2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. L82-G17

L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity.

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L82-G17 Chemical Structure

L82-G17 Chemical Structure

CAS No. : 92285-87-5

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Based on 1 publication(s) in Google Scholar

L82-G17 Related Antibodies

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Description

L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor. L82-G17 inhibits the third step of the ligation reaction, phosphodiester bond formation. L82-G17can be used as a probe of the catalytic activity[1].

In Vitro

L82-G17 (200 μM, 30 min) has selective uncompetitive inhibitory effect for LigI[1].
L82-G17 (0-100 μM) increases LigI binding to non-ligatable nicked DNA binding[1].
L82-G17 inhibits step 3 of the ligation reaction, phosphodiester bond formation[1].
L82-G17 (0-100 μM) inhibits DNA synthesis, cell viability and s induces DNA damage[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

L82-G17 Related Antibodies

Cell Viability Assay[1]

Cell Line: HeLa cells
Concentration: 0-30 μM
Incubation Time: 5 days
Result: Reduced cell number by about 70% at 20 μM.

Cell Proliferation Assay[1]

Cell Line: CH12F3 WT and CH12F3Δ/Δ cells
Concentration: 0-100 μM
Incubation Time: 72 h
Result: Had great effect on the proliferation and survival of the parental CH12F3 cells.
Molecular Weight

264.67

Formula

C11H9ClN4O2
CAS No.
Appearance

Solid

Color

Pink to orange

SMILES

O=C1NN=CC(N/N=C/C2=CC(O)=CC=C2)=C1Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (472.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.7783 mL 18.8914 mL 37.7829 mL
5 mM 0.7557 mL 3.7783 mL 7.5566 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.86 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7783 mL 18.8914 mL 37.7829 mL 94.4572 mL
5 mM 0.7557 mL 3.7783 mL 7.5566 mL 18.8914 mL
10 mM 0.3778 mL 1.8891 mL 3.7783 mL 9.4457 mL
15 mM 0.2519 mL 1.2594 mL 2.5189 mL 6.2971 mL
20 mM 0.1889 mL 0.9446 mL 1.8891 mL 4.7229 mL
25 mM 0.1511 mL 0.7557 mL 1.5113 mL 3.7783 mL
30 mM 0.1259 mL 0.6297 mL 1.2594 mL 3.1486 mL
40 mM 0.0945 mL 0.4723 mL 0.9446 mL 2.3614 mL
50 mM 0.0756 mL 0.3778 mL 0.7557 mL 1.8891 mL
60 mM 0.0630 mL 0.3149 mL 0.6297 mL 1.5743 mL
80 mM 0.0472 mL 0.2361 mL 0.4723 mL 1.1807 mL
100 mM 0.0378 mL 0.1889 mL 0.3778 mL 0.9446 mL
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L82-G17 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L82-G1792285-87-5DNA/RNA SynthesisDNA ligase I (Ligl)phosphodiester bondprobecatalyticcancerInhibitorinhibitorinhibit

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