L-NMMA (Synonyms: Tilarginine; Methylarginine)

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L-NMMA (Tilarginine; Methylarginine) competitively antagonizes L-arginine, and thus inhibits production of nitric oxide (NO). L-NMMA is utilized as NO synthase antagonist in experimental settings.

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L-NMMA Chemical Structure

CAS No. : 17035-90-4

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Other In-stock Forms of L-NMMA:

L-NMMA acetate

Other Forms of L-NMMA:

L-NMMA acetate In-stock
L-NMMA citrate Get quote
L-NMMA hydrochloride Get quote

8 Publications Citing Use of MCE L-NMMA

Cell Viability Assay

: L-NMMA purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2023 Jan;24(1):162-173. [Abstract]; L-NMMA acetate (200 μM; 48 h) leads to elevating ammonia and inhibiting CD8⁺ T_M cell induction, in parallel with the inhibition of NO production.

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Biological Activity
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Description

L-NMMA (Tilarginine; Methylarginine) competitively antagonizes L-arginine, and thus inhibits production of nitric oxide (NO). L-NMMA is utilized as NO synthase antagonist in experimental settings^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
BV-2	CC₅₀	> 200 μM Compound: L-NMMA	Cytotoxicity against mouse BV-2 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against mouse BV-2 cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 34161733]
BV-2	IC₅₀	10 μM Compound: L-NMMA	Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production measured after 24 hrs by Griess reagent based assay Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production measured after 24 hrs by Griess reagent based assay	[PMID: 34161733]
BV-2	IC₅₀	13.2 μM Compound: L-NMMA	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 30 mins followed by LPS-stimulation after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 30 mins followed by LPS-stimulation after 24 hrs by Griess assay	[PMID: 28841320]
BV-2	IC₅₀	14.29 μM Compound: L-NMMA	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent method Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of nitric oxide production treated 30 mins before LPS challenge measured after 24 hrs by Griess reagent method	[PMID: 23411079]
BV-2	IC₅₀	14.4 μM Compound: L-NMMA	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 4 hrs followed by LPS stimulation for 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 4 hrs followed by LPS stimulation for 24 hrs by Griess assay	[PMID: 27140807]
BV-2	IC₅₀	14.4 μM Compound: L-NMMA	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production preincubated for 4 hrs followed by LPS challenge measured after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production preincubated for 4 hrs followed by LPS challenge measured after 24 hrs by Griess assay	[PMID: 26126961]
BV-2	IC₅₀	15.41 μM Compound: L-NMMA	Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS-stimulation measured after 24 hrs by Griess reaction Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins before LPS-stimulation measured after 24 hrs by Griess reaction	[PMID: 21936523]
BV-2	IC₅₀	16.05 μM Compound: N-NMMA	Anti-inflammatory activity in mouse BV2 cells assessed as reduction in LPS-induced NO production pre-treated before LPS stimulation for 24 hrs by Griess reagent-based assay Anti-inflammatory activity in mouse BV2 cells assessed as reduction in LPS-induced NO production pre-treated before LPS stimulation for 24 hrs by Griess reagent-based assay	[PMID: 30530196]
BV-2	IC₅₀	16.27 μM Compound: NMMA	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 26774654]
BV-2	IC₅₀	16.27 μM Compound: NMMA	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 23149227]
BV-2	IC₅₀	16.32 μM Compound: L-NMMA	Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control	[PMID: 23462643]
BV-2	IC₅₀	16.8 μM Compound: L-NMMA	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess reagent method	[PMID: 19900811]
BV-2	IC₅₀	16.8 μM Compound: L-NMMA	Antiinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 30 mins prior to LPS-challenge measured after 24 hrs by Griess assay Antiinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 30 mins prior to LPS-challenge measured after 24 hrs by Griess assay	[PMID: 23815260]
BV-2	IC₅₀	17 μM Compound: L-NMMA	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay	[PMID: 31082231]
BV-2	IC₅₀	17.6 μM Compound: NG-monomethyl-L-arginine	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 27700070]
BV-2	IC₅₀	18.25 μM Compound: NMMA	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production treated 30 mins before LPS stimulation measured after 24 hrs by Griess reaction	[PMID: 22951040]
