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay
    Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay
    [PMID: 27210437]
    RAW264.7 IC50
    25.1 μM
    Compound: L-NMMA
    Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS challenge measured after 20 hrs by Griess assay
    Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS challenge measured after 20 hrs by Griess assay
    [PMID: 26343828]
    RAW264.7 IC50
    25.8 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay
    [PMID: 29400471]
    RAW264.7 IC50
    27.2 μM
    Compound: L-NMMA
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs
    [PMID: 31710213]
    RAW264.7 IC50
    30.92 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production pretreated for 2 hrs followed by LPS-stimulation measured after 20 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production pretreated for 2 hrs followed by LPS-stimulation measured after 20 hrs by Griess assay
    [PMID: 28753309]
    RAW264.7 IC50
    32.9 μM
    Compound: L-NMMA
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess assay
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess assay
    [PMID: 30296083]
    RAW264.7 IC50
    35.5 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
    [PMID: 33939429]
    RAW264.7 IC50
    39.2 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 2 hrs prior to LPS-challenge measured after 24 hrs by Greiss assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 2 hrs prior to LPS-challenge measured after 24 hrs by Greiss assay
    [PMID: 25924023]
    RAW264.7 IC50
    40.4 μM
    Compound: N-monomethyl-L-arginine
    Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells by Griess reagent assay
    Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells by Griess reagent assay
    [PMID: 23394284]
    RAW264.7 IC50
    44.36 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production incubated for 1 hrs followed by LPS stimulation for 18 hrs by Griess reagent based assay
    Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production incubated for 1 hrs followed by LPS stimulation for 18 hrs by Griess reagent based assay
    [PMID: 35427124]
    RAW264.7 IC50
    45 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay
    [PMID: 26741483]
    RAW264.7 IC50
    73.3 μM
    Compound: L-NMMA
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production compound pretreated for 1 hr followed by addition of 100 ng/ml LPS for 24 hrs by griess method
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production compound pretreated for 1 hr followed by addition of 100 ng/ml LPS for 24 hrs by griess method
    [PMID: 26681510]
    Sf9 IC50
    0.65 μM
    Compound: L-NMMA
    Inhibition of human eNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method
    Inhibition of human eNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method
    [PMID: 22335555]
    Sf9 IC50
    0.95 μM
    Compound: L-NMMA
    Inhibition of human nNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method
    Inhibition of human nNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method
    [PMID: 22335555]
    Sf9 IC50
    1.8 μM
    Compound: L-NMMA
    Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method
    Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method
    [PMID: 22335555]
    Molecular Weight

    188.23

    Formula

    C7H16N4O2

    CAS No.
    SMILES

    OC([C@@H](N)CCCNC(NC)=N)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.

    Purity & Documentation
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    L-NMMA Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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