BV-2	IC₅₀	18.26 μM Compound: NMMA	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 23707257]
BV-2	IC₅₀	18.3 μM Compound: NMMA	Inhibition of LPS-induced nitric oxide production in mouse BV2 cells treated for 30 mins prior to LPS stimulation for 24 hrs by Griess assay Inhibition of LPS-induced nitric oxide production in mouse BV2 cells treated for 30 mins prior to LPS stimulation for 24 hrs by Griess assay	[PMID: 25466198]
BV-2	IC₅₀	20.53 μM Compound: L-NMMA	Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay	[PMID: 26706168]
BV-2	IC₅₀	20.78 μM Compound: L-NMMA	Anti-neuroinflammatory activity in LPS-activated mouse BV2 cells assessed as inhibition of NO production after 24 hrs by Griess assay Anti-neuroinflammatory activity in LPS-activated mouse BV2 cells assessed as inhibition of NO production after 24 hrs by Griess assay	[PMID: 26988298]
BV-2	IC₅₀	21.25 μM Compound: L-NMMA	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitrite production after 24 hrs by Griess assay	[PMID: 28165740]
BV-2	IC₅₀	21.37 μM Compound: L-NMMA	Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production incubated for 48 hrs by Griess reagent based assay Anti-neuroinflammatory activity in mouse BV-2 cells assessed as inhibition of LPS-induced NO production incubated for 48 hrs by Griess reagent based assay	[PMID: 34546050]
BV-2	IC₅₀	21.4 μM Compound: L-NMMA	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay	[PMID: 30063340]
BV-2	IC₅₀	21.4 μM Compound: L-NMMA	Cytotoxicity against mouse BV2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against mouse BV2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 30232882]
BV-2	IC₅₀	21.8 μM Compound: L-NMMA	Anti-inflammatory in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pre-incubated before LPS challenge for 24 hrs by Griess reagent based assay Anti-inflammatory in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pre-incubated before LPS challenge for 24 hrs by Griess reagent based assay	[PMID: 28358502]
BV-2	IC₅₀	21.9 μM Compound: L-NMMA	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition in LPS-induced NO production preincubated for 30 mins followed b LPS addition and measured after 24 hr by griess reagent based assay Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition in LPS-induced NO production preincubated for 30 mins followed b LPS addition and measured after 24 hr by griess reagent based assay	[PMID: 31625742]
BV-2	IC₅₀	22.1 μM Compound: L-NMMA	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-stimulated nitric oxide production by Griess assay	[PMID: 28262525]
BV-2	IC₅₀	24.3 μM Compound: L-NMMA	Antineuroinflammatory activity against human BV2 cells assessed as inhibition of LPS-induced NO production treated with compound 30 mins and challenged with LPS for 24 hrs Antineuroinflammatory activity against human BV2 cells assessed as inhibition of LPS-induced NO production treated with compound 30 mins and challenged with LPS for 24 hrs	[PMID: 25098650]
BV-2	IC₅₀	24.8 μM Compound: L-NMMA	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay	[PMID: 26812172]
BV-2	IC₅₀	24.8 μM Compound: L-NMMA	Antiinflammatory activity against LPS-stimulated mouse BV2 cells assessed as inhibition of nitric oxide production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess assay Antiinflammatory activity against LPS-stimulated mouse BV2 cells assessed as inhibition of nitric oxide production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess assay	[PMID: 25894905]
BV-2	IC₅₀	26.58 μM Compound: L-NMMA	Anti-inflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay Anti-inflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by griess assay	[PMID: 30472026]
BV-2	IC₅₀	28.8 μM Compound: L-NMMA	Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess assay Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess assay	[PMID: 32520551]
BV-2	IC₅₀	31.8 μM Compound: L-NMMA	Antineuroinflammatory activity against mouse BV-2 cells assessed as inhibition of LPS-induced NO production by Griess assay Antineuroinflammatory activity against mouse BV-2 cells assessed as inhibition of LPS-induced NO production by Griess assay	[PMID: 34964626]
BV-2	IC₅₀	39.28 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in human BV2 cells preincubated for 2 hrs followed by LPS-stimulation measured after 18 hrs by Griess assay Inhibition of LPS-induced NO production in human BV2 cells preincubated for 2 hrs followed by LPS-stimulation measured after 18 hrs by Griess assay	[PMID: 27525351]
DLD-1	IC₅₀	0.3 μM Compound: 8 (L-NMMA)	Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by i-NOS from human DLD-1 cells Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by i-NOS from human DLD-1 cells	[PMID: 11327581]
DLD-1	IC₅₀	0.3 μM Compound: 1	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells	[PMID: 11327580]
HUVEC	IC₅₀	0.3 μM Compound: 8 (L-NMMA)	Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by e-NOS from HUVEC cells Ability to inhibit the conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by e-NOS from HUVEC cells	[PMID: 11327581]
HUVEC	IC₅₀	0.3 μM Compound: 1	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells	[PMID: 11327580]
J774.1	IC₅₀	13.8 μg/mL Compound: L-NMMA	Inhibition of LPS-induced nitric oxide production in mouse J774.1 cells after 24 hrs by griess assay Inhibition of LPS-induced nitric oxide production in mouse J774.1 cells after 24 hrs by griess assay	[PMID: 21428417]
J774.1	IC₅₀	26 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs	[PMID: 12608860]
J774.1	IC₅₀	27.1 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay	[PMID: 15974608]
J774.1	IC₅₀	27.1 μM Compound: L-NMMA	Cytotoxicity against LPS induced mouse J774.1 cells by MTT assay Cytotoxicity against LPS induced mouse J774.1 cells by MTT assay	[PMID: 18154272]
J774.1	IC₅₀	35.7 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in mouse J774.1cells after 24 hrs Inhibition of LPS-induced NO production in mouse J774.1cells after 24 hrs	[PMID: 15104497]
Macrophage	IC₅₀	28 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method Inhibition of LPS-induced NO production in ddY mouse macrophages assessed as nitrate accumulation after 20 hrs by Griess reagent method	[PMID: 12398545]
Macrophage	IC₅₀	57 μM Compound: L-NMMA	Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay	[PMID: 15270564]
RAW	IC₅₀	19.7 μM Compound: L-NMMA	Inhibition of LPS-induced iNOS activity in mouse RAW 264.7 cells assessed as inhibition of NO production pretreated with compound for 30 mins before LPS challenge after 24 hrs by Griess reagent method Inhibition of LPS-induced iNOS activity in mouse RAW 264.7 cells assessed as inhibition of NO production pretreated with compound for 30 mins before LPS challenge after 24 hrs by Griess reagent method	[PMID: 23142320]
RAW	IC₅₀	22.1 μM Compound: L-NG-monomethyl arginine	Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW 264.7 cells pretreated 15 mins before LPS challenge measured after 20 hrs relative to control Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW 264.7 cells pretreated 15 mins before LPS challenge measured after 20 hrs relative to control	[PMID: 21978950]
RAW	IC₅₀	25 μM Compound: 2	Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells Inhibition of radiolabeled arginine conversion to citrulline by isomeric form of Inducible nitric oxide synthase from mouse RAW 264.7 cells	[PMID: 9651169]
RAW264.7	IC₅₀	> 40.5 μM Compound: N-monomethyl-L-arginine	Inhibition of LPS-induced NO release in mouse RAW264.7 cells after 24 hrs by Griess reaction Inhibition of LPS-induced NO release in mouse RAW264.7 cells after 24 hrs by Griess reaction	[PMID: 26052978]
RAW264.7	IC₅₀	> 50 μM Compound: L-NMMA	Cytotoxicity against mouse RAW264.7 cells by MTT assay Cytotoxicity against mouse RAW264.7 cells by MTT assay	[PMID: 16643064]
RAW264.7	IC₅₀	16.11 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as reduction in LPS-induced NO production after 18 hrs by Griess assay	[PMID: 29685654]
RAW264.7	IC₅₀	19.2 μM Compound: L-NMMA	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation after 20 hrs	[PMID: 16643064]
RAW264.7	IC₅₀	19.7 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins measured 24 hrs post LPS challenge by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins measured 24 hrs post LPS challenge by Griess method	[PMID: 22386564]
RAW264.7	IC₅₀	19.7 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins before LPS challenge measured after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins before LPS challenge measured after 24 hrs by Griess method	[PMID: 22115839]
RAW264.7	IC₅₀	19.9 μM Compound: 1, L-NMMA	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 20 hrs by Griess reaction Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 20 hrs by Griess reaction	[PMID: 21377876]
RAW264.7	IC₅₀	2.3 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS activity incubated for 30 mins prior to LPS challenge measured after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS activity incubated for 30 mins prior to LPS challenge measured after 24 hrs by Griess assay	[PMID: 25975638]
RAW264.7	IC₅₀	2.69 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay	[PMID: 26898337]
RAW264.7	IC₅₀	2.73 μM Compound: L-NMMA	Cytotoxicity against mouse RAW264.7 cells assessed as decrease in cell proliferation rate after 24 hrs by MTS assay Cytotoxicity against mouse RAW264.7 cells assessed as decrease in cell proliferation rate after 24 hrs by MTS assay	[PMID: 27777005]
RAW264.7	IC₅₀	21.72 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production incubated for 15 mins prior to LPS challenge measured after 20 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production incubated for 15 mins prior to LPS challenge measured after 20 hrs by Griess assay	[PMID: 24016057]
RAW264.7	IC₅₀	23.5 μM Compound: L-NMMA	Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay Inhibition of lipopolysaccharide-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 30 mins before LPS stimulation for 20 hrs by Griess reagent based assay	[PMID: 27210437]
RAW264.7	IC₅₀	25.1 μM Compound: L-NMMA	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS challenge measured after 20 hrs by Griess assay Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells preincubated for 15 mins followed by LPS challenge measured after 20 hrs by Griess assay	[PMID: 26343828]
RAW264.7	IC₅₀	25.8 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 18 hrs by Griess assay	[PMID: 29400471]
RAW264.7	IC₅₀	27.2 μM Compound: L-NMMA	Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs	[PMID: 31710213]
RAW264.7	IC₅₀	30.92 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production pretreated for 2 hrs followed by LPS-stimulation measured after 20 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production pretreated for 2 hrs followed by LPS-stimulation measured after 20 hrs by Griess assay	[PMID: 28753309]
RAW264.7	IC₅₀	32.9 μM Compound: L-NMMA	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess assay Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 18 hrs by Griess assay	[PMID: 30296083]
RAW264.7	IC₅₀	35.5 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay	[PMID: 33939429]
RAW264.7	IC₅₀	39.2 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 2 hrs prior to LPS-challenge measured after 24 hrs by Greiss assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 2 hrs prior to LPS-challenge measured after 24 hrs by Greiss assay	[PMID: 25924023]
RAW264.7	IC₅₀	40.4 μM Compound: N-monomethyl-L-arginine	Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells by Griess reagent assay Inhibition of nitric oxide production in LPS-stimulated mouse RAW264.7 cells by Griess reagent assay	[PMID: 23394284]
RAW264.7	IC₅₀	44.36 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production incubated for 1 hrs followed by LPS stimulation for 18 hrs by Griess reagent based assay Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production incubated for 1 hrs followed by LPS stimulation for 18 hrs by Griess reagent based assay	[PMID: 35427124]
RAW264.7	IC₅₀	45 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 18 hrs by Griess assay	[PMID: 26741483]
RAW264.7	IC₅₀	73.3 μM Compound: L-NMMA	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production compound pretreated for 1 hr followed by addition of 100 ng/ml LPS for 24 hrs by griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production compound pretreated for 1 hr followed by addition of 100 ng/ml LPS for 24 hrs by griess method	[PMID: 26681510]
Sf9	IC₅₀	0.65 μM Compound: L-NMMA	Inhibition of human eNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method Inhibition of human eNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	[PMID: 22335555]
Sf9	IC₅₀	0.95 μM Compound: L-NMMA	Inhibition of human nNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method Inhibition of human nNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	[PMID: 22335555]
Sf9	IC₅₀	1.8 μM Compound: L-NMMA	Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method Inhibition of human recombinant iNOS expressed in Sf9 cells assessed as reduction in conversion of [3H]L-arginine to [3H]L-citrulline preincubated for 15 mins by radiometric method	[PMID: 22335555]

Molecular Weight

188.23

Formula

C₇H₁₆N₄O₂

CAS No.

17035-90-4

SMILES

OC([C@@H](N)CCCNC(NC)=N)=O

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Please store the product under the recommended conditions in the Certificate of Analysis.

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References

[1]. Frey C, et al., L-thiocitrulline. A stereospecific, heme-binding inhibitor of nitric-oxide synthases. J Biol Chem. 1994 Oct 21;269(42):26083-91. [Content Brief]

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Keywords:

L-NMMA17035-90-4Tilarginine MethylarginineNO SynthaseNitric oxide synthasesNOScompetitiveInhibitorinhibitorinhibit

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L-NMMA (Synonyms: Tilarginine; Methylarginine)

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8 Publications Citing Use of MCE L-NMMA

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Request for HNMR Report